Anti-proliferation and induction of mitochondria-mediated apoptosis by Garcinia hanburyi resin in colorectal cancer cells

Vichitsakul, Kanokkorn; Laowichuwakonnukul, Khanittha; Soontornworajit, Boonchoy; Poomipark, Natwadee; Itharat, Arunporn; Rotkrua, Pichayanoot

doi:10.1016/j.heliyon.2023.e16411

Cited by 12 publications

(4 citation statements)

References 32 publications

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“…Values presented are mean ± standard error of the mean. The SI of each compound was calculated as GI 50 of the extract against the CCD-18 normal cell line/GI 50 of the same extract against the HT-29 cancer cell line [14].…”

Section: Cell Assays On Cancer and Normal Cell Linesmentioning

confidence: 99%

“…Finally, the selectivity index (SI) of HT-29 versus normal cells CCD-18 was calculated (see Material and Methods, Section 2). An extract with an SI value greater than two is considered as high selectivity against cancer cells, whereas one with an SI value less than two demonstrates general toxicity to normal cells [14]. Such value for any research on herbal drugs and/or isolated compounds is critical for determining whether further research can be continued [35].…”

Section: Antiproliferative Activity Of the Water/methanol Seed Extrac...mentioning

confidence: 99%

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Phenolic Profiles and Antitumor Activity against Colorectal Cancer Cells of Seeds from Selected Ribes Taxa

Lyashenko,

López-Ruiz,

García-Cervantes

et al. 2024

Applied Sciences

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Seeds from several Ribes taxa were surveyed for phenolic compounds and in vitro antiproliferative activity against HT-29 colorectal cancer cells. Total phenolic compounds were analyzed through the Folin–Ciocalteu procedure, while LC coupled to a single mass spectrometer (MS) Orbitrap using an electrospray interface (ESI) was performed to determine the phenolic profiles. Antitumor effects were established using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Total phenolics ranged from 11.4 in R. alpinum to 94.8 mg of caffeic acid equivalents (CAE)/g in R. nigrum ‘Koksa’. Concerning phenolic compounds, four were hydroxylated benzoic acids, four cinnamic acid derivatives, eight flavonoids, and nine flavonoid glycosides. The growth inhibition against HT-29 cancer cells was exercised much better by R. nigrum ‘Koksa’ and Ribes ‘Erkeeni’ (GI50 37 and 42 µg/mL). All Ribes extracts, except for R. nigrum ‘Hara katarlik’, showed higher activity than R. rubrum (GI50 at 72 h: 99 µg/mL). Interestingly, the extract from Ribes ‘Erkeeni’, which exhibited high bioactivity, contains all detected phenolic compounds, unlike R. nigrum ‘Koksa’, which lacks only populnin. Therefore, the high bioactivity found for such extracts could be due to a synergy of all detected compounds. This work constitutes a comprehensive action for expanding knowledge on the phenolic profiles and antitumor activity of GLA-rich Ribes seeds.

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Section: Cell Assays On Cancer and Normal Cell Linesmentioning

confidence: 99%

Section: Antiproliferative Activity Of the Water/methanol Seed Extrac...mentioning

confidence: 99%

Phenolic Profiles and Antitumor Activity against Colorectal Cancer Cells of Seeds from Selected Ribes Taxa

Lyashenko,

López-Ruiz,

García-Cervantes

et al. 2024

Applied Sciences

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“…In recent years, this resin has garnered heightened attention due to its broad spectrum of biological and pharmacological properties. These properties encompass cytotoxic [7][8][9], anti-proliferative [10][11][12], antitumor [13,14], anti-angiogenesis [15], anti-HIV-1 [16,17], antibacterial [18], and anti-inflammatory effects [19]. Gamboge contains a diverse array of bioactive components, primarily caged xanthones.…”

