Anti-proliferative effect of 20-hydroxyecdysone in a lepidopteran cell line

20-hydroxyecdysone-induced bone morphogenetic protein-2-dependent osteogenic differentiation through the ERK pathway in human periodontal ligament stem cells

“…In previous studies, effects of 20-Hydroxyecdysone on proliferation appeared complex. It stimulated proliferation in some cell types but inhibited in others (Luedeman and Levine, 1996;Fujiwara and Ogai, 2001;Auzoux-Bordenave et al, 2002;Tang et al, 2008). In our study, 20-Hydroxyecdysone promoted PDLS cell proliferation.…”

The 20-hydroxyecdysone-induced cellular arrest in G2 phase is preceded by an inhibition of cyclin expression

“…20-Hydroxyecdysone was a gift of Dr. René Lafont and JH III was purchased from Sigma (France). The 10 À6 M concentration of 20E that was used was close to the physiological concentration of ecdysteroids in Lepidoptera (Lynn and Oberlander, 1983;Auzoux-Bordenave et al, 2002;Debernard et al, 2001). Stock solutions were prepared in ethanol and stored at À20 v C in glass vials coated with 1% polyethylene glycol 20,000 to decrease the possible adsorptive loss.…”

“…An exposure of IAL-PID2 cells to 20E at 10 À6 M for 24 h leads to the inhibition of cell proliferation (Auzoux-Bordenave et al, 2002). To determine whether this effect of 20E could induce a change in cell distribution within cycle phases, we analysed the IAL-PID2 cells by fluorescent flow cytometry.…”

“…Embryonic cells from Drosophila melanogaster (Cherbas et al, 1980;Couderc and Dastugue, 1990;Dinan et al, 1990;Sobrier et al, 1986), and epithelial cells from Manduca sexta (Lynn and Oberlander, 1981), Chironomus tentans (Spindler- Barth et al, 1992) as well as IAL-PID2 cells derived from imaginal wing discs of Plodia interpunctella (Lynn and Oberlander, 1983;Cassier et al, 1991;Porcheron et al, 1991;Hatt et al, 1994;Auzoux-Bordenave et al, 2002) respond to 20E exposure by an arrest of cell growth and long term morphological differentiation. Fluorescent flow cytometry analysis of Drosophila Kc cell cycle has revealed that 20E-induced inhibition growth resulted from an arrest of cells in the G2 phase (Stevens et al, 1980).…”

Comparative effects of a non-steroidal ecdysone agonist RH-5992 and 20-hydroxyecdysone in a lepidopteran cell line (IAL-PID2)

“…To compare the action of RH-5992 with that of 20E in the control of cell cycle, we followed within cycle phases, the distribution of treated cells either by RH-5992 or 20E at 4 Â 10 À8 M for 36 h. This period of time corresponds to the population doubling time in our model (Hatt et al, 1997;Auzoux-Bordenave et al, 2002). The flow cytometry analysis showed that the untreated cells were 10 -8 10 -7 10 -6 Concentration (M) Fig.…”