Anti-oxidant and immunomodulatory properties of seabuckthorn (Hippophae rhamnoides)—an in vitro study

Subramanian, Geetha; Ram, M. Sai; Singh, Virendra; Ilavazhagan, G.; Sawhney, R. C.

doi:10.1016/s0378-8741(01)00406-8

Cited by 212 publications

(131 citation statements)

References 11 publications

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“…Spectral data of compounds (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) 1 H-NMR spectral data were similar to those reported in literature (19) . C NMR spectral data were similar to those reported in literature (12) .…”

Section: Isolation and Purification Of Phenolics From L Nobilis L Woodsupporting

confidence: 81%

In vitro Anti-inflammatory, Cytotoxic and Hepatoprotective Activities of Laurus nobilis L. (Lauraceae) Wood Extract and Its Constitutive Phenolics

2013

Egypt. J. Chem.

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HE POSSIBLE anti-inflammatory, cytotoxic and hepatoprotective activities of Laurus nobilis L. wood extract was in vitro evaluated. The anti-inflammatory activity was assessed by measuring Nitric Oxide (NO) production by the inflammagen lipopolysacchride. The extract was evaluated for its cytotoxic activity on six different human cancer cell lines together with the normal non-malignant melanocytes cell line (HFB4) using the SRB assay. The hepatoprotective activity against paracetamol toxicity was determined using primary cultured rat hepatocytes. The extract showed moderate anti-inflammatory activity as shown in the amount of nitric oxide produced with a level of 4.3 μM /ml (67 % inhibition), in comparison to the potent anti-inflammatory drug Dexamethasone (95 % inhibition). The extract was found to have moderate cytotoxic activity against the tumor cell lines used at the applied concentrations with IC 50 ranging from 15.5 -47.6 µg/ml compared to the potent cytotoxic drug doxorubicin. The wood extract showed hepatoprotective activity against paracetamol toxic effect at concentration of 20g/ ml. The constitutive phenolics of L. nobilis L. wood were studied and led to the separation and identification of 12 compounds, all isolated for the first time from L. nobilis L. wood and were identified using chemical, conventional and advanced spectral techniques.

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Section: Isolation and Purification Of Phenolics From L Nobilis L Woodsupporting

confidence: 81%

In vitro Anti-inflammatory, Cytotoxic and Hepatoprotective Activities of Laurus nobilis L. (Lauraceae) Wood Extract and Its Constitutive Phenolics

2013

Egypt. J. Chem.

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“…잘 익은 열매는 비타민 A, C, E와 K, carotenoids, 그리고 organic acids가 풍부하며 (Chen et al 1990;Yao and Tigerstedt 1992;Pintea et al 2001;Kallio et al 2002a), 강력한 항산화 (Eccleston et al 2002;Suleyman et al 2002), 궤양 유발 억제 (Suleyman et al 2001) 및 광 보호 효과 (Goel et al 2002)를 보인다. 펄프 오일과 종자 오일은 면역조절, 항산화 및 항미 생물 활성을 가지며 (Geetha et al 2002;Negi et al 2005), 위 궤양 (Suleyman et al 2001;Xing et al 2002), 피부 장애 (Yang et al 2000), 관상 동맥 질환 (Eccleston et al 2002), 방사선 노 출에 의한 산화적 피해 (Goel et al 2005 Isorhamnetin, kaempferol과 quereetin을 포함한 비타민나무 의 flavonoid는 다양한 약리효과 (항산화 , 세포독성 (Khatab et al 2006), 항암 (Fu et al 2004;Chen and Kang 2005;Niering et al 2005) 및 reverse multi-drug resistance activity (Chung et al 2005 (Fig. 4) …”

Section: 비타민나무의 약리 효과unclassified

Phamalogical effect and component of sea buckthorn(Hippophae rhamnoides L.)