Section: Introductionmentioning

confidence: 99%

Unveiling Gambogenic Acid as a Promising Antitumor Compound: A Review

Mi,

Xing,

Zhang

et al. 2024

Planta Med

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Gambogenic acid is a derivative of gambogic acid, a polyprenylated xanthone isolated from Garcinia hanburyi. Compared with the more widely studied gambogic acid, gambogenic acid has demonstrated advantages such as a more potent antitumor effect and less systemic toxicity than gambogic acid according to early investigations. Therefore, the present review summarizes the effectiveness and mechanisms of gambogenic acid in different cancers and highlights the mechanisms of action. In addition, drug delivery systems to improve the bioavailability of gambogenic acid and its pharmacokinetic profile are included. Gambogenic acid has been applied to treat a wide range of cancers, such as lung, liver, colorectal, breast, gastric, bladder, and prostate cancers. Gambogenic acid exerts its antitumor effects as a novel class of enhancer of zeste homolog 2 inhibitors. It prevents cancer cell proliferation by inducing apoptosis, ferroptosis, and necroptosis and controlling the cell cycle as well as autophagy. Gambogenic acid also hinders tumor cell invasion and metastasis by downregulating metastasis-related proteins. Moreover, gambogenic acid increases the sensitivity of cancer cells to chemotherapy and has shown effects on multidrug resistance in malignancy. This review adds insights for the prevention and treatment of cancers using gambogenic acid.

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“…Colorectal cancer (CRC) is one of the most frequent cancers [4] and is responsible for highest mortality rate in developing countries in both sexes [5]. Despite CRC's current therapy regimens such as chemotherapy, radiation therapy, and surgery, these regimens have several limitations, e.g., cost, drug resistance, intra-tumor heterogeneity, tumor evolution, and other adverse effects [6]. Currently, new antitumor treatments like organ transplantation, targeted therapy, and immunotherapy [7] have been drawing attention as CRC treatment options; however, these too have adverse effects, leading to early mortality risk and drug discontinuation [8,9].…”

Section: Introductionmentioning

confidence: 99%

Antitumor and Phytochemical Properties of Ferula assa-foetida L. Oleo-Gum–Resin against HT-29 Colorectal Cancer Cells In Vitro and in a Xenograft Mouse Model

Elarabany,

Hamad,

Alzamel

2023

Molecules

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Colorectal cancer (CRC) is one of the most frequently occurring tumors. Ferula assa-foetida oleo-gum–resin (OGR) extract is a traditional cooking spice known for its broad spectrum of biological activities such as antifungal, antiparasitic, and anti-inflammatory activities. This study evaluated the antitumor effect of OGR extract against HT-29 colorectal cancer cells. The OGR chemical composition was analyzed using LC–ESI–MS/MS; MTT, clonogenic assays, and a xenograft model were used to measure cytotoxicity, while apoptotic proteins were detected using Western blotting. Phytochemical analysis revealed that the extract was a rich source of isoflavones, xanthones, and other derivatives. In a dose-dependent manner, the OGR extract significantly inhibited colony formation ability and HT-29 cell growth (IC50 was 3.60 ± 0.02 and 10.5 ± 0.1 mg/mL, respectively). On the other hand, the OGR extract significantly induced apoptosis and increased the expression of some pro-death proteins involved in cellular apoptosis including PUMA, BIM, BIK, and BAK. Moreover, in a subcutaneous HT-29 xenograft model, the tumor volume and burden decreased after treatment with the OGR extract (550 ± 32 mm3 and 16.3 ± 3.6, respectively) This study demonstrated that Ferula assa-foetida OGR ethanolic extract has potential antitumor effects against HT-29 CRC cell lines by reducing cell viability and the function of apoptosis. More studies are needed to reveal the underlying mechanisms related to cytotoxicity and apoptosis induction.

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Anti-proliferation and induction of mitochondria-mediated apoptosis by Garcinia hanburyi resin in colorectal cancer cells

Cited by 12 publications

References 32 publications

Phenolic Profiles and Antitumor Activity against Colorectal Cancer Cells of Seeds from Selected Ribes Taxa

Phenolic Profiles and Antitumor Activity against Colorectal Cancer Cells of Seeds from Selected Ribes Taxa

Unveiling Gambogenic Acid as a Promising Antitumor Compound: A Review

Antitumor and Phytochemical Properties of Ferula assa-foetida L. Oleo-Gum–Resin against HT-29 Colorectal Cancer Cells In Vitro and in a Xenograft Mouse Model

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