Anti-proteolytic capacity and bonding durability of proanthocyanidin-biomodified demineralized dentin matrix

Abstract

Our previous studies showed that biomodification of demineralized dentin collagen with proanthocyanidin (PA) for a clinically practical duration improves the mechanical properties of the dentin matrix and the immediate resin–dentin bond strength. The present study sought to evaluate the ability of PA biomodification to reduce collagenase-induced biodegradation of demineralized dentin matrix and dentin/adhesive interfaces in a clinically relevant manner. The effects of collagenolytic and gelatinolytic activity on PA-biomodified demineralized dentin matrix were analysed by hydroxyproline assay and gelatin zymography. Then, resin-/dentin-bonded specimens were prepared and challenged with bacterial collagenases. Dentin treated with 2% chlorhexidine and untreated dentin were used as a positive and negative control, respectively. Collagen biodegradation, the microtensile bond strengths of bonded specimens and the micromorphologies of the fractured interfaces were assessed. The results revealed that both collagenolytic and gelatinolytic activity on demineralized dentin were notably inhibited in the PA-biomodified groups, irrespective of PA concentration and biomodification duration. When challenged with exogenous collagenases, PA-biomodified bonded specimens exhibited significantly less biodegradation and maintained higher bond strengths than the untreated control. These results suggest that PA biomodification was effective at inhibiting proteolytic activity on demineralized dentin matrix and at stabilizing the adhesive/dentin interface against enzymatic degradation, is a new concept that has the potential to improve bonding durability.

Dentin Sealing and Bond Strength Evaluation of Hema-Free and Multi-Mode Adhesives to Biomodified Dentin

“…Proanthocyanidin from grape seed extract is a natural safe compound and works as well as glutaraldehyde (27). Proanthocyanidin extract stabilizes and increases the density of type-I collagen network (26), which improves the dentin mechanical properties and decreases the swelling ratio that may indicate a matrix resistance against enzymatic degradation (11,26). The results of the present study did not show a statistically difference between glutaraldehyde and proanthocyanidin extract at 24 h for all adhesives studied.…”

“…Glutaraldehyde is a synthetic aldehyde collagen cross-linking agent that decreases the rate of collagen degradation (26). However, it presents high cytotoxicity (8), which limited their clinical use.…”

Effect of proanthocyanidin on push-out bond strength between fiber post and root dentin- An in-vitro study

“…19 For MMPs, cross-linkage of the catalytic sites of these enzymes and inhibition of other non-collagenous proteins that activate these enzymes also have been hypothesized. 20,21 Possibly, the combined cross-linking and anti-collagenolytic effects of PAs are responsible for preventing degradation of dentin collagen within the hybrid layer. 8 Results of the failure analysis by chi-square test, showed that a highly significant difference existed in between Groups 1 & 2 (p <0.0001).…”

Demineralized Dentin as a Semi‐Rigid Barrier for Guiding Periodontal Tissue Regeneration

“…Advantages of acid etching include high maneuverability, low cost, and non‐toxity 27 . However, studies on acid‐processed dentin have focused generally on dental acid corrosion materials rather than altering the characteristics of dentin for application as possible bone substitute material for clinical application 28–30 . Thus, parameters of acid etching and concrete physiochemical properties of treated dentin tissue remain to be explored.…”