Anti-proliferative and Anti-metastatic Potential of High Molecular Weight Secretory Molecules from Probiotic Lactobacillus Reuteri Cell-Free Supernatant Against Human Colon Cancer Stem-Like Cells (HT29-ShE)

Maghsood, Faezeh; Johari, Behrooz; Rohani, Mahdi; Madanchi, Hamid; Saltanatpour, Zohreh; Kadivar, Mehdi

doi:10.1007/s10989-020-10049-z

Cited by 30 publications

(25 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This may be due to the amplifying role of LAS in the expression of MMP-12. In addition to the anti-proliferative, pro-apoptotic, and anti-metastatic effects of lactobacilli [43][44][45], our results showed that L. acidophilus and L. rhamnosus also have remarkable anti-proliferative and anti-metastatic effects on colorectal cancer cells.…”

Section: Discussionmentioning

confidence: 69%

The Inhibition of Proliferation and Invasion of Human Colon Carcinoma Cell Line (Caco-2 cells) by Cell-free Supernatants from Lactobacillus Rhamnosus and Lactobacillus Acidophilus

Shokati

Soleimani

Abroun

2021

Preprint

View full text Add to dashboard Cite

Background: The epidemiological studies indicated that colorectal cancer is one of the most common types of cancer in the world and is considered a leading cause of cancer-related death. The present study aimed to investigate the inhibitory effect of lactobacillus acidophilus supernatant (LAS) and lactobacillus rhamnosus supernatant (LRS) on the growth and invasiveness of the human colon carcinoma cell line (Caco2) in-vitro. Methods: In this experimental study, the anti-proliferative activity and anti-invasion potential of LAS and LRS were determined by MTT and transwell chambers assays, respectively. The expression of mitochondrial membrane potential-9 (MMP-9) and matrix metalloproteinase-12 (MMP12) genes were analyzed by real-time PCR.Results: The results indicated that supernatants of these two lactobacilli had cytotoxic effects on Caco-2 cells at a concentration of 25% v/v and higher. Thus, the minimum concentrations (25% V/V) of supernatants were chosen for further experiments. LAS and LRS could significantly suppress the invasiveness of Caco-2 cells. Also, the expression of MMP12 was significantly increased in Caco-2 cells when treated with LAS, whereas LRS had no significant effect on the invasive capacity and the gene expression levels of MMP12. The expression of MMP-9 was statistically decreased in Caco2 cells treated with LAS and LRS (P<0.00001).Conclusion: In general, it was shown that LAS and LRS exert anti-cancer activity against the growth, invasion, and metastasis of Caco2 cells in-vitro. It seems that these two bacteria could be used as prophylactic and therapeutic agents for the prevention and treatment of colorectal cancer.

show abstract

Section: Discussionmentioning

confidence: 69%

The Inhibition of Proliferation and Invasion of Human Colon Carcinoma Cell Line (Caco-2 cells) by Cell-free Supernatants from Lactobacillus Rhamnosus and Lactobacillus Acidophilus

Shokati

Soleimani

Abroun

2021

Preprint

View full text Add to dashboard Cite

show abstract

“…It was found that L. reuteri containing Lozenges could increase TIMP1 and decrease MMP8 levels, and it was also effective for chronic periodontitis ( _ Ince et al 2015). Moreover it has been demonstrated that high molecular weight compounds derived from L. reuteri PTCC 1655 have an anti-proliferative and anti-metastatic effect on HT29-ShE cells (Maghsood et al 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The primers for desired genes and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (for RT-qPCR were provided from Bioneer (Daejeon, South korea). The sequences of primers used in this study are presented in the previous report (Maghsood et al 2020), in addition to the NOS2 primer (forward 5 0 -GGCCTGTCCTTGGAAATTTC-3 0 and reverse 5 0 -CTGGACTGGAGGTGGCACA-3 0 ).…”

Section: Cells and Reagentsmentioning

confidence: 99%

“…Previously, the anti-cancer characteristics of this probiotic strain have been studied on some cancer cell lines, such as the study by Soltan Dallal et al (2013), in which the efficacy of living L. reuteri PTCC 1655 in preventing the tumour growth and survival of mice from neoplasm malignancy of breast cancer was reported (Soltan Dallal et al 2013). Another study by Maghsood et al (2020) reported the anti-proliferative and anti-metastatic properties of high molecular weight secretory molecules from this strain on the HT29-ShE cell line, which is a cellular model for human colon cancer stem-like cells. Because the probiotic characteristics are strain-dependent (Rajab et al 2020), and there are insufficient reports on the anti-inflammatory characteristics of L. reuteri PTCC 1655, it appeared necessary to design a study to provide information about the anti-inflammatory effects of this probiotic strain of L. reuteri.…”