김주성¹,

유창연²,

김명조³

2010

Journal of Plant Biotechnology

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Sea buckthorn (Hippophae rhamnoides L.) is deciduous shrubs in the genus Hippophae, mainly cultivated in Europe and Asia. Sea buckthorn berries have a high vitamin C, vitamin E, carotenoids, carbohydrates, protein, organic acids, dietary minerals, triterpenoids, polyphenolic acids and amino acids. Extracts of sea buckthorn berries have anti-obesity, anti-oxidantive, anti-microbial, anti-ulcerogenic, anti-diabetic and nutritional effects. Sea buckthorn used as a traditional medicine for the treatment of cough, aid digestion, invigorate blood circulation and alleviate pain. Extracts of sea buckthorn branches and leaves was administered to humans and animals to treat gastrointestinal distress in Mongolia. This paper briefly reviews the most relevant experimental data on the pharmacological effects and isolated component of sea buckthorn. And, we also describe the importance of sea buckthorn as the environmental-friendly crops.

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“…Seabuckthorn oils are rich in fatty acids and vitamins. The various in vivo study of seabuckthorn oil reported to have antitumour 7,8 ,antioxidant 9, 10 , anti-microbial 11,12 , anti-ulcer properties 8 and hepato-protective 8,13 and for treating cardiovascular diseases 8 .The oil has several other pharmacological actions such as diminishing inflammations, relieving pain, reducing the toxic effects of traditional drugs, skin tissue regeneration after mechanical, chemical and burn injuries, used in skin grafting treatment of corneal wounds;antimutagen effects 12 . Besides its medicinal use, seabuckthorn berries can be processed into juices as a nutritional supplement.…”

Section: Introductionmentioning

confidence: 99%

A Convenient and Industrially Viable Route to Separate Lipophilic and Hydrophilic Fractions of Seabuckthorn Pulp and Analysis of Their Activities

Pal¹,

Malhotra²,

Naik³

2016

Int. Res. J. Pharm.

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Traditionally, the extraction of oil from the Seabuckthorn pulp was done by first separating out the juice by mechanically pressing the berries followed by centrifugal separation of oil from the juice by density difference. But the pressed pulp cake still contained both the oil and the juice to some extent. Thus, a complete separation of the lipophilic (oil) and hydrophilic (juice) fractions of the pulp was not achieved. The process that we present in this work separates both the oil and juice fractions very discretely by directly processing the pulp as a whole in an alcoholic medium to separate the lipophilic and the vitamin-rich hydrophilic fractions embedded in the pulp. Moreover, we achieve this separation without employing expensive machines like mechanical presses and high-speed centrifuges. Further, the extraction of the oil from lipophilic part has been done by both the solvent and supercritical extraction methods followed by a comparative study using HPLC analysis of the bio-actives obtained from both the methods. We found that the carotenoids extracted better with the solvent extraction, while sterols extracted better with the supercritical extraction. The overall yield of the oil and the bio-actives was comparable to that of the earlier methods. The aqueous alcohol soluble fraction was evaluated for vitamin C, total acids and reducing sugars. We conclude that our process is convenient, cost-effective and good in terms of yield and recovery of the bio-actives, which makes it industrially applicable. This was endorsed by a successful pilot study trial of a 10 kg batch conducted at SSP

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Anti-oxidant and immunomodulatory properties of seabuckthorn (Hippophae rhamnoides)—an in vitro study

Cited by 212 publications

References 11 publications

In vitro Anti-inflammatory, Cytotoxic and Hepatoprotective Activities of Laurus nobilis L. (Lauraceae) Wood Extract and Its Constitutive Phenolics

In vitro Anti-inflammatory, Cytotoxic and Hepatoprotective Activities of Laurus nobilis L. (Lauraceae) Wood Extract and Its Constitutive Phenolics

Phamalogical effect and component of sea buckthorn(Hippophae rhamnoides L.)

A Convenient and Industrially Viable Route to Separate Lipophilic and Hydrophilic Fractions of Seabuckthorn Pulp and Analysis of Their Activities

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