Anti-pathogenic and probiotic attributes of Lactobacillus salivarius and Lactobacillus plantarum strains isolated from feces of Algerian infants and adults

Seddik, Hamza Ait; Bendali, Farida; Cudennec, Benoît; Drider, Djamel

doi:10.1016/j.resmic.2016.12.003

Cited by 38 publications

(21 citation statements)

References 32 publications

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“…Of note, the invasion of eukaryotic Caco-2 cells by L. monocytogenes, which is an intracellular pathogen, can be as well affected by these adherent Lactobacillus strains. The data gathered from the exclusion test are promising since they pointed out a clear protective effect against these pathogens, mainly by impeding their adhesion on the Caco-2 monolayer model, which strengthens our former report [34]. Of note, the abilities to hamper pathogens adhesion to the host tissues is as a key hurdle, and can therefore prevent their invasion process or limit some of their adverse effects, such as the destabilization of intercellular junctions [62].…”

Section: Discussionsupporting

confidence: 77%

“…The volume of injected sample was 25 μL, a calibration curve of pure lactic acid (Sigma-Aldrich) with concentrations of 1, 5, 10, 15 and 20 g/L was carried out to establish the correlation between data obtained from chromatographic peaks area and produced lactic acid concentration. The peak corresponding to lactic acid, which is eluted at 5.32 min [ 34 ], was identified using the Azur software.…”

Section: Methodsmentioning

confidence: 99%

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Clusters of Lactobacillus Strains from Vegetal Origins Are Associated with Beneficial Functions: Experimental Data and Statistical Interpretations

Barache

Belguesmia

Ladjouzi

et al. 2020

Foods

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Nine strains of Lactiplantibacillus plantarum and one strain of Lacticaseibacillus paracasei that were recently isolated from prickly pears, fresh figs and blackberries, which are traditionally and largely consumed fruits in Kabylia (north of Algeria), were studied here for their antagonism and antioxidant properties as well as for production of exopolysaccharides. With respect to their inhibitory properties, these strains were tested against three food representative pathogens including Escherichia coli ATCC 8739, Staphylococcus aureus 2S6 and Listeria monocytogenes 162. The antagonism of these pathogens was attributable to lactic acid production, present in the cell free supernatant, at concentrations ranging from 9 to 16.74 g/L. The anti-adhesive properties observed on polystyrene or eukaryotic Caco-2 cells were exerted in a strain dependent-manner. Indeed, the scores obtained ranged from 27% to 75% for S. aureus 2S6, 54% to 95% for L. monocytogenes 162, and 50% to 97% for E. coli ATCC 8739. The co-aggregation of these Lactobacillus strains with the aforementioned target bacteria appeared to be exerted in a strain-dependent manner, with noticeably the upmost rate for Lb. paracasei FB1 on S. aureus 2S6. Interestingly, these novel Lactobacillus strains were able to produce a large amount (315.55 to 483.22 mg/L) of exopolysaccharides, and showed a significant scavenging activity on the 2,2-di-phényl-2-picrylhydrazyle (DPPH) synthetic free radical with rates of 51% to 56%. Of note, the highest antioxidant activity was observed for Lb. paracasei FB1 using the culture supernatants, intact cells or the intracellular extract. The statistical analysis of these data using the principal component analysis (ACP) enabled us to establish three distinct clusters with potential applications as bioprotective and/or probiotic agents, following further evaluation.

