2023
DOI: 10.1038/s41598-023-31443-9
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Anti-proliferative effect of melatonin in human hepatoma HepG2 cells occurs mainly through cell cycle arrest and inflammation inhibition

Abstract: Hepatocellular carcinoma (HCC) is the major lethal primary liver malignant worldwide. Although, melatonin has various antitumor bioactivities; there is a requirement for more investigations to elucidate the not discussed effects, and the controversial responses of the treatment with melatonin on targets mediated in HCC. To achieve the aim of the present study, HCC-HepG2 cells were treated with different concentrations of melatonin at various time intervals. The selected minimal proliferation inhibition doses o… Show more

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Cited by 7 publications
(2 citation statements)
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References 43 publications
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“…The RT and subsequent PCR cycling condition was as follow: 50 °C for 10 min, 95 °C for 5 min, 60 °C for 30 s, and then 95 °C for 15 s; the number of cycles was 40 cycles. Bio-Rad Miniopticon™ real-time PCR cycler was used for quantitative estimation [ 24 26 ].…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The RT and subsequent PCR cycling condition was as follow: 50 °C for 10 min, 95 °C for 5 min, 60 °C for 30 s, and then 95 °C for 15 s; the number of cycles was 40 cycles. Bio-Rad Miniopticon™ real-time PCR cycler was used for quantitative estimation [ 24 26 ].…”
Section: Methodsmentioning
confidence: 99%
“…Doxorubicin-resistant MDA-MB-231 cells were grown at a density of 1 × 10 6 cells/ well onto a six-well plate and then transfected with miscript miRNA negative control or miR-34a-5p mimics for 48 h. Cells were then washed with Dulbecco’s phosphate-buffered saline (DPBS), trypsinized using 0.05% trypsin–EDTA, fixed in 70% ethanol in DPBS, and stored at 4 °C overnight. Cells were incubated with 50 µg/ ml PI (Thermo Scientific) containing 8 µg/ml RNase A in the dark at 37 °C for 30 min [ 24 , 26 , 30 ]. Cells were then analyzed by NovoCyte flowcytometer (Agilent, USA).…”
Section: Methodsmentioning
confidence: 99%