Anti-oxidative effects of citro flavonoids on spermatogenesis in rat

Abstract

Citrus fruits have long been recognized as containing valuable sources of important nutrients which are biologically active in humans. Citrus fruits, such as oranges, contain compounds called phytochemicals that can be included into three major groups: the flavonoids, limonoids and carotenoids. The flavonoids are a group of benzopyran derivatives which occur widely in plants. The flavonoids typically consist of a benzene ring fused with the heterocyclic six-membered ring containing an oxygen atom. As citrus has an antioxidant's potential, we want to evaluating its useful effect on spermatogenesis and sperm parameters. Wistar male rat (n=30) were allocated into three groups, control (n=10) and test groups (n=20), that subdivided into groups of 2 that received citrus extract powder (400 and 600 mg/rat) for 30 consequence day. Animals were kept in standard conditions. In twentieth day the testes tissue of rats in whole groups were removed and sperm was collected from epididymis and prepared for analysis. TAC, SOD levels and percentage of sperm viability and motility in both test groups significantly increased (p<0.05) in comparison to control group, whereas, sperm concentration, morphology and testes weights in both experimental and control group were similar. The level of MDA in both extract groups were significantly decreased (p<0.05). Results revealed that administration of 600 mg/kg/day citrus extract significantly increased the TAC, SOD levels and sperm percentage, viability, motility and decreased MDA levels. This suggested that citrus may be promising in enhancing sperm healthy parameters.

Protective Effects of Quercetin on Selected Oxidative Biomarkers in Bovine Spermatozoa Subjected to Ferrous Ascorbate

“…Complementary our results, SOD levels, sperm viability and motility increased in experiments designed by Khaki et al. (). In addition, Nass‐Arden and Breitbart () showed that QUE administration to fresh ram semen temporarily inhibited glycolysis, thereby preventing acidification and enhancing longevity of the germ cells.…”

“…Khaki et al. () reported on the beneficial and protective effects of QUE on the sperm viability, motility and total serum testosterone in chemically induced diabetic rats. Studies focused on the effects of QUE on oxidative damage in cultured chicken spermatogonial cells showed that at 1 μg/ml, it increased the cell concentration and reduced the aroclor‐induced testicular oxidative damage (Mi and Zhang ).…”

“…Furthermore, QUE exhibited protective and ROS-scavenging actions in spermatozoa incubated with TBHP, which are in accordance with our luminometric and colorimetric results related to the free radical generation by bovine spermatozoa. Khaki et al (2011) reported on the beneficial and protective effects of QUE on the sperm viability, motility and total serum testosterone in chemically induced diabetic rats. Studies focused on the effects of QUE on oxidative damage in cultured chicken spermatogonial cells showed that at 1 lg/ml, it increased the cell concentration and reduced the aroclor-induced testicular oxidative damage (Mi and Zhang 2005).…”

A biochemical and histological approach to study antifertility effects of methanol leaf extract of Asplenium dalhousiae Hook. in adult male rats

“…Dandekar, Nadkarni, Kulkarni, and Punekar () have reported that due to higher content of polyunsaturated fatty acids in the plasma membrane of spermatozoa, they are highly susceptible to elevated levels of ROS (Dandekar et al, ). With disturbed levels of antioxidants, lipid peroxidation is induced and, thus, disruption of lipid membranes occurs, resulting in reduced sperm motility and decreased spermatogenesis (Khaki, ). Our study has also shown dose‐dependent increase in number of reactive oxygen species and induction of spermatogenic arrest due to actions of quercetin in testicular tissue.…”

Pleurotus tuber-regium mushrooms in the diet of rats ameliorates reproductive and testicular injury caused by carbon tetrachloride

“…In sub Saharan Africa, there is lack of data on the true incidence of MSD but researchers believed that it is higher than 60% [2]. MSD is a syndrome with many diseases that usually but not always involve problems of sperm concentration, morphology and motility, hormonal imbalance that could result from low levels of testosterone, aging process, and drugs like antidepressants or environmental chemicals and cigarettes [3][4][5]. Carbon tetrachloride is a well-known industrial solvent that had been banned for its toxicological concerns and is known to be a toxicant to the liver, kidney, lungs, testis etc.…”