Anti-quorum sensing activity of medicinal plants in southern Florida

Adonizio, Allison L.; Downum, Kelsey R.; Bennett, Bradley C.; Mathee, Kalai

doi:10.1016/j.jep.2005.11.025

Cited by 250 publications

(184 citation statements)

References 43 publications

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“…Anti-QS activity has also been noted in a number of traditional medicinal plants (Adonizio et al, 2006). Rhubarb (named Dahuang in Chinese), a medicinal plant, displays diverse pharmacological activities such as bacteriostatic, antiviral, antifungal and antitumour activities (Huang & Zhen, 2001;Kim et al, 2004;Basu et al, 2005;Huang et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Screening for novel quorum-sensing inhibitors to interfere with the formation of Pseudomonas aeruginosa biofilm

Ding

Yin

et al. 2011

Journal of Medical Microbiology

126

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The objective of this study was to screen for novel quorum-sensing inhibitors (QSIs) from traditional Chinese medicines (TCMs) that inhibit bacterial biofilm formation. Six of 46 active components found in TCMs were identified as putative QSIs based on molecular docking studies. Of these, three compounds inhibited biofilm formation by Pseudomonas aeruginosa and Stenotrophomonas maltophilia at a concentration of 200 mM. A fourth compound (emodin) significantly inhibited biofilm formation at 20 mM and induced proteolysis of the quorum-sensing signal receptor TraR in Escherichia coli at a concentration of 3-30 mM. Emodin also increased the activity of ampicillin against P. aeruginosa. Therefore, emodin might be suitable for development into an antivirulence and antibacterial agent. INTRODUCTIONPseudomonas aeruginosa, an opportunistic human pathogen, may cause acute infections in hospitalized patients, can be isolated from the environment, particularly from soil and water, and it regularly contaminates medical devices (Stover et al., 2000). It is also the predominant cause of chronic lung infection in cystic fibrosis patients (Frederiksen et al., 1997) and has recently been recognized as one of the main causes of chronic wound infections (Gjødsbøl et al., 2006). P. aeruginosa can infect patients by producing a wide range of virulence factors, the expression levels of which are tightly regulated. Key to this regulation is cell density-dependent cell-to-cell signalling, which is termed quorum sensing (QS) (Rumbaugh et al., 2000). As in many other bacteria, QS controls secretion of virulence factors (Mittal et al., 2006), biofilm formation (Waters et al., 2008) and the exchange of DNA (Fuqua & Winans, 1996) in P. aeruginosa. The biofilm mode of growth is recognized as an important bacterial trait that is relevant to infections (Costerton et al., 1994). Many infections involve the formation of bacterial biofilms, which are bacterial communities that settle and proliferate on surfaces and are covered by exopolymers (Lewis, 2007). Once established, biofilms are difficult to eradicate and become a source of secondary infection (Jones et al., 2009). Moreover, bacteria embedded in biofilms are more tolerant than planktonic cells of antibiotics (Donlan & Costerton, 2002;Drenkard, 2003). The dose of antibiotics needed in this situation will often exceed the highest deliverable dose, which makes efficient treatment impossible.QS, as a regulatory mechanism, enables bacteria to make collective decisions with respect to the expression of a specific set of genes that involve the production, release and subsequent detection of chemical signalling molecules, such as N-acylhomoserine lactones (AHLs) that are commonly used by Gram-negative bacteria. When the concentration of AHLs reaches a certain threshold level, binding to a receptor molecule (for example, LuxR) is promoted and the activated LuxR-AHL complex forms dimers or polymers, which, in turn, act as transcriptional regulators of target genes in the QS regulon (Parsek & Greenbe...

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Section: Discussionmentioning

confidence: 99%

Screening for novel quorum-sensing inhibitors to interfere with the formation of Pseudomonas aeruginosa biofilm

Ding

Yin

et al. 2011

Journal of Medical Microbiology

126

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“…Effects of downregulated virulence factors in vitro were translated to decreases in pathogenicity of P. aeruginosa on plant tissues (using Arabidopsis thaliana as a model) and in an invertebrate animal model (C. elegans infection); however, a molecular explanation for how curcumin affects P. aeruginosa quorum sensing remains to be seen. Separately, in studies of 50 medicinal plants from South Florida, six plants representing five different families were found to have QSI activity against C. violaceum and A. tumefaciens and could inhibit production of elastase, pyoverdin, and biofilms of P. aeruginosa (277,278). Several flavenoid-like compounds were found to be produced by one of these species, a member of the Combretum family, and the molecule catachin (a flavan-3-ol) was one compound isolated that accounted for these activities (136).…”

Section: Natural-product Qs Inhibitorsmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

690

556

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SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

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“…Deeper understanding on QS has led to its role in controlling the production of virulence factors such as exopolysaccharide synthesis, biofilm formation, swarming motility, pigment production and antibiotic production in some pathogenic bacteria [3,4] . Owing to the importance of QS during bacterial pathogenesis, interfering with this mechanism is being considered as a rational strategy to attenuate their virulence [5] . Increasing emergence of antibiotic resistance in Gram negative bacteria also demands alternative strategies to combat bacterial infections and anti-QS approach is being viewed as an attractive alternative.…”

Section: Introductionmentioning

confidence: 99%