Anti-platelet aggregation of mixtures of betulinic oleanolic and maslinic acids and derivatives from medicinal plants

Osunsanmi, Foluso Oluwagbemiga; Oyinloye, Babatunji Emmanuel; Mosa, Rebamang A.; Ikhile, Monisola I.; Ngila, Jane Catherine; Shode, Francis O.; Singh, Moganavelli; Opoku, Andy R.

doi:10.4314/tjpr.v15i8.3

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…61 Moreover, these findings correlated with previous studies such as that of Osunsanmi et al , who reported that triterpenes have anti-platelet aggregation properties through modifying hydroxyl moiety at the carbon-3 position with the acetyl group, and prevent platelet activation. 62 The decrease in platelet activation may prevent the pathogenesis of cardiovascular complications in pre-diabetes, which is reported mostly in people with diabetes and metabolic syndrome. In addition, treatment with MET both in the presence and in the absence of dietary intervention yielded similar results, as expected.…”

Section: Discussionmentioning

confidence: 99%

Plant-derived oleanolic acid ameliorates markers of subclinical inflammation and innate immunity activation in diet-induced pre-diabetic rats

Gamede

Mabuza

Ngubane

et al. 2020

Therapeutic Advances in Endocrinology

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Aims: Sub-clinical inflammation during pre-diabetes is one of the predisposing factors that facilitates the progression of pre-diabetes to type 2 diabetes. The administration of oleanolic acid (OA) with or without dietary intervention ameliorates the metabolic and cardiovascular complications in diet-induced pre-diabetes animal models of pre-diabetes. This study aimed to investigate whether OA can also suppress immune activation and ameliorate pro-inflammatory markers. Methods: Pre-diabetes was induced by feeding Sprague Dawley rats a high-fat high carbohydrate diet for 20 weeks. The pre-diabetic rats were then treated with OA (80 mg/kg) or metformin (500 mg/kg) in the presence or absence of dietary interventions for a period of 12 weeks. At the end of the treatment period, the animals were euthanised and whole blood was used for platelet and immune cell count while plasma was used for fibrinogen, cluster differentiation 40 ligand and pro-inflammatory cytokine evaluation. Results: The results of this study revealed that OA, with or without dietary intervention, improved lipid metabolism by restoring high-density lipoprotein (HDL) and low-density lipoproteins (LDLs) as well as reducing platelets and immune cell counts. Furthermore, OA also decreased plasma proinflammatory cytokines, including tumour necrosis factor-α and -1β. Markers of immune activation such as C-reactive protein, fibrinogen, and CD40L were also decreased upon administration of OA with or without dietary intervention. Conclusion: The findings of this study suggest that OA may provide an alternative to prevent the progression of pre-diabetes to overt diabetes. This was evident by the reduction of differential white blood cell count and proinflammatory cytokines that exercebate insulin resistance. However, more studies are needed to elucidate the molecular mechanisms and to improve efficacy.

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Section: Discussionmentioning

confidence: 99%

Plant-derived oleanolic acid ameliorates markers of subclinical inflammation and innate immunity activation in diet-induced pre-diabetic rats

Gamede

Mabuza

Ngubane

et al. 2020

Therapeutic Advances in Endocrinology

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“…High phenolic content was reported to show positive correction with scavenging ability. 29 Phenolic compound easily loss electron due to its hydroxyl moiety, thus reduce free radical activity. 26 Minimum inhibition concentration at 50 % (IC 50 ) is defined as the minimum concentration of compound or extract to inhibit particular activity.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Constituents and Antioxidant Activities of Crude Extracts from Acacia Senegal Leaf Extracts

