Anti-proliferation activities of three bioactive components purified by high-speed counter-current chromatography in essential oil from ginger

Wang, Chenxu; Wang, Lixia; Li, Chi‐Yu; Hu, Chen; Zhao, Shou‐Xun

doi:10.1007/s00217-020-03446-7

Cited by 17 publications

(10 citation statements)

References 40 publications

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“…The yield of 6-gingerol, 8-gingerol, and 10 gingerol from 360 mg of pre-purified samples were 132 mg (purity; 98.7%), 31 mg (purity; 99.3%), and 61 mg (purity; 98.5%), respectively (Wang et al, 2011). Wang et al (2020) performed HSCCC using different two-phase solvent systems at a flow rate of 2 mL/min and 35 mg of 6-gingerol was obtained with 98.6% purity. In another study, Gan et al (2016) found that a two-phase solvent system composed of n-hexane/ ethyl acetate/methanol/water (10/2/5/7, v/v/v/v) was optimal for the isolation of 6-gingerol by HSCCC.…”

Section: High-speed Counter-current Chromatographymentioning

confidence: 99%

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Extraction, purification, food applications, and recent advances for enhancing the bioavailability of 6-gingerol from ginger – A review

Arachchilage

Young

Choo

2022

Qual. Assur. Saf. Crops Foods

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6-Gingerol is the major pharmacologically active component of ginger (Zingiber officinale) rhizome widely used in the food, cosmetics, and pharmaceutical industries. Various extraction and purification methods have been devel-oped to obtain highly purified 6-gingerol. 6-Gingerol can be extracted using conventional and nonconventional extraction techniques. Hydroalcoholic solutions and liquid CO2 are the most suitable solvents for the extraction of 6-gingerol, while microwave-assisted extraction is the best extraction method. High-speed counter-current chro-matography is the purification technique resulting in the highest purity of 6-gingerol. Despite the various biological properties of 6-gingerol, the low bioavailability of 6-gingerol is the main challenge that limits its application. Novel encapsulation and solubilization techniques, including nanoemulsion, complexation, micelles, and solid dispersion methods, have been introduced to enhance the bioavailability of 6-gingerol, overcoming its limitations. 6-Gingerol showed potential applications as a natural antioxidant, preservative, and flavor enhancer as well as demonstrated a synergistic effect with different ingredients for maintaining the quality and shelf-life of the food products. The current work provides a comprehensive review of the prevailing techniques applied for extraction and purification of 6-gingerol from the rhizome of ginger, current research on the application of 6-gingerol in the food industry, and novel advances for increasing its bioavailability.

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Section: High-speed Counter-current Chromatographymentioning

confidence: 99%

“…HSCCC is a partition chromatography technique based on the separation of compounds using two immiscible liquid phases, whereby the stationary phase is a liquid (Wang et al, 2020). HSCCC has been extensively used to isolate and purify active compounds from natural products (Chen et al, 2018).…”

Section: High-speed Counter-current Chromatographymentioning

confidence: 99%

Extraction, purification, food applications, and recent advances for enhancing the bioavailability of 6-gingerol from ginger – A review

Arachchilage

Young

Choo

2022

Qual. Assur. Saf. Crops Foods

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“…The remaining part of ginger after extraction of essential oil is usually ignored, which leads to a deficient utilization of ginger. A two-phase solvent system HSCCC had been implemented as an effective method to isolate and purify gingerols from ginger or ginger essential oil in previous researches (Wang et al 2020(Wang et al , 2011Zhan et al 2011). The RGP has not been analyzed and utilized in detail.…”

Section: Introductionmentioning

confidence: 99%

Identification of Five Flavonoid Compounds from the Remaining Ginger Powder Purified by Using High-Speed Counter-Current Chromatography and Their Bioactivity

