Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G0/G1 arrest and apoptosis in Jurkat leukaemia cells

Pereira, Andreia S.P.; Bester, Megan Jean; Soundy, Puffy; Apostolides, Zeno

doi:10.3109/13880209.2015.1129545

Cited by 15 publications

(11 citation statements)

References 75 publications

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“…PEL+LAT were able to statistically reduce S. aureus and E. coli growth, effect that was longer than that observed for penicillin/streptomycin. The administration of the single compounds still reduced bacteria growth; however, this effect was observed at 6 h rather than at 5 h. Moreover, this effect was not due to antioxidant activity of the single compounds as already reported [ 4 , 20 ]. Indeed, the sole compounds were not able to exert antioxidant activities, rather, they showed bacteriostatic activity although at later time points.…”

Section: Discussionsupporting

confidence: 72%

“…Inspired by the healing of his tuberculosis, Charles Henry Stevens introduced this phytomedical drug to England in 1897 [ 2 ], and since then, the use of this plant has increased more and more interest because of its beneficial properties. Pharmacological studies have demonstrated the usefulness of PEL in the treatment of several disorders due to its anti-inflammatory, antioxidant and immunomodulatory activities [ 3 ] and its antiproliferative effect [ 4 ]. In our very recent paper, we demonstrated that PEL together with other natural products can exert antimicrobial activities [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant and antimicrobial properties of Pelargonium sidoides DC and lactoferrin combination

Terlizzi

Colarusso

Maio³

et al. 2020

Bioscience Reports

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Lactoferrin (LAT), a multi-functional protein involved in numerous physiological functions, and the medicinal plant Pelargonium sidoides DC (PEL), have been described for their anti-inflammatory properties. Because the main advantage of natural products consists in administering them in combination rather than as single compound, we aimed to understand whether the combination of PEL and LAT, herein PELIRGOSTIM, could still prove beneficial or additive/synergistic activities during inflammatory conditions. To pursue this goal, we used macrophagic cells (J774.1) and treated them with PEL and LAT in a concentration-dependent manner. We found that PELIRGOSTIM was able to reduce the levels of reactive oxygen species (ROS) and nitrite, effects that were correlated to the release of lower levels of IL-1β after LPS treatment. In addition, the combination of PEL and LAT showed bacteriostatic activities against S. aureus and E. coli which had limited growth starting from 5 hours up to 20 hours. This effect was stronger than that observed for penicillin/streptomycin. Our results provide PELIRGOSTIM as an innovative combination of natural products capable to prevent inflammation-, oxidative stress- and microbial-related disorders.

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Section: Discussionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Antioxidant and antimicrobial properties of Pelargonium sidoides DC and lactoferrin combination

Terlizzi

Colarusso

Maio³

et al. 2020

Bioscience Reports

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“…In previous studies, we found anti-proliferative activity for a commercial radix mother tincture of P. sidoides in vitro and through a bioactivity-guided approach; we isolated seven fractions with significant anti-proliferative activity [17,18]. Liquid chromatography with tandem mass spectrometry detection (LC-MS/MS) analysis of these fractions revealed the presence of phenolic compounds gallic acid, koaburaside, proanthocyanidin dimers as well as a trimer, dihydroxycoumarin sulfates, trihydroxycoumarin as well as three turgorins namely leaf movement factor (LMF) 2, LMF3 and the periodic leaf movement factor 1 (PLMF1).…”

Section: Introductionmentioning

confidence: 93%

Exploring the anti-proliferative activity of Pelargonium sidoides DC with in silico target identification and network pharmacology

2017

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Pelargonium sidoides DC (Geraniaceae) is a medicinal plant indigenous to Southern Africa that has been widely evaluated for its use in the treatment of upper respiratory tract infections. In recent studies, the anti-proliferative potential of P. sidoides was shown, and several phenolic compounds were identified as the bioactive compounds. Little, however, is known regarding their anti-proliferative protein targets. In this study, the anti-proliferative mechanisms of P. sidoides through in silico target identification and network pharmacology methodologies were evaluated. The protein targets of the 12 phenolic compounds were identified using the target identification server PharmMapper and the server for predicting Drug Repositioning and Adverse Reactions via the Chemical-Protein Interactome (DRAR-CPI). Protein-protein and protein-pathway interaction networks were subsequently constructed with Cytoscape 3.4.0 to evaluate potential mechanisms of action. A total of 142 potential human target proteins were identified with the in silico target identification servers, and 90 of these were found to be related to cancer. The protein interaction network was constructed from 86 proteins involved in 209 interactions with each other, and two protein clusters were observed. A pathway enrichment analysis identified over 80 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enriched with the protein targets and included several pathways specifically related to cancer as well as various signaling pathways that have been found to be dysregulated in cancer. These results indicate that the anti-proliferative activity of P. sidoides may be multifactorial and arises from the collective regulation of several interconnected cell signaling pathways.

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“…Geranium essential oils are known to exhibit anti-cancer activity [21,22]. Previous studies have shown that the Pelargonium species have cytotoxic activity on cancer cells [2,7,23].…”

Section: Iscussionmentioning

confidence: 99%

Total Phenolic Content, Antioxidant and Cyto-/Genotoxic Activities of Pelargonium Quercetorum Agnew in Human Breast Cancer Cells

Arı¹,

Çelikler²,

Karakaş³

et al. 2017

J Clin Exp Invest

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Because the current cancer treatment success rate is not sufficient, plants have been gaining importance as a possible source for anti-cancer compounds. This study aimed to investigate both the genotoxic and cytotoxic activities of methanol extracts of Pelargonium quercetorum Agnew (P. quercetorum), which is traditionally used for its antihelminthic activity, but has not yet been studied for its effect in breast cancer cells. Methods: In this study, the cyto-genotoxic activities of methanol extracts of Pelargonium quercetorum were investigated in human breast cancer cells (MCF-7 and MDA-MB-231). The cytotoxic effect of the extract on these cells was evaluated by MTT and ATP viability assays. The mode of cell death (apoptosis/necrosis) was determined using fluorescence microscopy and biochemical methods. Genotoxic activity was studied with Comet assay. In addition, the total phenolic content and antioxidant capacity were determined by the Folin-Ciocalteu and ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] methods, respectively. Results: Results of this study reveal that Pelargonium quercetorum has a large total phenolic content and high antioxidant capacity. Pelargonium quercetorum induced anti-growth effects in a dose-dependent manner in cancer cells. The extract killed the cells by apoptosis as evidenced by the presence of pyknotic nucleus and annexin V-FITC positivity. The extract also exerted genotoxic activity at relatively low doses. Conclusion: These results suggest that Pelargonium quercetorum induces apoptosis-like cell death by causing DNA damage in breast cancer cells.

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Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Cited by 15 publications

References 75 publications

Antioxidant and antimicrobial properties of Pelargonium sidoides DC and lactoferrin combination

Antioxidant and antimicrobial properties of Pelargonium sidoides DC and lactoferrin combination

Exploring the anti-proliferative activity of Pelargonium sidoides DC with in silico target identification and network pharmacology

Total Phenolic Content, Antioxidant and Cyto-/Genotoxic Activities of Pelargonium Quercetorum Agnew in Human Breast Cancer Cells

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