Anti-platelet pentacyclic triterpenoids from leaves ofcampsis grandiflora

Abstract

Five pentacyclic triterpenoids, oleanolic acid (1), hederagenin (2), ursolic acid (3), tormentic acid (4) and myrianthic acid (5), were isolated from the methanol extract of the leaves of Campsis grandiflora, and structures of the compounds were established by the spectroscopic methods. Compounds 2, 3, 4, and 5 were isolated for the first time from the genus Campsis. All of the compounds (IC50: 45.3, 32.8, 82.6, 42.9 and 46.2 microM respectively) were as equivalently inhibitive as acetylsalicylic acid (IC50: 57.0 microM) on epinephrine induced platelet aggregation.

24-nor-Ursane Type Triterpenoids from the Stems of Rumex japonicus

“…10) The 2a,3a-configurations of the two secondary hydroxyl groups of 1 were in agreement with the similarity of the observed coupling constant (Jϭ2.4 Hz) between the H2b and H-3b proton and those for myrianthic acid (3, Jϭ2.7 Hz), 9) which was also isolated during the present investigation. Moreover, the chemical shifts of the C-1-C-5, C-10, C-23, and C-25 carbons of 1 were identical to those reported for 2a,3a-dihydroxy-24-nor-4(23),12-oleanadien-28-oic acid, 11) a triterpenoid previously isolated from Salvia carduacea.…”

“…4,5) Although previous phytochemical investigations of the roots and leaves of R. japonicus have resulted in the isolation of several flavonoids, 6) anthraquinone derivatives, 3,7) and naphthalene derivatives, 3,4,6,8) no phytochemical studies have been carried out to date on the stems of this plant. In the present paper we report the isolation and structure elucidation of two new 24-norursane type triterpenoids (1, 2) and the known compounds myrianthic acid (3), 9) tormentic acid, 9) and emodin 7) from an EtOAc-soluble fraction of the stems of R. japonicus. Fractionation of an EtOAc-soluble extract of the stems of Rumex japonicus led to the isolation of two new 24-norursane type triterpenoids, 2a a,3a a,19a a-trihydroxy-24-norurs-4(23),12-dien-28-oic acid (1) and 4(R),23-epoxy-2a a,3a a,19a a-trihydroxy-24-norurs-12-en-28-oic acid (2), along with the known compounds myrianthic acid (3), tormentic acid, and emodin.…”

“…The known compounds were identified as myrianthic acid (3), 9) tormentic acid, 9) and emodin 7) by physical and spectroscopic data ([a] D , mp, …”

Chemical Constituents, Antischistosomal and Antioxidant Activities of the Methanolic Extract of Azadirachta indica

“…Consequently, compound 3 was identified to be quercetin-3-O-α-L-rhamnopyranoside. ) (34,35) . It gave positive reactions of triterpenoidal saponins (36) .…”

Antidiabetic and antiplatelet aggregation study of various methanol fractions of Nymphaea stellata Willd. leaves

“…6). The exhibited activity of UPFME can be attributed to the antiplatelet aggregation activity of oleanolic acid [31] and gallic acid [32]. CFME and RFME increased aggregation of platelets.…”