Anti-plasmodial activities of Combretum molle (Combretaceae) [Zwoo] seed extract in Swiss albino mice

Anato, Merkin; Ketema, Tsige

doi:10.1186/s13104-018-3424-4

Cited by 11 publications

(13 citation statements)

References 18 publications

(13 reference statements)

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“…Combretum species are widely used in traditional medicine against many infectious diseases including malaria (de Morais Lima et al, 2012). Combretum mole extract had anti-plasmodial activity against P. berghei in Swiss albino mice (Anato and Ketema, 2018). In addition, C. fragrans and C. padoides extracts revealed marked inhibition against Gram-positive bacteria such as Staphylococcus aureus, S. epidermidis as well as Enterobacter aerogenes, a Gram-negative bacterium (Fyhrquist et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Potential anti-Acanthamoeba and anti-adhesion activities of Annona muricata and Combretum trifoliatum extracts and their synergistic effects in combination with chlorhexidine against Acanthamoeba triangularis trophozoites and cysts

Mitsuwan

Sin

Keo³

et al. 2021

Heliyon

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Plants with medicinal properties have been used in the treatment of several infectious diseases, including Acanthamoeba infections. The medicinal properties of Cambodian plant extracts; Annona muricata and Combretum trifoliatum were investigated against Acanthamoeba triangularis. A total of 39 plant extracts were evaluated and, as a result, 22 extracts showed positive anti-Acanthamoeba activity. Of the 22 extracts, 9 and 4 extracts showed anti-Acanthamoeba activity against trophozoites and cysts of A. triangularis, respectively. The minimum inhibitory concentration of A. muricata and C. trifoliatum extracts against trophozoites and cysts was 500 and 1,000 μg/mL, respectively. The combination of A. muricata at 1/4ÂMIC with chlorhexidine at 1/8ÂMIC demonstrated a synergistic effect against trophozoites, but partial synergy against cysts. A 40% reduction in trophozoites and 60% of cysts adhered to the plastic surface treated with both extracts at 1/2ÂMIC were noted comparing to the control (P < 0.05). Furthermore, a reduction of 80% and 90% of trophozoites adhered to the surface was observed after pretreatment with A. muricata and C. trifoliatum extracts, respectively. A 90% of cysts adhered to the surface was decreased with pre-treatment of A. muricata at 1/2ÂMIC (P < 0.05). A 75% of trophozoites and cysts from Acanthamoeba adhered to the surface were removed after treatment with both extracts at 4ÂMIC (P < 0.05). In the model of contact lens, 1 log cells/mL of trophozoites and cysts was significantly decreased post-treatment with both extracts compared to the control. Trophozoites showed strong loss of acanthopodia and thorn-like projection pseudopodia, while cysts demonstrated retraction and folded appearance treated with both extracts when observed by SEM, which suggests the potential benefits of the medicinal plants A. muricata and C. trifoliatum as an option treatment against Acanthamoeba infections.

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Section: Discussionmentioning

confidence: 99%

Potential anti-Acanthamoeba and anti-adhesion activities of Annona muricata and Combretum trifoliatum extracts and their synergistic effects in combination with chlorhexidine against Acanthamoeba triangularis trophozoites and cysts

Mitsuwan

Sin

Keo³

et al. 2021

Heliyon

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“…In Ethiopia, different parts of C. molle are wont to treat parasitic, protozoan and other infectious diseases. 16 From the promising results of the in vitro cell culture of P. falciparum K1 on leaves, root bark, and stem bark of C. molle [19][20][21] and traditional antimalarial use of C. molle, the in vivo 22,23 This high yield of methanolic extract might be because of the high efficiency of 80% methanol in partitioning a wider polarity range of compounds than pure methanol.…”

Section: Discussionmentioning

confidence: 99%

“…The methanolic crude extract yield of C. molle leaves was 19.13% while not determined in studies done on root bark and stem bark. 22 , 23 This high yield of methanolic extract might be because of the high efficiency of 80% methanol in partitioning a wider polarity range of compounds than pure methanol.…”

Section: Discussionmentioning

confidence: 99%

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Antimalarial Activity of Methanolic Extracts and Solvent Fractions of Combretum molle Leaves in Plasmodium berghei Infected Mice

