Anti-oxidative and anti-proliferative activities of extracted phytochemical compound thymoquinone

Butt, Ayesha; Nisar, Numrah; Mughal, Tahira Aziz; Ghani, Nadia; Altaf, Imran

doi:10.5455/jpma.302643156

Cited by 13 publications

(8 citation statements)

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“…against two human cancer cell lines: the colonic (HCT116) and breast adenocarcinoma (MCF7), respectively. Butt et al [39] demonstrated that the TEO was found to be a good source of thymoquinone as a chemotherapeutic drug that expressed potent antioxidant and antiproliferative activities. To date, no study indicated the TEO positive effects on memory function in zebrafish against Sco-induced cognitive dysfunction by regulating cholinergic and antioxidant pathways.…”

Section: Introductionmentioning

confidence: 99%

Thymus vulgaris Essential Oil Protects Zebrafish against Cognitive Dysfunction by Regulating Cholinergic and Antioxidants Systems

et al. 2020

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Thymus vulgaris L. is an aromatic herb used for medicinal purposes such as antimicrobial, spasmolytic, antioxidant, anti-inflammatory, antinociceptive, antitumor, and may have beneficial effects in the treatment of Alzheimer’s disease. The present study aimed to investigate whether Thymus vulgaris L. essential oil enhances cognitive function via the action on cholinergic neurons using scopolamine (Sco)-induced zebrafish (Danio rerio) model of memory impairments. Thymus vulgaris L. essential oil (TEO, 25, 150, and 300 µL/L) was administered by immersion to zebrafish once daily for 13 days, whereas memory impairment was induced by Sco (100 μM), a muscarinic receptor antagonist, delivered 30 min before behavioral tests. Spatial memory was assessed using the Y-maze test and novel object recognition test (NOR). Anxiety and depression were measured in the novel tank diving test (NTT). Gas Chromatograph-Mass Spectrometry (GC-MS) analysis was used to study the phytochemical composition of TEO. Acetylcholinesterase (AChE) activity and oxidative stress response in the brain of zebrafish were determined. TEO ameliorated Sco-induced increasing of AChE activity, amnesia, anxiety, and reduced the brain antioxidant capacity. These results suggest that TEO may have preventive and/or therapeutic potentials in the management of memory deficits and brain oxidative stress in zebrafish with amnesia.

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Section: Introductionmentioning

confidence: 99%

Thymus vulgaris Essential Oil Protects Zebrafish against Cognitive Dysfunction by Regulating Cholinergic and Antioxidants Systems

et al. 2020

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“…On the other hand, Butt and co-authors (2019) have evaluated the anti-oxidant and anti-proliferative potential of Thymoquinone extracted from the essential oil of indigenous herbs of Nigella sativa and Thymus vulgaris [3]. Cell proliferation of HeLa cancer significantly decreased with a dose-response manner (p<0.01), showing the highest cell death in a high concentration of Thymoquinone.…”

Section: Fig 2 Effect Of Th Pulegioides Extract (5 Mg/ml) On the Hcl-induced Hemolysis In Vitro Of The Equine Erythrocytes (M ± M N = 18)mentioning

confidence: 99%

“…Inhibitory concentration 50 (IC 50 ) of cancer cell line treated with Nigella sativa oil was 0.5 μM and Thymus vulgaris was 18 μM compared to standard Thymoquinone, showing Inhibitory concentration (IC 50 ) of 6 μM. Researchers concluded that both Nigella sativa and Thymus vulgaris were found to be the best source of Thymoquinone as chemotherapeutic drugs expressed potent anti-oxidant and anti-proliferative activities [3].…”

Section: Fig 2 Effect Of Th Pulegioides Extract (5 Mg/ml) On the Hcl-induced Hemolysis In Vitro Of The Equine Erythrocytes (M ± M N = 18)mentioning

confidence: 99%

Evaluation of Oxidative Stress Biomarkers in Equine Erythrocytes After in Vitro Treatment With Leaf Extract of Thymus Pulegioides L. (Lamiaceae)

