Anti-oxidant activity of seedlings from rice seeds stored in different temperatures over 10 years

Abstract

Seed deterioration can occur in premature maturation and bad storage conditions. In these two cases, the excessive production of the reactive oxygen species (ROS) has contributed for losses in physiological potential seeds. Thus, the objective of this study was to evaluate the physiological potential and antioxidant enzymes in rice seedlings against 10 years storage conditions at different temperatures. The cultivars BRS Pelota, BRS Atalanta and BRS Firmeza were kept in waterproof bag at-15, 1 and 18 ºC until evaluation. Then, the physiological potential and antioxidant enzymes (SOD, CAT and APX) were estimated. Storage at-15 and 1 ºC provided the best physiological potential conditions of cv. BRS Pelota, Atalanta and Firmeza. These results were compatible with the enzymatic activity of seedlings from seeds stored at different temperatures. Storage at 18 °C was detrimental to the maintenance of physiological potential of the cv. BRS Pelota, Atalanta and Firmesa over 10 years. The lower activity of antioxidant defense system enzymes the higher seed liveliness.

Physiological potential and antioxidant metabolism during storage of soybean seeds contrasting with phenylpropanoid pathway compounds

“…It was used 0.05 mL of seed coat extract and 0.025 mL of the embryo. For quantification, 5 mL of Bradford reagent was added, and the samples were left to rest for 15 minutes; then, readings were taken in the spectrophotometer at 595 nm; -SOD activity: determined by the addition of several solutions in the following order: 1 mL of 50 mM potassium phosphate -pH 7.8, 0.4 mL of 14 mM methionine, 0.020 mL of 0.1 µM EDTA, 0.31 mL of Mili-Q water, 0.15 mL of nitrotetrazolium blue (NBT) 75 µM, 0.1 mL of enzymatic extract (sample) and finally 0.06 mL of 2 µM riboflavin, according to Krüger et al (2017), with amendments. The reaction was carried out under the light of a 15-W fluorescent lamp kept inside a box.…”