Anti-Pigmentary Effect of (-)-4-Hydroxysattabacin from the Marine-Derived Bacterium Bacillus sp.

Abstract

Bioactivity-guided isolation of a crude extract from a culture broth of Bacillus sp. has led to the isolation of (-)-4-hydroxysattabacin (1). The inhibitory effect of (-)-4-hydroxysattabacin (1) was investigated on melanogenesis in the murine melanoma cell line, B16F10, and human melanoma cell line, MNT-1, as well as a pigmented 3D-human skin model. (-)-4-Hydroxysattabacin treatment decreased melanin contents in a dose-dependent manner in α-melanocyte stimulating hormone (α-MSH)-stimulated B16F10 cells. Quantitative real time PCR (qRT–PCR) demonstrated that treatment with (-)-4-hydroxysattabacin down-regulated several melanogenic genes, including tyrosinase, tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2) while their enzymatic activities were unaffected. The anti-melanogenic effects of (-)-4-hydroxysattabacin were further demonstrated in a pigmented 3D human epidermal skin model, MelanodermTM, and manifested as whitening and regression of melanocyte activation in the tissue.

Microorganisms Associated with the Marine Sponge Scopalina hapalia: A Reservoir of Bioactive Molecules to Slow Down the Aging Process

“…Kinase and proteasome inhibitors with anticancer activity along with some anti-melanogenic compounds were reported from Bacillus strains ( Bacillus spp.) [ 93 , 94 ]. Such an example is iturin A ( 3 ) (that is well-known for its strong anti-fungal activity) which inhibited protein kinases (MAPK and Akt) as well as baceridin ( 4 ), a new proteasome inhibitor [ 95 , 96 ].…”

Marine derived tyrosinase inhibitors

“…Therefore, new, active and safe hypopigmenting agents are being investigated. Under such conditions, nature is regarded as the most important and productive resource for these researches, and previous studies demonstrate this (Kim et al, 2017).…”

Effect of 3,6‐anhydro‐l‐galactose on α‐melanocyte stimulating hormone‐induced melanogenesis in human melanocytes and a skin‐equivalent model

“…To produce l ‐AHG in this study, previously used methods were used after a slight modification . Briefly, agarose was sequentially degraded using 3 enzymes in the order of Aga16B (an endo‐type β‐agarase), Aga50D (an exo‐type β‐agarase), and Sd NABH (an α‐neoagarobiose hydrolase).…”

“…Briefly, agarose was sequentially degraded using 3 enzymes in the order of Aga16B (an endo‐type β‐agarase), Aga50D (an exo‐type β‐agarase), and Sd NABH (an α‐neoagarobiose hydrolase). These enzymes all originated from Saccharophagus degradans 2‐40 T , and their genes were cloned and expressed in Escherichia coli BL21 (DE3) . In the first enzymatic reaction step, 1% (w/v) agarose was hydrolyzed mainly into neoagarohexaose and neoagarotetraose using 8 mg of Aga16B at 45°C for 1 hour in 100 mL of 20 mM Tris‐HCl buffer (pH 7.0).…”