Uzunuigbe¹,

Osunsanmi²,

Masamba³

et al. 2019

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Background: Acacia senegal (Fabaceae) Wild is a leguminous tree with economic values, but its leaves are under-utilised. Objective: To investigate the phytochemical constituents and antioxidant potential of crude extracts from A. Senegal's leaves. Methods: Methanol and acetone crude extracts of leaves of A. senegal were prepared by maceration using organic solvents, methanol and acetone respectively. Qualitative and quantitative phytochemical analysis of the crude extracts were evaluated using Association of Agricultural and Chemist (AOAC) protocols. Antioxidant activities of the crude extracts were determined using 2, 2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) respectively. Results: The crude extracts (acetone and methanol) showed vary quality of phytochemical constituent including flavonoid, alkaloids, carbohydrate, saponins, tannin, steroids, and terpenoids. Acetone crude possessed significant (P < 0.05) higher total flavonoid and proanthocyanidin content in comparison with methanol extracts. Whereas, methanol crude extract possessed significant higher total phenol content compared with acetone crude extract. The crude extracts showed antioxidant activities as evidence in scavenging ABTS and DPPH radicals. However, acetone crude with lower IC 50 of 0.09 mg/mL possessed significant higher ABTS scavenging ability compared to methanol (0.07 mg/mL) and ascorbic acid (0.07 mg/mL). Conclusion: The crude extracts could serve as a promising natural antioxidant agent in management of oxidative stress diseases. For further studies, bioactive compounds need to be ascertained.

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“…Previous studies on different extracts of M. bracteata var. Revolution Gold revealed the isolation of betulinic acid, oleanolic acid, maslinic acid and their derivatives, with many possessing antibacterial, anti-inflammatory, antiplatelet aggregation, antifungal, antiulcer antioxidant, anti-sickling and cytotoxic activities [3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Melaleuca bracteata var. Revolution Gold (Myrtaceae) Essential Oil: Chemical Composition, Antibacterial, Membrane Damage, Antiplatelet Aggregation and Antiacetylcholinesterase Activities

A. Lawal,

O. Amisu,

A. Mosa

et al. 2024

Medicinal Plants - Chemical, Biochemical, and Pharmacological Approaches

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Melaleuca bracteata var. Revolution Gold (a cultivar of Melaleuca bracteata) is an ornamental plant, which has been used in traditional medicine for the treatment of several diseases. Till moment, information is scanty on the biological activities of the essential oil from the plant. The water-distilled essential oil was analyzed by gas chromatography and gas chromatography-mass spectrometry. Antibacterial activity of the oil was evaluated by paper disc diffusion and micro-dilution methods. Cell membrane damage was assay using cytosolic lactate dehydrogenase released method. Platelet aggregation inhibitory activity was separately evaluated on Adenosine diphosphate, collagen, epinephrine and thrombin induced aggregation. Thirteen components representing 95.3% of the total oil were identified from the essential oil. Phenylpropanoids (82.9%) constitute the predominant class of compounds in the oil. On the whole, the oil displayed strong antibacterial action towards Staphylococcus aureus, moderate activity on Bacillus cereus and some strains of Escherichia coli. The lactate dehydrogenase released (0.78–47%) depicted the oil to exhibit low levels of membrane damage. The percentage platelet aggregation inhibition for the four platelet agonists was concentration dependent with thrombin > collagen > ADP > epi-nephrine. The acetylcholinesterase inhibitory activity (9.16%) indicated that the essential oil was not effective against the enzyme.

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Anti-platelet aggregation of mixtures of betulinic oleanolic and maslinic acids and derivatives from medicinal plants

Abstract: HEK293 cells (IC50: 724.43, 269.08 and 407.89 mg/mL respectively) and HEPG2 (IC50: 585.38, 499.78 and 499.78 mg/mL, respectively

Cited by 4 publications

References 17 publications

Plant-derived oleanolic acid ameliorates markers of subclinical inflammation and innate immunity activation in diet-induced pre-diabetic rats

Plant-derived oleanolic acid ameliorates markers of subclinical inflammation and innate immunity activation in diet-induced pre-diabetic rats

Phytochemical Constituents and Antioxidant Activities of Crude Extracts from Acacia Senegal Leaf Extracts

Melaleuca bracteata var. Revolution Gold (Myrtaceae) Essential Oil: Chemical Composition, Antibacterial, Membrane Damage, Antiplatelet Aggregation and Antiacetylcholinesterase Activities

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