Shibeshi¹,

Enyew²,

Adinew³

et al. 2021

JEP

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Background: Malaria is an infectious disease-causing mortality and morbidity in children and adults. Our study planned to measure the antimalarial activity of Combretum molle extract in vivo assays against Plasmodium berghei in Swiss albino mice. Methods: Plasmodium berghei was inoculated to healthy mice and methanolic crude extract and solvent fractions of C. molle at 100, 200, and 400mg/kg/day were administered. Percent parasitemia inhibition, percent change in weight, temperature, packed cell volume, and mean survival time were determined. Data were analyzed using one-way ANOVA followed by the post hoc Tukey HSD test with SPSS software version 24.0 and P < 0.05 considered as statistically significant. Results: Chemosuppresive effect exerted by the methanolic crude extract ranged between 27 and 68%, chloroform fraction 52.93-67.73%, hexane fraction 23.61-44.97%, and aqueous fraction 29.48-51.13%. The curative effect of the crude extract was within the range of 25-49%, chloroform fraction 42.78-69.22%, and prophylactic effect of the crude extract was within the range of 51-76.2%% and chloroform fraction 46.57-71.96%. The utmost effect in all tests on chemosuppresive, curative, prophylactic, prevention of weight loss, temperature and packed cell volume, and an increase in mean survival time was observed at higher doses of the crude extract. Conclusion: From this study, it will be concluded that crude extract of C. molle leaves has been shown promising antimalarial activity. The findings of this study may support the normal use of leaf as a part of the plant for malaria treatment.

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“…20,21 The antiplasmodial activity of the seed (in vivo) and stem bark (in vitro) extract had also been approved pharmacologically. 22,23…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antimalarial Activity of the 80% Methanolic Stem Bark Extract of Combretum molle Against Plasmodium berghei in Mice

Mulaw

Wubetu

Dessie

et al. 2019

J Evid Based Complementary Altern Med

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Background. People are dependent on the traditional use of medicinal plants for the treatment of malaria without scientific validations. Therefore, this study aimed to evaluate the antimalarial activity of methanolic stem bark extract of Combretum molle in mice. Methods. After being infected with Plasmodium berghei, the mice were randomly divided into 5 groups (n = 5). In all cases, group I mice were treated as negative control and received 3% Tween 80; group II mice were treated with 25 mg/kg chloroquine; and groups III, IV, and V mice were treated with 100 mg/kg, 200 mg/kg, and 400 mg/kg of crude extract, respectively. Data were analyzed using one-way analysis of variance followed by Tukey’s post hoc analysis. Results. In the chemosuppressive test, the parasitic suppression effect of the crude extract was found to be significant ( P < 0.05) as compared with the negative control. In the curative experiment, the average parasitic level of those mice treated by the 3 doses of the crude extract was significantly suppressed at days 5, 6, and 7 of treatment ( P < 0.001). Besides, the crude extract had been found to have a chemoprophylactic role as it inhibited the parasite level significantly relative to the negative control ( P < 0.001). Moreover, the crude extract had preventive effects on packed cell volume reduction in the 3 tests ( P < 0.001). Conclusions. The findings of the present study has supported the folklore use of the leaves of Combretum molle in the treatment of malaria. Therefore, further fractionation and characterization of the crude extract is necessary to identify the responsible lead compound(s) responsible for antiplasmodial activity.

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Anti-plasmodial activities of Combretum molle (Combretaceae) [Zwoo] seed extract in Swiss albino mice

Cited by 11 publications

References 18 publications

Potential anti-Acanthamoeba and anti-adhesion activities of Annona muricata and Combretum trifoliatum extracts and their synergistic effects in combination with chlorhexidine against Acanthamoeba triangularis trophozoites and cysts

Potential anti-Acanthamoeba and anti-adhesion activities of Annona muricata and Combretum trifoliatum extracts and their synergistic effects in combination with chlorhexidine against Acanthamoeba triangularis trophozoites and cysts

Antimalarial Activity of Methanolic Extracts and Solvent Fractions of Combretum molle Leaves in Plasmodium berghei Infected Mice

Evaluation of Antimalarial Activity of the 80% Methanolic Stem Bark Extract of Combretum molle Against Plasmodium berghei in Mice

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