Wang

Zhang

et al. 2021

Food Anal. Methods

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HSCCC was widely used as a reliable and efficient tool for isolation of natural production. Multiple active compounds were separated and purified from ginger essential oil. But the remaining ginger powder (RGP), which was obtained from ginger without volatile oil, was discarded commonly. The crude flavonoids were extracted from RGP using ethanol at 80°C. The purification of flavonoids was performed by rotary evaporator and X-5 macroporous adsorption resin. The high-speed counter-current chromatography (HSCCC) was implemented by employing a two-phase solvent system which consist of n-hexane-ethyl acetate-methanol-water (4:5:4:5, v/v/v/v) and n-hexane-ethyl acetate-methanol-water (1:5:1:5, v/v/v/v) to separate flavonoid into five fractions. The purity of individual flavonoid in each of the fraction was analyzed by highperformance liquid chromatography (HPLC). The chemical structures of the isolated compounds were identified by liquid chromatograph mass spectrometry-ion trap-time of flight (LCMS-IT-TOF). Rutin (16.6%), tangeretin (13.1%), vitexin (18.37%), myricetin-3-O-glucopyranoside (3.3%), and isoquercitrin (15.2%) were identified in RGP at the purity of all over 90%. The HSCCC appeared to be an efficient and reliable method for the isolation of flavonoids in RGP. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to determine the anti-tumor abilities of five flavonoid compounds. The results showed that rutin and myricetin-3-O-glucopyranoside had better anti-tumor activities than other three flavonoid compounds from RGP. The research revealed that RGP was a bioactive product and contributed to development of ginger. KeywordsRemaining ginger powder • High-speed counter-current chromatography • High-performance liquid chromatography • Liquid chromatograph mass spectrometry-ion trap-time of flight • Anti-tumor activity * Li-xia Wang

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“…[ 25 , 26 ]. Compared with conventional column chromatography, high-speed counter-current chromatography (HSCCC) is a continuous and efficient liquid-liquid extractive chromatography with no solid support [ 27 , 28 , 29 ], which can also eliminate the irreversible adsorption problem of the sample and separate different substances from the two phases through different distribution coefficients [ 30 , 31 ]. HSCCC has the advantages of recoverable samples, less solvent consumption, and low cost [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Combined Ultrahigh Pressure Extraction and High-Speed Counter-Current Chromatography for Separation and Purification of Three Glycoside Compounds from Dendrobium officinale Protocorm

Zhang

Wang

et al. 2021

Molecules

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As an alternative to Dendrobium candidum, protocorm-like bodies (PLBs) of Dendrobium candidum are of great value due to their high yield and low cost. In this work, three glycoside compounds, β-D-glucopyranose 1-[(E)-3-(4-hydroxyphenyl)-2-propenoat] (I), β-D-glucopyranose 1-[(E)-3-(3, 4-dihydroxyphenyl)-2-propenoat] (II), and 1-O-sinapoyl glucopyranoside (III), were extracted and isolated by ultrahigh pressure extraction (UPE) coupled with high-speed counter-current chromatography (HSCCC) from PLBs of D. officinale. First, the target compounds were optimized and prepared with 50% ethanol solution at a 1:30 (g/mL) solid/liquid ratio in 2 min under 300 MPa by UPE. Then, the crude extract was chromatographed with a silica gel column, and primary separation products were obtained. In addition, the products (150 mg) were separated by HSCCC under the solvent system of MTBE-n-butyl alcohol-acetonitrile-water (5:1:2:6, v/v/v/v), yielding 31.43 mg of compound I, 10.21 mg of compound II, and 24.75 mg of compound III. Their structures were further identified by ESI-MS, 1H NMR, and 13C NMR. The antioxidant results showed that the three compounds expressed moderate effects on the DPPH· scavenging effect. Compound II had the best antioxidant capacity and its IC50 value was 0.0497 mg/mL.

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Anti-proliferation activities of three bioactive components purified by high-speed counter-current chromatography in essential oil from ginger

Cited by 17 publications

References 40 publications

Extraction, purification, food applications, and recent advances for enhancing the bioavailability of 6-gingerol from ginger – A review

Extraction, purification, food applications, and recent advances for enhancing the bioavailability of 6-gingerol from ginger – A review

Identification of Five Flavonoid Compounds from the Remaining Ginger Powder Purified by Using High-Speed Counter-Current Chromatography and Their Bioactivity

Combined Ultrahigh Pressure Extraction and High-Speed Counter-Current Chromatography for Separation and Purification of Three Glycoside Compounds from Dendrobium officinale Protocorm

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