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

Investigating the anti‐inflammatory effects of high molecular weight secretions from Limosilactobacillus reuteri PTCC 1655 on LPS‐stimulated PMA‐differentiated THP‐1 cells

et al. 2021

View full text Add to dashboard Cite

Aims: This study was done to investigate the anti-inflammatory effects of high molecular weight secretions from Limosilactobacillus reuteri PTCC 1655 probiotic bacteria on lipopolysaccharide (LPS)-stimulated phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 cells. Methods and Results: After culturing the bacterium, the crude cell-free supernatant was fractionated on the basis of molecular weights using ultrafiltration. Also, a heat-killed and sonicated fraction was obtained from the biomass of the bacterial culture. All fractions were used to measure their antiinflammatory effects on PMA-differentiated THP-1 cells following LPS stimulation by quantifying various cellular markers of inflammation. The results demonstrated that various L. reuteri PTCC 1655-derived fractions, especially the >100 kDa supernatant fraction decreased some of the inflammatory cytokines and mediators, including tumour necrosis factor-a, interleukin-1, nitric oxide, cyclooxygenase-2, matrix metalloproteinase-9 and interleukin-6, which are critical for the pathogenesis of some inflammatory diseases. Conclusion: It is concluded that the L. reuteri PTCC 1655-derived high molecular weight fractions significantly reduce inflammation and therefore could be appropriate candidates for future medical studies. Significance and Impact of the Study: Providing new insights about the significance of L. reuteri PTCC 1655-derived extracts and their potential to modulate inflammation.

show abstract

“…The obtained effects against metastasis and apoptosis could be explained via the downregulation of MMP‐9 and COX‐2 in line with the upregulation of TIMP‐1. Therefore, It could be concluded that secretory macromolecules were responsible for the observed effects via modulations of different gene expressions (Maghsood et al., 2020).…”

Section: Safety Aspects Of Postbioticsmentioning

confidence: 99%

Postbiotics produced by lactic acid bacteria: The next frontier in food safety

Moradi

Kousheh

Almasi

et al. 2020

Comp Rev Food Sci Food Safe

195

View full text Add to dashboard Cite

There are many critical challenges in the use of primary and secondary cultures and their biological compounds in food commodities. An alternative is the application of postbiotics from the starter and protective lactic acid bacteria (LAB). The concept of postbiotics is relatively new and there is still not a recognized definition for this term. The word "postbiotics" is currently used to refer to bioactive compounds, which did not fit to the traditional definitions of probiotics, prebiotics, and paraprobiotics. Therefore, the postbiotics may be presently defined as bioactive soluble factors (products or metabolic byproducts), produced by some food-grade microorganisms during the growth and fermentation in complex microbiological culture (in this case named cell-free supernatant), food, or gut, which exert some benefits to the food or the consumer. Many LAB are considered probiotic and their postbiotic compounds present similar or additional health benefits to the consumer; however, this review aimed to address the most recent applications of the postbiotics with food safety purposes. The potential applications of postbiotics in food biopreservation, food packaging, and biofilm control were reviewed. The current uses of postbiotics in the reduction and biodegradation of some food safety-related chemical contaminants (e.g., biogenic amines) were considered. We also discussed the safety aspects, the obstacles, and future perspectives of using postbiotics in the food industry. This work will open up new insights for food applications of postbiotics prepared from LAB.

show abstract

Anti-proliferative and Anti-metastatic Potential of High Molecular Weight Secretory Molecules from Probiotic Lactobacillus Reuteri Cell-Free Supernatant Against Human Colon Cancer Stem-Like Cells (HT29-ShE)

Cited by 30 publications

References 55 publications

The Inhibition of Proliferation and Invasion of Human Colon Carcinoma Cell Line (Caco-2 cells) by Cell-free Supernatants from Lactobacillus Rhamnosus and Lactobacillus Acidophilus

The Inhibition of Proliferation and Invasion of Human Colon Carcinoma Cell Line (Caco-2 cells) by Cell-free Supernatants from Lactobacillus Rhamnosus and Lactobacillus Acidophilus

Investigating the anti‐inflammatory effects of high molecular weight secretions from Limosilactobacillus reuteri PTCC 1655 on LPS‐stimulated PMA‐differentiated THP‐1 cells

Postbiotics produced by lactic acid bacteria: The next frontier in food safety

Contact Info

Product

Resources

About