Anti-Periodontitis Effects of Dendropanax morbiferus H.Lév Leaf Extract on Ligature-Induced Periodontitis in Rats

Abstract

Periodontitis is caused by pathogens in the oral cavity. It is a chronic infectious disease that causes symptoms including gingival bleeding and tooth loss resulting from the destruction of periodontal tissues coupled with inflammation. Dendropanax morbiferus H.Lév (DM) is a natural product that exhibits various biological activities with few side effects. In this study, the potential of DM leaf hot-water extracts (DMWE) as a treatment for periodontitis was determined and its anti-oxidant and anti-inflammatory effects were evaluated. Compounds in DMWE were identified by high-performance liquid chromatography (HPLC) and nitric oxide (NO) and prostaglandin E2 (PGE2) production was measured in RAW 264.7 cells. We measured the gingival index and gingival sulcus depth, and micro-CT was performed in vivo using a ligature-induced periodontitis rat model, which is similar to human periodontitis. The DMWE-treated group exhibited a decrease in cytokine concentration and relieved the gingival index and gingival sulcus depth compared with the periodontitis-induced control group. In addition, micro-CT and histological analysis revealed that DMWE exhibited anti-inflammatory effects and improved alveolar bone loss in periodontitis-induced rats. These findings suggest that DMWE has excellent anti-oxidant and anti-inflammatory effects that protect and prevent periodontal tissue damage and tooth loss caused by the inflammatory response.

Ethnomedicinal and Phytochemical Studies of Eclipta alba (A Review)

“…HPLC analysis the highest accumulation of major compounds in red light-treated cultures, while blue light led to the optimal accumulation of stigmasterol. These findings suggest that multispectral lights can be an effective strategy for enhancing phytochemical production in Eclipta alba 73 .…”

Therapeutic Effects of Hinokitiol through Regulating the SIRT1/NOX4 against Ligature-Induced Experimental Periodontitis

“…Experimental animal models of periodontitis are necessary not only to confirm complex bacterial-host reactions that are difficult to confirm in cell models, but also to confirm the effects on tooth supporting tissues such as alveolar bone [10,11]. To induce periodontitis, placing ligatures around molars or administering periodontitis-causing bacteria such as P. gingivalis are used [10,[12][13][14]. The ligature-induced model, which physically induces periodontitis by placing ligatures around the maxillary or mandibular molars, causes excessive plaque accumulation, similar to human periodontitis, and causes significant periodontal tissue destruction and alveolar bone resorption [10,12,15].…”