Anti-proliferation Effect of Polypeptide Extracted from Scorpion Venom on Human Prostate Cancer Cells in vitro

Zhang, Yue Ying; Wu, Langping; Wang, Zhao Peng; Jia, Qiong; Jiang, Guo Sheng; Zhang, Wensheng

doi:10.4021/jocmr2009.01.1220

Cited by 28 publications

(26 citation statements)

References 37 publications

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“…These results agree with previous reports that scorpion venom inhibited the growth of lymphoma (3,9), leukemia (10), neuroblastoma (4), gliomas (11)(12)(13), breast cancer (14) and prostate cancer (15,16).…”

Section: Discussionsupporting

confidence: 93%

Scorpion venom component III inhibits cell proliferation by modulating NF-κB activation in human leukemia cells

Song

Zhang

Sun

et al. 2012

Experimental and Therapeutic Medicine

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Abstract. Scorpion venom contains various groups of compounds that exhibit anticancer activity against a variety of malignancies through a poorly understood mechanism. While the aberrant activation of nuclear factor κB (NF-κB) has been linked with hematopoietic malignancies, we hypothesized that scorpion venom mediates its effects by modulating the NF-κB signaling pathway. In the present study, we examined the effects of scorpion venom component III (SVCIII) on the human leukemia cell lines THP-1 and Jurkat and focused on the NF-κB signaling pathway. Our results showed that SVCIII inhibited cell proliferation, caused cell cycle arrest at G1 phase and inhibited the expression of cell cycle regulatory protein cyclin D1 in a dose-dependent manner in THP-1 and Jurkat cells. SVCIII also suppressed the constitutive NF-κB activation through inhibition of the phosphorylation and degradation of IκBα. NF-κB luciferase reporter activity was also inhibited by SVCIII. Our data suggest that SVCIII, a natural compound, may exert its antiproliferative effects by inhibiting the activation of NF-κB and, thus, has potential use in the treatment of hematopoietic malignancies, alone or in combination with other agents.

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Section: Discussionsupporting

confidence: 93%

Scorpion venom component III inhibits cell proliferation by modulating NF-κB activation in human leukemia cells

Song

Zhang

Sun

et al. 2012

Experimental and Therapeutic Medicine

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“…Ki-67 is a nuclear protein expressed in proliferating cells (in all phases of the cell cycle except G0) and serves as a good marker for proliferation and may be required for maintaining cell proliferation (Schluter et al 1993 ; Miller et al 1994 ). Venoms from various scorpions have been reported to prevent propagation of different cell lines such as prostate cancer, human leukemia and neuroblastoma (Gupta et al 2007 ; Zhang et al 2009 ; Zargan et al 2011a ). For example, venom of A. crassicauda inhibited proliferation of human neuroblastoma cell lines through arresting S-phase and induction of apoptosis (Zargan et al 2011a ).…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo antitumor effects of the Egyptian scorpion Androctonus amoreuxi venom in an Ehrlich ascites tumor model

et al. 2016

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Scorpion venom is a highly complex mixture of about 100–700 different components, where peptides are the major constituents with various biological and pharmacological properties including anticancer activities. In this study, anticancer efficacy of the venom of the Egyptian scorpion Androctonus amoreuxi has been evaluated. In vitro, the human breast cancer MCF-7 cell line was treated with the venom and the IC50 was estimated. In vivo studies, Ehrlich ascites carcinoma (EAC) cells were inoculated into CD-1 mice intraperitoneally to form liquid tumor or subcutaneously to form solid tumor and then treated with intraperitoneal injection with venom (0.22 mg/kg) every other day. The total tumor cells in the ascitic fluid and the size of the solid tumor were assessed after 14 and 30 days, respectively. In addition, the mean survival time (MST), body weight, tumor volume, PCV, viability of tumor cells, CBC, AST, ALP, creatinine, oxidative stress biomarkers (GSH, MDA, PCC), tumor marker Ki67, growth factor VEGF and caspase-3 were measured in normal control, EAC control and venom-treated groups (n = 6). Treatment with venom induced anti-tumor effects against liquid and in solid tumors as indicated by a significant (P < 0.05) reduction in tumor volume/size, count of viable EAC cells, expression of Ki67 and VEGF as well as by remarkable increases in MST and caspase-3 expression as compared to non-treated group. Interestingly, the venom restored the altered hematological and biochemical parameters of tumor-bearing animals and significantly increased their life span. These data indicate to (1) the cytotoxic potential effects of A. amoreuxi on tumor cells via anti-proliferative, apoptotic and anti-angiogenic activities; (2) opening a new avenue for further studies on the anti-cancer effects of this agent.

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“…It can inhibit glioma cell proliferation by regulating their ion channels (Gomes et al 2010). A peptide isolated from this scorpion has proven to be an anti-thrombotic (Petricevich et al 2013) and another polypeptide having dose-dependent inhibitory activity arrested cell cycle of prostate cancer cell line DU-145 at G1 phase (Mishal et al 1 3 2013; Zhang et al 2009). Antiapoptotic role of this polypeptide can be due to highly expressed Bax (proapoptotic) or downregulated Bcl-2 (antiapoptotic) (Zhang et al 2009).…”

Section: As Antineoplastic Agentmentioning

confidence: 99%

“…A peptide isolated from this scorpion has proven to be an anti-thrombotic (Petricevich et al 2013) and another polypeptide having dose-dependent inhibitory activity arrested cell cycle of prostate cancer cell line DU-145 at G1 phase (Mishal et al 1 3 2013; Zhang et al 2009). Antiapoptotic role of this polypeptide can be due to highly expressed Bax (proapoptotic) or downregulated Bcl-2 (antiapoptotic) (Zhang et al 2009). BMHYA1, an enzyme procured by extraction and purification from this scorpion hampers overexpression of CD44 surface marker in cancer cells (Hmed et al 2013).…”

Section: As Antineoplastic Agentmentioning

confidence: 99%

Scorpion Venom–Toxins that Aid in Drug Development: A Review

Ghosh

Roy

Nandi

et al. 2018

Int J Pept Res Ther

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Scorpion venom components have multifaceted orientation against bacterial, viral, fungal infections and other neuronal disorders. They can modulate the ion channels (K + , Na + , Cl − , Ca 2+ ) of our body and this concept has been hypothesized in formulating pharmaceuticals. The triumphant achievement of these venom components as formulated anticancer agent in Phase I and Phase II clinical trials allure researchers to excavate beneficial venom components prohibiting DNA replication in malignant tumor cells. This review brings forth the achievements of Science and Technology in classifying the venom components as therapeutics and further application in drug product development.

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Anti-proliferation Effect of Polypeptide Extracted from Scorpion Venom on Human Prostate Cancer Cells in vitro

Cited by 28 publications

References 37 publications

Scorpion venom component III inhibits cell proliferation by modulating NF-κB activation in human leukemia cells

Scorpion venom component III inhibits cell proliferation by modulating NF-κB activation in human leukemia cells

In vitro and in vivo antitumor effects of the Egyptian scorpion Androctonus amoreuxi venom in an Ehrlich ascites tumor model

Scorpion Venom–Toxins that Aid in Drug Development: A Review

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