Anti-photoageing and anti-melanogenesis activities of chrysin

Abstract

Context: Melanin plays an important role in preventing skin photoageing by blocking ultraviolet B (UVB). However, East Asian women prefer light and fair skin, therefore they want to keep their skin from photoageing and at the same time reduce the melanin in their skin. Chrysin is a kind of natural flavonoid with luxurious biological activities, which has a very promising effect on achieving this goal. Objective: To elucidate the effects and mechanisms of chrysin on photoageing and melanogenesis. Materials and methods: Human dermal fibroblasts (HDF) and B16 murine melanoma cells were incubated with chrysin (0-25 lM) for 48 h. Anti-photoageing activity was examined in HDF by assessment of synthesis/degradation of collagen I, antioxidative and antisenescent activities through ELISA and colorimetric method. Anti-melanogenesis activity was tested by assessment of melanin, tyrosinase (TYR), melanogenic proteins inhibition activities in B16 cells using colorimetric and ELISA method. Results: Chrysin increased collagen I secretion (50-121.54% at 6.25-25 lM) and chrysin showed anti-photoageing activity by decreasing the degradation of collagen I, repairing oxidation damage and reducing the rate of HDF senescence. Furthermore, chrysin exhibited inhibitory activities with 3.00-20.35% reduction of melanin content at 6.25-25 lM, and inhibited melanin synthesis through the inhibition of TYR activity and the suppression of melanogenic proteins (TYR, TYR-related protein-1/2 and microphthalmia-associated transcription factor) expressions. Discussion and conclusion: Chrysin may have potential for developing a functional cosmetic agent because of its anti-photoageing and anti-melanogenesis activities. ARTICLE HISTORY

Photoaging protective effects of BIOGF1K, a compound-K-rich fraction prepared from Panax ginseng

“…These findings imply that BIOGF1K might act specifically against UVB-irradiation-induced photoaging processes. Moreover, α-MSH-induced hyperpigmentation due to melanin secretion, triggered by UVB irradiation, [47] was inhibited by BIOGF1K ( Fig. 3 A), suggesting that BIOGF1K can act as an antimelanogenic agent that suppresses melanin secretion.…”

Natural and Bioinspired Phenolic Compounds as Tyrosinase Inhibitors for the Treatment of Skin Hyperpigmentation: Recent Advances

“…Apart from the direct inhibition of tyrosinase activity, the anti-melanogenic activity of resorcinol in B16F10 cells has been attributed to the inhibition of cAMP signaling and activation of p38 MAPK [179]. The same applies e.g., to moracin J [181], 4,5-dicaffeoylquinic acid [183], chrysin [187], casuarictin [178], and synthetic compounds [135,136,153,197], which have been reported to both inhibit intracellular tyrosinase activity and regulate melanogenesis-related protein expression.…”

Collagen biosynthesis stimulation and anti-melanogenesis of bambara groundnut (Vigna subterranea) extracts

“…Previously found results, the phenolic and flavonoid compound present in plants, and plant extracts inhibited cellular melanin content and tyrosinase activity in α-MSH-stimulated B 16 F 10 cells though suppressing MITF, tyrosinase, TRP1 and TRP-2 (Zhu et al. 2016 ; Chatatikun et al. 2019 ).…”