Anti-Oxidative and Anti-Inflammatory Effects of QGC in Cultured Feline Esophageal Epithelial Cells

Abstract

Quercetin-3-O-β-D-glucuronopyranoside (QGC) is a flavonoid glucoside extracted from Rumex Aquaticus Herba. In the present study, anti-oxidative and anti-inflammatory effects of QGC were tested in vitro. Epithelial cells obtained from cat esophagus were cultured. When the cells were exposed to acid for 2 h, cell viability was decreased to 36%. Pretreatment with 50 µM QGC for 2 h prevented the reduction in cell viability. QGC also inhibited the productions of intracellular ROS by inflammatory inducers such as acid, lipopolysaccharide, indomethacin and ethanol. QGC significantly increased the activities of superoxide dismutase (SOD) and catalase, and also induced the expression of SOD2, while it restored the decrease of catalase expression in cells exposed to acid. QGC inhibited NF-κB translocation, cyclooxygenase-2 expression and PGE2 secretion in cells exposed to acid, which plays an important role in the pathogenesis of esophagitis. The data suggest that QGC may well be one of the promising substances to attenuate oxidative epithelial cell injury and inflammatory signaling in esophagus inflammation.

Antioxidant and Anti‐Inflammatory Effects of Rhei Rhizoma and Coptidis Rhizoma Mixture on Reflux Esophagitis in Rats

“…The action of oxidative stress in inflammation-based GI tract diseases was widely known and, in the development of RE, it also results to be more important than the injuries caused by gastric acids [ 8 , 41 ]. An imbalance in the generation of free radicals like ROS and reactive nitrogen species such as ONOO − (which is highly toxic) was found to be responsible for the esophageal tissue damage; this finding was supported by the studies showing that tissue damage could be prevented with the use of antioxidant agent [ 42 ]. ROS causes oxidative damage in cellular components such as DNA, proteins, and membrane lipids.…”

Proinflammatory Pathways: The Modulation by Flavonoids

“… The EGCG effects in inflammation were reviewed in . An increasing number of in vitro and in vivo studies that describe the anti‐inflammatory effects of flavonoids glycosides has being published, showing the rising interest in these molecules …”

“…113 An increasing number of in vitro and in vivo studies that describe the antiinflammatory effects of flavonoids glycosides has being published, showing the rising interest in these molecules. 105,[114][115][116][117][118][119][120][121][122][123] Another flavonoid derivative, a flavoalkaloid, named lilaline (3,4 ,5,7-tetrahydroxy-8-(4methyl-5-oxo-2-pyrrolidinyl)-flavone), isolated from the flowers of Lilium candidum L., was described as having anti-inflammatory properties through the inhibition of COX enzyme. 124…”

Serum adenosine deaminase, catalase, and carbonic anhydrase activities in patients with renal cell carcinoma

“…Some defense mechanisms in the body prevent the development of free radicals and the damage they cause. One of the most important of these antioxidant enzymes is superoxide dismutase, which catalyzes the dismutation of superoxide anion into hydrogen peroxide (H 2 O 2 ), which is removed by catalase (CAT), a well-known plasma antioxidant enzyme, 4 and glutathione peroxidase.…”