Anti-Obesity Effect and Action Mechanism of<i>Adenophora triphylla</i>Root Ethanol Extract in C57BL/6 Obese Mice Fed a High-Fat Diet

Lee, Seong Eun; Lee, Eun-Hei; Lee, Tae-Jong; Kim, Seungwon; Kim, Bae-Hwan

doi:10.1271/bbb.120667

Cited by 26 publications

(13 citation statements)

References 39 publications

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“…Anti-oxidation is nowadays considered as an empirical beneficial function of medicinal plants and foods for chronic disease prevention and health promotion. Relatively, benefits of antioxidants for obesity therapy involving inhibition of adipogenesis-inducing factors like lipid peroxidation and inflammatory cascades are suggested [16,17,18]. Coincidently, associations between anti-oxidation in a lipophilic study model, reduction of TBARS production, and anti-adipogenic effects in animal study models of Nelumbo nucifera [19] and Plantago albicans extract [20] have been reported.…”

Section: Discussionmentioning

confidence: 99%

Phenolic Profile, Antioxidant Activity, and Anti-obesogenic Bioactivity of Mao Luang Fruits (Antidesma bunius L.)

Krongyut

Sutthanut

2019

Molecules

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To investigate the anti-obesity potential of Antidesma bunius L. (MM), a Thai local fruit which is named “Mao Luang,” we have focused on the effects on pancreatic α-amylase and lipase enzyme activity and on adipocyte life cycle using the 3T3-L1 cell line as a model. In addition, the phytochemical composition and anti-oxidation potential were also analyzed using HPLC-PDA UV and colorimetric methods. The ethanolic extract of MM fruits prepared by a maceration method was used in the experiments. MM extract, yield 12.08% w/w, is composed primarily of phenolics and anthocyanins as the major phytochemicals, among which, gallic acid, catechin, anthocyanin-3-glucoside, and protocatechuic acid were initially identified. In addition, susceptibly inhibitory effects on oxidation in a DPPH assay; on lipase enzyme activity rather than amylase enzyme; and on adipocyte adipogenesis of MM were demonstrated. Interestingly, a concentration-dependent bi-modular manner of activity on adipocyte adipogenesis was discovered, whereby a significant anti-adipogenic effect was demonstrated at high concentration, whilst low concentrations of MM showed adipogenic induction. Lipolytic induction was manifested. Conclusively, the ethanolic MM extract was discovered to be a potential anti-obesity agent contributed by inhibitory effects on lipase enzyme and anti-differentiation and -adipogenesis in adipocytes which significantly correlated to the total phenolics content, as well as anti-oxidation as the mechanism of action. Nevertheless, to achieve effective application, further investigation in in vivo models should be considered.

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Section: Discussionmentioning

confidence: 99%

Phenolic Profile, Antioxidant Activity, and Anti-obesogenic Bioactivity of Mao Luang Fruits (Antidesma bunius L.)

Krongyut

Sutthanut

2019

Molecules

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“…Besides its ornamental value, A. triphylla has been an important medicinal plant in oriental medicine for remedying whooping cough and chronic bronchitis in China [1]. In addition, this herb has also been used as a food source to prevent obesity in traditional Korean recipes [3]. In recent years, antitumor and antidiabetic activities have been reported in this species [4, 5].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, antitumor and antidiabetic activities have been reported in this species [4, 5]. Some previous studies attached the importance to extraction and identification of phytochemicals, such as lupenone, daucosterol, and adenophoric acid methyl ester, and to the action of disease resistance [3–5]. However, few studies have focused on the effect of light on the growth and development of this plant species.…”

Section: Introductionmentioning

confidence: 99%

Supplementary Light Source Affects Growth, Metabolism, and Physiology ofAdenophora triphylla(Thunb.) A.DC. Seedlings

