Anti-plasmodial potential of crude alkaloidal extract of three plants used in traditional medicine in India

Bapna, Saroj; Choudhary, Pallavi; Satvekar, Trupti; Ramaiya, Mira; Chowdhary, Abhay

doi:10.9790/3008-09564851

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…Our study further confirms the positive impact of P. amarus on malarial infection. This could be as a result of the presence of abundant alkaloids in the extract, as alkaloids have been reported severally to possess significant antimalarial properties [25]. P. amarus antimalarial activity has been attributed to its efficiency in stabilizing erythrocyte membrane and inhibiting haem conversion to haemozoin [23].…”

Section: Discussionmentioning

confidence: 99%

Nutriphytochemical Profiling and Changes in Cardiovascular Risk Index in Malarial Infected Mice Treated with Ethanol Root Extract of Phyllanthus Amarus

Opajobi,

Uzuegbu,

Olawale

et al. 2023

ijmst

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Purpose: The use of medicinal plants in malarial treatment has become popular in Nigeria, an endemic country, due to failing chemotherapy and high cost of antimalarial drugs. One of such commonly used herbs is Phyllanthus amarus. The antimalarial activity of P. amarus has been consistently reported, giving credence to its traditional use. Nevertheless, effects of such treatment on reducing cardiovascular risk indices is yet to be fully documented. This is imperative since certain metabolic complications like increased plasma lipids have been associated with considerable risks of fatty liver and cardiovascular diseaes in severe cases of malaria in humans. Methods: In this present study, therefore, the antiplasmodial action of the ethanol root extract of P. amarus and its ED50 dose were determined and associated cardiovascular risk indices were evaluated in BALB/c malarial infected mice, having ascertained the nutritive quality and phytochemicals of plant root. Results: Results showed that P. amarus root is abundant in alkaloid phytochemical and the roots extracts demonstrated significant antiplasmodial activity in a dose-dependent trend with optimal ED50 and maximal potency in reducing cardiovascular risk indices (Atherogenic index of plasma, AIP and heart disease risk, HDR) and fatty liver, which compared well with the values obtained from the uninfected control mice. Conclusion: The roots of P. amarus possess a wide array of nutrients and phytochemicals, with significant antimalarial activity. The roots also reduced the risks of cardiovascular diseases associated with severe malaria, in experimental mice.

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Section: Discussionmentioning

confidence: 99%

Nutriphytochemical Profiling and Changes in Cardiovascular Risk Index in Malarial Infected Mice Treated with Ethanol Root Extract of Phyllanthus Amarus

Opajobi,

Uzuegbu,

Olawale

et al. 2023

ijmst

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Evaluation of Antileukemic and Antimalarial Potential of Zanthoxylum rhetsa DC (Rutaceae), A Well-known Spice

Mallya

Bhitre

2022

CDTH

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Introduction: Zanthoxylum rhetsa fruits, a common spice in many cuisines, have proven to have a good therapeutic potential and are routinely used in food, medicine, and commerce. The present study was conducted to screen the in vitro antileukemic and antimalarial activities of the methanolic extract of Z. rhetsa fruits and conduct mechanistic studies for antileukemic activity. Methods: Methanol extract was prepared by maceration process and standardised with lupeol as a marker using HPLC. MTT and SRB assays were used to establish the cytotoxicity of the extract against L929 and leukemic cell lines (Jurkat, K562, and HL-60). The amount of ROS in cell lines was detected by flow cytometry using 2',7'-dichlorodihydrofluorescin diacetate. Apoptosis on HL-60 was detected by Annexin-V/PI dual staining assay through cell cycle analysis and gel electrophoresis. In vitro antimalarial activity was conducted on Plasmodium falciparum CQ sensitive 3D7 strains according to the WHO 2001 guidelines. Results: The methanol extract contained 1.03% of lupeol. Potent antileukemic activity (IC50 <10 μg/mL) was observed against HL-60 in comparison to K562 and Jurkat cell lines. The extract induced apoptosis in cancer cells in the proliferative and mitotic phase without DNA fragmentation. Therefore, the antileukemic activity exhibited by the extract could be attributed to the increased oxidative stress generated in cancer cells. Fruits also exhibited good antioxidant activity against normal cells, thus proving beneficial as cytoprotective agents. Promising antimalarial activity (IC50 = 16.21 μg/mL) with high selectivity against malarial parasites was exhibited by the fruits. Conclusion: Thus, the fruits of Z.rhetsa can be used as an adjuvant therapy to reduce the side effects and resistance associated with chemotherapy and can be a potential candidate for drug discovery research in the areas of cancer and parasitic infection.

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Anti-plasmodial potential of crude alkaloidal extract of three plants used in traditional medicine in India

Cited by 2 publications

References 19 publications

Nutriphytochemical Profiling and Changes in Cardiovascular Risk Index in Malarial Infected Mice Treated with Ethanol Root Extract of Phyllanthus Amarus

Nutriphytochemical Profiling and Changes in Cardiovascular Risk Index in Malarial Infected Mice Treated with Ethanol Root Extract of Phyllanthus Amarus

Evaluation of Antileukemic and Antimalarial Potential of Zanthoxylum rhetsa DC (Rutaceae), A Well-known Spice

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