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Section: Discussionsupporting

confidence: 77%

Section: Methodsmentioning

confidence: 99%

Clusters of Lactobacillus Strains from Vegetal Origins Are Associated with Beneficial Functions: Experimental Data and Statistical Interpretations

Barache

Belguesmia

Ladjouzi

et al. 2020

Foods

Self Cite

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“…Lab. plantarum can be isolated from human breast milk (Jiang et al, 2016), human feces (Ait Seddik et al, 2017; Costa et al, 2014), dairy products (Jabbari et al, 2017; Papadopoulou et al, 2018), and Asian fermented fruits and vegetables (Arasu and Al-Dhabi, 2017; Chen et al, 2010; Endo et al, 2008; Leisner et al, 2001; Patra et al, 2016; Swain et al, 2014; Tamang et al, 2005). Interestingly, the function and efficacy of probiotics depend on the strain, and may vary by the isolation sources.…”

Section: Introductionmentioning

confidence: 99%

Probiotic BSH Activity and Anti-Obesity Potential of Lactobacillus plantarum Strain TCI378 Isolated from Korean Kimchi

Huang

Chen

et al. 2019

pnf

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Lactobacillus (Lab.) is a human probiotic beneficial for the prevention and improvement of disease, yet properties of different Lab. strains are diverse. To obtain a Lab. strain that possesses greater potential against gastrointestinal dysfunction, we isolated Lactobacillus plantarum TCI378 (TCI378) from naturally fermented Korean kimchi. TCI378 has shown potential as probiotic since it can survive at pH 3.0 and in the presence of 0.3% bile acid. The bile salt hydrolase activity of TCI378 was shown by formation of opaque granular white colonies on solid de Man Rogosa Sharpe (MRS) medium supplemented with taurodeoxycholic acid, and its cholesterol-lowering ability in MRS medium supplemented with cholesterol. The metabolites of TCI378 from liquid culture in MRS medium prevented emulsification of bile salts. Moreover, both the metabolites of TCI378 and the dead bacteria reduced oil droplet accumulation in 3T3-L1, as detected by Oil red O staining. The expressions of adipocyte-specific genes perilipin 1 and glucose transporter type 4 were suppressed by the metabolites of TCI378, indicating TCI378 may have anti-obesity effects in adipocytes. These in vitro data show the potential of the prophylactic applications of TCI378 and its metabolites for reducing fat and lowering cholesterol.

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“…Different genera are considered in this LAB group: Aerococcus, Carnobacterium, Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Oenococcus, Pediococcus, Streptococcus, Tetragenococcus, Vagococcus, and Weissella [14]. LAB are present in different ecological niches, and many studies have established differences when considering their genetic and physiology [15]. The genus Lactobacillus is certainly the most studied genus in the LAB group, and more than 240 species have been reported to be included [16], which can be found at different localizations in the human body, including the gastrointestinal tract, as well as the urinary and genital systems.…”

Section: Limosilactobacillus Fermentummentioning

confidence: 99%

Limosilactobacillus fermentum CECT5716: Mechanisms and Therapeutic Insights

et al. 2021

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Probiotics microorganisms exert their health-associated activities through some of the following general actions: competitive exclusion, enhancement of intestinal barrier function, production of bacteriocins, improvement of altered microbiota, and modulation of the immune response. Among them, Limosilactobacillus fermentum CECT5716 has become one of the most promising probiotics and it has been described to possess potential beneficial effects on inflammatory processes and immunological alterations. Different studies, preclinical and clinical trials, have evidenced its anti-inflammatory and immunomodulatory properties and elucidated the precise mechanisms of action involved in its beneficial effects. Therefore, the aim of this review is to provide an updated overview of the effect on host health, mechanisms, and future therapeutic approaches.

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Anti-pathogenic and probiotic attributes of Lactobacillus salivarius and Lactobacillus plantarum strains isolated from feces of Algerian infants and adults

Cited by 38 publications

References 32 publications

Clusters of Lactobacillus Strains from Vegetal Origins Are Associated with Beneficial Functions: Experimental Data and Statistical Interpretations

Clusters of Lactobacillus Strains from Vegetal Origins Are Associated with Beneficial Functions: Experimental Data and Statistical Interpretations

Probiotic BSH Activity and Anti-Obesity Potential of Lactobacillus plantarum Strain TCI378 Isolated from Korean Kimchi

Limosilactobacillus fermentum CECT5716: Mechanisms and Therapeutic Insights

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