Anti/pro-oxidative properties of selected standard chemicals and tea extracts investigated by DNA-based electrochemical biosensor

Ferancová, Adriana; Heilerová, Ľudmila; Korgová, Elena; Šilhár, S.; Stĕpánek, I; Labuda, Ján

doi:10.1007/s00217-004-1001-7

Cited by 34 publications

(11 citation statements)

References 28 publications

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“…When cyclic voltammetry is used, it is often compared either to some spectrophotometric [11][12][13][14] or the HPLC method [6,7,15]. Comparison of CV to the spectrophotometric methods based on the reaction with free radicals is particularly interesting because redox behavior of an antioxidant at an electrode is correlated to its behavior in a chemical redox reaction with the radical.…”

Section: Introductionmentioning

confidence: 99%

Cyclic voltammetry study of plasma antioxidant capacity – Comparison with the DPPH and TAS spectrophotometric methods

Martinez

Valek

Rešetić

et al. 2006

Journal of Electroanalytical Chemistry

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Section: Introductionmentioning

confidence: 99%

Cyclic voltammetry study of plasma antioxidant capacity – Comparison with the DPPH and TAS spectrophotometric methods

Martinez

Valek

Rešetić

et al. 2006

Journal of Electroanalytical Chemistry

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“…Ag-NPs have free electrons, which gives a rise into the SPR absorption band [31], due to the combined vibration of electrons of metal nanoparticles in resonance with the light wave [32]. A SPR spectra for Ag-NPs were obtained at 443 nm.…”

Section: Characterization By Uv-vis and Ft-ir Of The Synthesized "Ultmentioning

confidence: 99%

“…Over the past years, flavonoids have gained much interest because of their protective effects on DNA damage [29], anti-inflammatory [30] and their free radical scavenging activities [31]; furthermore, they can protect against cardiovascular diseases [32] and certain kinds of cancers [33,34]. In this study a simple and low-cost green synthesis of Ag-NPs using petals extract of Rosa 'Andeli' (PERA) double delight hybrid tea rose, has been reported.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, kinetics and photocatalytic study of “ultra-small” Ag-NPs obtained by a green chemistry method using an extract of Rosa ‘Andeli’ double delight petals

Suárez-Cerda

Alonso-Núñez

Espinoza-Gómez³

et al. 2015

Journal of Colloid and Interface Science

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“…On the other hand, disposable sensors based on the SPE may be advantageous in practical analysis [29,38,40]. We therefore tested if the SPE sensors can be used in the SS enzyme-linked RP hybridization assay instead of the PGE.…”

Section: Measurements With Screen-printed Electrodesmentioning

confidence: 99%

A Single-Surface Electrochemical Biosensor for the Detection of DNA Triplet Repeat Expansion

et al. 2006

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Molecular diagnostics of inherited neurodegenerative disorders such as fragile X syndrome, myotonic dystrophy or Friedreich ataxia (FRDA) is based on analysis of the length of trinucleotide repetitive sequences in certain loci of genomic DNA. The current methods employ PCR and electrophoretic determination of the amplified DNA fragment size. We have recently shown that length of a triplet repetitive DNA sequence can be determined using a doublesurface electrochemical technique involving multiple hybridization of the expanded triplet repeat with short labeled reporter probe (spanning several trinucleotides). Here we propose a single-surface sensor employing an analogous principle. Target DNA (tDNA) is adsorbed onto surface of a carbon (pyrolytic graphite or screen-printed) electrode. Biotin-labeled reporter probe (RP) is hybridized with the immobilized tDNA followed by binding of streptavidinalkaline phosphatase (ALP) conjugate. The ALP catalyzes production of an electroactive indicator (1-naphthol) which is detected voltammetrically on the same electrode. Signal resulting from this electrochemical enzyme-linked DNA hybridization assay is normalized to the amount of tDNA immobilized at the transducer surface either by measuring intrinsic tDNA voltammetric response, or using electrochemical labeling of the tDNA with osmium tetroxide 2,2'-bipyridine complex. Detection of (GAA) n · (TTC) n triplet repeat expansion in nanogram quantities of PCR-amplified tDNAs, including amplicons of patients genomic DNA, is demonstrated. We show that our technique allow differentiation between normal and pathological alleles of X25 gene related to the FRDA.

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Anti/pro-oxidative properties of selected standard chemicals and tea extracts investigated by DNA-based electrochemical biosensor

Cited by 34 publications

References 28 publications

Cyclic voltammetry study of plasma antioxidant capacity – Comparison with the DPPH and TAS spectrophotometric methods

Cyclic voltammetry study of plasma antioxidant capacity – Comparison with the DPPH and TAS spectrophotometric methods

Synthesis, kinetics and photocatalytic study of “ultra-small” Ag-NPs obtained by a green chemistry method using an extract of Rosa ‘Andeli’ double delight petals

A Single-Surface Electrochemical Biosensor for the Detection of DNA Triplet Repeat Expansion

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