Liu

Ren

Jeong

2019

BioMed Research International

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Adenophora triphylla(Thunb.) A.DC., a well-known herbaceous medicinal species, has been reported to protect against human obesity, cancer, and inflammation. Supplementary lighting is a practical strategy to improve crop quality, especially at a propagation stage. However, there has been no study available on the optimal supplementary light source for the commercial production ofA. triphyllaseedlings. In this study, plug seedlings were cultivated in a greenhouse for four weeks under an average daily light intensity of 490μmol·m−2·s−1PPFD coming from the sun and a supplemental lighting (16 h per day) at 120μmol·m−2·s−1PPFD provided by high pressure sodium (HPS), metal halide (MH), far-red (FR) light, white LED (red: green: blue = 2:4:3, LED-w), or mixed (red: green: blue = 4:1:4) LED (LED-mix). The results showed that LED-mix, with a higher percentage of red and blue light, substantially promoted seedling growth compared to other treatments by increasing stem diameter, biomass, specific leaf weight, and root to shoot ratio. The LED-mix also promoted accumulation of soluble sugar, starch, and chlorophyll in the tissue and increased contents of total phenols and flavonoids. Moreover, stomata density and pore area per leaf area under the LED-mix were remarkably greater than those under other treatments. Furthermore, the Western blot analysis revealed that the expression of photosynthetic protein, D1, was notably enhanced by the LED-mix as compared with other light sources. In addition, the LED-mix alleviated the oxidative damage of seedlings by improving enzymatic and nonenzymatic antioxidant systems. Collectively, these results suggest that the LED-mix was the optimal supplementary light source for the production of highest qualityA. triphyllaseedlings.

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“…The Chinese crude drug "shajin", extracted from the roots of A. triphylla, is an effective expectorant for the treatment of whooping cough and chronic bronchitis, and its pharmaceutical name is Radix Adenophorae [1,2]. Historically, A. triphylla was used as food to prevent obesity in Korea [2,3]. In recent years, many more medicinal effects of A. triphylla have been reported, such as anti-cancerous, anti-diabetic, and antioxidant [4].…”

Section: Introductionmentioning

confidence: 99%

“…Although the contents slightly fluctuate depending on the growth condition and genotype of individual variety, it has been proved that A. triphylla holds various phytochemicals, such as β-sitosterol, lupenone, daucosterol, triphyllol, and adenophoric acid methyl ester [5][6][7]. Previous research focused on separation, extract, and characterization of those phytochemicals and on the disease-fighting medicinal mechanisms of A. triphylla [3,[8][9][10]. However, there are no studies available reporting on the growth and physiology of plug seedlings of this species.…”

Section: Introductionmentioning

confidence: 99%

Growth and Physiological Responses of Adenophora triphylla (Thunb.) A.DC. Plug Seedlings to Day and Night Temperature Regimes

Ren

Jeong

et al. 2018

Agronomy

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Adenophora triphylla (Thunb.) A.DC., three-leaf lady bell, is an important medicinal plant used against cancers and obesity. It has been well-established that the temperature regime affects plant growth and development in many ways. However, there is no study available correlating the growth of A. triphylla seedlings with different day and night temperature regimes. In order to find an optimal temperature regime, growth and physiology were investigated in A. triphylla plug seedlings grown in environment-controlled chambers at different day and night temperatures: 20/20 °C (day/night) (TA), 25/15 °C (TB), and 20/15 °C (TC). The seedlings in plug trays were grown under a light intensity of 150 μmol·m−2·s−1 PPFD (photosynthetic photon flux density) provided by white LEDs, a 70% relative humidity, and a 16 h (day)/8 h (night) photoperiod for six weeks. The results showed that the stem diameter, number of roots, and biomass were significantly larger for seedlings in TB than those in TA or TC. Moreover, the contents of total flavonoid, total phenol, and soluble sugar in seedlings grown in TB were markedly higher than those in seedlings in the other two treatments. Soluble protein content was the lowest in seedlings in TC, while starch content was the lowest in seedlings grown in TA. Furthermore, seedlings grown in TB showed significantly lower activities of antioxidant enzymes such as superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase. Native PAGE (polyacrylamide gel electrophoresis) analysis further proved low activities of antioxidant isozymes in TB treatment. Meanwhile, the lowest content of hydrogen peroxide was observed in seedlings grown in TB. In conclusion, the results suggested that the 25/15 °C (day/night) temperature regime is the most suitable for the growth and physiological development of A. triphylla seedlings.

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Anti-Obesity Effect and Action Mechanism ofAdenophora triphyllaRoot Ethanol Extract in C57BL/6 Obese Mice Fed a High-Fat Diet

Cited by 26 publications

References 39 publications

Phenolic Profile, Antioxidant Activity, and Anti-obesogenic Bioactivity of Mao Luang Fruits (Antidesma bunius L.)

Phenolic Profile, Antioxidant Activity, and Anti-obesogenic Bioactivity of Mao Luang Fruits (Antidesma bunius L.)

Supplementary Light Source Affects Growth, Metabolism, and Physiology ofAdenophora triphylla(Thunb.) A.DC. Seedlings

Growth and Physiological Responses of Adenophora triphylla (Thunb.) A.DC. Plug Seedlings to Day and Night Temperature Regimes

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