Anti-Obesity Effect of Rice Bran Extract on High-Fat Diet-Induced Obese Mice

Abstract

Obesity involving adipose tissue growth and development are associated with angiogenesis and extracellular matrix remodeling. Rice bran has antioxidant and cardioprotective properties, and can act as a food supplement with potential health benefits, such as lowering blood pressure, hepatic steatosis, and inflammation. Therefore, we hypothesized that rice bran extract (RBE) can regulate adipose tissue growth and obesity. Male Institute of Cancer Research mice were fed with a high-fat diet (HFD) for 8 weeks and then supplemented with 220 and 1,100 mg/kg/d RBE while the low-fat diet group (control) were not. In addition to body weight, adipose tissue mass, and vessel density, we evaluated the mRNA expression of angiogenic factors such as matrix metalloproteinases, Mmp-2, Mmp-9, and the vascular endothelial growth factor (Vegf) in visceral and subcutaneous adipose tissues using real-time polymerase chain reaction. Administration of RBE to HFD-induced obese mice reduced the body weight and adipose tissue mass compared with untreated mice. It also decreased blood vessel density in the adipose tissue. Furthermore, RBE downregulated Vegf and Mmp-2 mRNA levels in visceral fat tissue. These results demonstrate that RBE, at high concentrations, significantly reduces adipose tissue mass and prevents obesity development in HFD-induced obese mice, which might be partly mediated via an anti-angiogenic mechanism.

Rice Bran Extract Suppresses High-Fat Diet-Induced Hyperlipidemia and Hepatosteatosis through Targeting AMPK and STAT3 Signaling

“…While the metabolic improvement effects (e.g., anti-obesity, anti-hyperlipidemic or anti-hepatosteatosis) of RBEs are well documented [ 8 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ], the optimal extraction conditions enhancing these biological activities have yet to be fully elucidated. As extraction conditions significantly affect bioactivity [ 20 , 39 , 40 ], identifying the optimal conditions and validating their functionality can enhance efficacy, reduce costs, and promote practical applications.…”

“…Its beneficial impact on metabolic health, in particular, has been substantiated in multiple clinical studies. As rice bran is rich in bioactive polyphenols—notably γ-oryzanol, tocopherols, and tocotrienols—it is increasingly acknowledged as a valuable resource for developing nutraceutical agents with a variety of health benefits [ 4 , 6 , 7 , 8 ]. Given these advantages, enhancing the beneficial effects of rice bran through optimized extraction and processing techniques, aimed at maximizing its bioactive components, could augment its physiological impact.…”

Effects of Riceberry Rice Bran Oil Supplementation on Oxidative Stress and Cardiovascular Risk Biomarkers in Older Adults with Prehypertension

“…HRV and BP variables did not change after either form of supplementation, and no differences were found between the groups. Supplementation with rice bran and its active components, such as oryzanols, tocopherols, tocotrienols, phytosterols, and nucleotides, has been shown in animal experiments to be effective against chronic diseases such as hypertension, dyslipidemia, diabetes mellitus, metabolic syndrome, obesity, and inflammation (Ardiansyah et al, 2009;Palou et al, 2015;Wang et al, 2015;Alauddin et al, 2016;Duansak et al, 2022;Munkong et al, 2022).…”

Composition, Microbiota, Mechanisms, and Anti-Obesity Properties of Rice Bran

“…Moreover, RBWE supplementation significantly reduced the adipose tissue mass and vascular endothelial growth factor (VEGF) and MMP-2 expressions in visceral fat tissue [ 95 ]. The study claimed that RBWE could reduce obesity-associated hypertension and adipose tissue mass and improve health status [ 103 , 104 ].…”

“…RB intervention significantly improved adipogenesis, reduced adipocyte size and adipose tissue mass, reduced adipocyte hypertrophy and lipid accumulation, and expressions of VEGF and MMP-2 in visceral fat tissue [ 89 , 93 , 97 , 103 , 104 ].…”