Anti-proliferative,in vitroantioxidant, and cellular antioxidant activities of the leaf extracts fromPolygonum minusHuds: Effects of solvent polarity

Abstract

The present study reports the antioxidant and anti-proliferative activities of Polygonum minus leaf extracts obtained through sequential extraction using four solvents of varying polarities (i.e. hexane (HX), ethyl acetate (EA), methanol, and water). The antioxidant potential was evaluated by measuring the total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), 2,2′-azinobis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical-scavenging, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical-scavenging, superoxide anion and nitric oxide scavenging, ferrous ion-chelating (FIC), and cellular antioxidant activity (CAA) assays. The highest antioxidant potential was generally shown by the methanol extract (PM-MeOH). PM-MeOH exhibited the highest values for TPC (174.00 ± 0.18 mg GAE/g), TFC (53.19 ± 0.71 mg GAE/g), FRAP (1728.33 ± 0.96 µmol Fe 2+ /g), ABTS (226.25 ± 4.25 µmol TE/g), DPPH (1276.81 ± 7.08 µmol TE/g), and nitric oxide scavenging assays (IC 50 , 675 ± 32.33 µg/mL). In the CAA assay, PM-MeOH dose-dependently inhibited the peroxyl radical-induced oxidation of 2ʹ,7ʹ-dichlorodihydrofluorescein (DCFH 2) to 2ʹ,7ʹ-dichlorofluorescein (DCF) in HCT116 cells, with an EC 50 value of 263.92 ± 21.60 µg/mL. Liquid chromatography and mass spectrometry analyses of PM-MeOH suggested the presence of tannins and flavonoids including apigetrin, hyperoside, isoquercetin, astragalin, miquelianin, quercetin, and quercitrin. P. minus hexane (PM-HX) and ethyl acetate (PM-EA) extracts showed selective cytotoxicity towards HCT116 with IC 50 values of 40.00 ± 0.83 µg/mL and 43.18 ± 0.67 µg/mL, respectively. Taken together, these results highlight the potential of P. minus as a source of bioactive phytochemicals that may be useful in cancer therapeutics and nutraceutical industry.

NMR-Based Metabolomics Profiling for Radical Scavenging and Anti-Aging Properties of Selected Herbs

“…Secondary metabolites of the aromatic region (δ 5.50–9.00) were identified as quercetin, quercetin-3- O -rhamnoside [47,80], quercetin-3- O -glucoside [80], quercetin-3- O -glucuronide [81], quercetin-3- O -arabinofuranoside [47,80], rutin, myricetin derivatives, catechin, epicatechin, isorhamnetin, astragalin, apigenin, chlorogenic acid, gallic acid, coumaric acid, fumaric acid, formic acid, 3-methylxanthine and serotonin. Various studies have shown the presence of phenolic compounds and secondary metabolites, in particular flavonoids in P. minus leaves [82,83,84], as found also in this study.…”

Phenolic profile and biological activities ofMicromeria graeca(L.) Benth. ex Rchb.

“…These compounds exhibit a wide range of pharmacological effects that are linked to their antioxidant properties. [10,11] Phenolic compounds have been reported to be excellent free radical scavengers due to their strong hydrogen-donating ability. [10,12] Reviewing the literature, nothing was reported concerning the phenolics of M. graeca (L.) Benth.…”

Optimization of total flavonoid content of ethanolic extract of Persicaria pulchra (Bl.) Soják for the inhibition of α-glucosidase enzyme

“…TPC and TFC of the plant are 388 mg GA/g and 423 mg QUE/g, respectively. Interestingly, TPC and TFC of P. pulchra were much higher (35) 0 (70) 0 (10) 0 (65) 437 ± 11 2 (75) 0 (70) 0 (10) 0 (65) 440 ± 9 0 (55) −2 (10) 0 (10) 0 (65) 449 ± 4 0 (55) 2 (130) 0 (10) 0 (65) 426 ± 14 0 (55) 0 (70) −2 (6) 0 (65) 504 ± 10 0 (55) 0 (70) 2 (14) 0 (65) 474 ± 14 0 (55) 0 (70) 0 (10) −2 (35) 461 ± 12 0 (55) 0 (70) 0 (10) 2 (95) 456 ± 13 0 (55) 0 (70) 0 (10) 0 (65) 534 ± 10 0 (55) 0 (70) 0 (10) 0 (65) 529 ± 9 0 (55) 0 (70) 0 (10) 0 (65) 536 ± 12 0 (55) 0 (70) 0 (10) 0 (65) 521 ± 14 0 (55) 0 (70) 0 (10) 0 (65) 529 ± 7 0 (55) 0 (70) 0 (10) 0 (65) 521 ± 12 0 (55) 0 (70) 0 (10) 0 (65) 528 ± 8 0 (55) 0 (70) 0 (10) 0 (65) 530 ± 7 0 (55) 0 (70) 0 (10) 0 (65) 532 ± 15 0 (55) 0 (70) 0 (10) 0 (65) 536 ± 7 0 (55) 0 (70) 0 (10) 0 (65) 522 ± 10 0 (55) 0 (70) 0 (10) 0 (65) 529 ± 9 than those of other plants in the Persicaria family, such as P. odoratum and P. minus [28,29]. The crude ethanolic extract of P. pulchra exhibited good inhibition of α-glucosidase with IC 50 of 33.53 ± 1.25 µg/mL, which is comparable to those of some anti-diabetic Vietnamese plants, like N. mirabilis, P. urinaria, and K. candel [30].…”