Anti-osteosarcoma effect of antiserum against cross antigen TPD52 between osteosarcoma and Trichinella spiralis

Abstract

Background Trichinella spiralis (T. spiralis) is a parasite occurring worldwide that has been proven to have antitumour ability. However, studies on the antitumour effects of cross antigens between the tumour and T. spiralis or antibodies against cross antigens between tumours and T. spiralis are rare. Methods To study the role of cross antigens between osteosarcoma and T. spiralis, we first screened the cDNA expression library of T. spiralis muscle larvae to obtain the cross antigen gene tumour protein D52 (TPD52), and prepared fusion protein TPD52 and its antiserum. The anti-osteosarcoma effect of the anti-TPD52 antiserum was studied using cell proliferation and cytotoxicity assays as well as in vivo animal models; preliminary data on the mechanism were obtained using western blot and immunohistochemistry analyses. Results Our results indicated that TPD52 was mainly localized in the cytoplasm of MG-63 cells. Anti-TPD52 antiserum inhibited the proliferation of MG-63 cells and the growth of osteosarcoma in a dose-dependent manner. The tumour inhibition rate in the 100 μg treatment group was 61.95%. Enzyme-linked immunosorbent assay showed that injection of anti-TPD52 antiserum increased the serum levels of IFN-γ, TNF-α, and IL-12 in nude mice. Haematoxylin and eosin staining showed that anti-TPD52 antiserum did not cause significant pathological damage. Apoptosis of osteosarcoma cells was induced by anti-TPD52 antiserum in vivo and in vitro. Conclusions Anti-TPD52 antiserum exerts an anti-osteosarcoma effect by inducing apoptosis without causing histopathological damage. Graphical abstract

Identifying squalene epoxidase as a metabolic vulnerability in high‐risk osteosarcoma using an artificial intelligence‐derived prognostic index

“…FDPS promotes prostate cancer progression, 69 hinting at its potential unfavourable role in OSA. The observed anti‐OSA effects of anti‐TPD52 antiserum in vivo warrant attention 70 . Conversely, PMEPA1 , which suppresses the TGF‐beta signalling pathway, 71 might act as an OSA suppressor, especially given this pathway's oncogenic roles in OSA 72 .…”

“…The observed anti‐OSA effects of anti‐TPD52 antiserum in vivo warrant attention. 70 Conversely, PMEPA1 , which suppresses the TGF‐beta signalling pathway, 71 might act as an OSA suppressor, especially given this pathway's oncogenic roles in OSA. 72 The promoting role of the WNT signalling pathway in OSA 73 also makes CTNNBIP1 , a WNT pathway antagonist, 74 a potential OSA suppressor.…”

Prophylactic antineoplastic activity of Toxoplasma gondii RH derived antigen against ehrlich solid carcinoma with evidence of shared antigens by comparative immunoblotting

“…While the immunomodulatory and antiangiogenic potency of ATV has been investigated, the additional theory of shared antigens between ATV and cancer cells was next to be considered. Shared antigens and their cross reactivity were confirmed between different parasites and cancer cells [ 5 , 49 – 51 ]. Mucin-type O-glycan structures (Tn, sial Tn, TF, Tk) are among the most specific cancer associated antigens, which are vital in malignant cell invasion and metastasis.…”

Trichinella spiralis Larval Extract as a Biological Anti‐Tumor Therapy in a Murine Model of Ehrlich Solid Carcinoma

“…Furthermore, previous studies have investigated the potential of T. spiralis and their products to prolong the survival time of tumor-bearing host, reduce tumor growth in vivo and induce tumor apoptosis in vitro [4,16,25,[39][40][41][42][43][44][45][46][47].…”