Tkachenko

Kurhaluk

Honcharenko

et al. 2021

STBIAS

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In line with our previous study, we continue to evaluate the antioxidant potential of four species and one interspecific hybrid of the Thymus genus sampled in the Western part of Ukraine on the equine erythrocyte model. Therefore, in the present study, the oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), carbonyl derivatives content of protein oxidative modification, total antioxidant capacity (TAC)], as well as HCl-induced hemolysis in the equine erythrocytes, was used for assessing the antioxidant activity of extract obtained from the leaves of Thymus pulegioides L. in dose 5 mg/mL. Leaves of Th. pulegioides were collected among grass nearby land parcels (Syvky village, Bilohirya district, Khmelnytsky region, Ukraine; N 50°02´02,8´´, E 26°14´13,9´´, 306 m a.s.l.). Equine erythrocyte aliquots were used in the study. For positive control (blank), phosphate buffer was used. After incubation of the mixture at 37°C for 60 mins with continuous stirring, samples were used for the biochemical assays. Results of the current study revealed that the extract obtained from leaves of Th. pulegioides (5 mg/mL) has a mild cytotoxic activity on the equine erythrocytes increasing the level of lipid peroxidation biomarker and hemolysis rate. The investigation also revealed that this extract exhibited hemolytic activity. These findings suggest the use of Th. pulegioides extract in dose 5 mg/mL as a source of prooxidant compounds and warrant further studies to evaluate their therapeutic potential. The aldehydic and ketonic derivatives level, as well as total antioxidant capacity, was non-significantly altered after in vitro incubation with an extract obtained from leaves of Th. pulegioides. Screening of Thymus species for other biological activities including antioxidant activities is essential and may be effective for searching the preventive agents in the pathogenesis of some diseases as well as prevention and treatment of some disorders in medicine and veterinary.

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“… 2015 ; Butt et al. 2019 ). Other active constituents have been found in BS are 4-terpineol, α-pinene, carvacrol, limonene, longifolene, p-cymene, t-anethole benzene and thymol (Kooti et al.…”

Section: Introductionmentioning

confidence: 99%

Effects of garden cress, fenugreek and black seed on the pharmacodynamics of metoprolol: an herb-drug interaction study in rats with hypertension

Jardan

Ahad

Raish

et al. 2021

Pharmaceutical Biology

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Context Garden cress (GC), fenugreek (FG), and black seed (BS) are traditional herbal medicine for managing hypertension. Objective The effects of the three herbs on the pharmacodynamics of metoprolol tartrate (MT) in hypertensive rats were investigated. Materials and methods Wistar rats were divided in five groups ( n = 6). Group I served as normal control group and Group II (hypertensive control group) had rats treated orally with N -nitro L -arginine methyl ester ( L -NAME, 40 mg/kg/day) only. Groups III, IV, and V rats were orally treated with L -NAME (40 mg/kg/day) + GC (300 mg/kg, once daily), L -NAME (40 mg/kg/day) + FG (300 mg/kg, once daily) and L -NAME (40 mg/kg/day) + BS (300 mg/kg, once daily), respectively, for 2 weeks, and on the 14th day, blood pressure and heart rate were recorded using a tail-cuff blood pressure-measuring system. On the 16th day, a single dose of MT (10 mg/kg) was orally administered, and the rats’ blood pressure and heart rate were recorded. Results GC, FG, and BS decreased systolic blood pressure (SBP) by 8.7%, 8.5%, and 8.7%, respectively, in hypertensive rats. A greater decrease in SBP by 14.5%, 14.8%, and 16.1% was observed when hypertensive rats were treated with L -NAME + GC + MT, L -NAME + FG + MT, and L -NAME + BS + MT, respectively. Similarly, hypertensive rats treated with the combination of herbs and MT had significantly lower diastolic blood pressure (DBP) than those treated with herbs alone and those treated with L -NAME alone. Conclusions The combination of investigated herbs and MT had a beneficial effect on hypertension. However, the concurrent administration of drugs, particularly those predominantly cleared through CYP450 2D6-catalyzed metabolism, with the three investigated herbs should be considered with caution.

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Anti-oxidative and anti-proliferative activities of extracted phytochemical compound thymoquinone

Cited by 13 publications

References 0 publications

Thymus vulgaris Essential Oil Protects Zebrafish against Cognitive Dysfunction by Regulating Cholinergic and Antioxidants Systems

Thymus vulgaris Essential Oil Protects Zebrafish against Cognitive Dysfunction by Regulating Cholinergic and Antioxidants Systems

Evaluation of Oxidative Stress Biomarkers in Equine Erythrocytes After in Vitro Treatment With Leaf Extract of Thymus Pulegioides L. (Lamiaceae)

Effects of garden cress, fenugreek and black seed on the pharmacodynamics of metoprolol: an herb-drug interaction study in rats with hypertension

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