Anti-Quorum Sensing Activity of the Traditional Chinese Herb, Phyllanthus amarus

Priya, Kumutha; Yin, Wai‐Fong; Chan, Kok‐Gan

doi:10.3390/s131114558

Cited by 34 publications

(19 citation statements)

References 39 publications

(56 reference statements)

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“…Jamming of QS by IM from minced beef, salami, soft cheese, fresh salad, and chicken pâté (for example, LAB, Brochothrix thermosphacta , Pseudomonas spp., Enterobacteriaceae, and enterococci) could reduce the L. monocytogenes biofilms formation (Al‐Zeyara and others ). Anti‐QS strategies have been studied and reported by Priya and others (). Phytochemicals are also reported to possess anti‐QS and antibiofilm activities in different microorganisms such as Y. enterocolitica and Erwinia carotovora (Truchado and others ), Chromobacterium violaceum (Borges and others ), and E. coli O157:H7 (Lee and others ).…”

Section: Novel Concepts For the Control Of Mixed‐species Biofilmsmentioning

confidence: 99%

The Paradox of Mixed‐Species Biofilms in the Context of Food Safety

Jahid

2014

Comp Rev Food Sci Food Safe

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Formation of mixed-species biofilms constitutes a common adaptation of foodborne pathogens and indigenous microbiota for prolonged survival in their food niche. Nevertheless, the potential role of mixed-species biofilms in food safety remains to be elucidated. The formation of mixed-species biofilms on food and food processing surfaces depends on various physical, chemical, and biological processes including species composition, especially of the indigenous microbiota and nutrients, food types, temperature, quorum sensing, extracellular polymeric substance (EPS) production, biofilms maturation, and dispersal steps. Compared to monospecies, mixed-species are highly resistant to antimicrobials, possibly due to higher EPS production, internalization into food, fitness of species, denser and thicker biofilms maturation, and interspecific protection of 1 species by others, although there are much debate among studies. The fitness of mixed-species biofilms populations is suggested to be of a cooperative, competitive, or neutral nature based on the genetic background of the involved species. Currently, various methods using microarray, confocal microscopy, proteomics, and selective media are being explored for the detection of mixed-species biofilms to resolve the conflict issues. Here, we review recent progress in this emerging field in the context of food safety and propose that novel and alternative techniques like antiquorum sensing, antibiofilms, enzymes, hurdle techniques, and bacteriophages will significantly help to control the formation of mixed-species biofilms for enhanced food safety. The next challenge will be to integrate the fitness and resistance patterns of mixed-species biofilms in the laboratory with those of natural settings.

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Section: Novel Concepts For the Control Of Mixed‐species Biofilmsmentioning

confidence: 99%

The Paradox of Mixed‐Species Biofilms in the Context of Food Safety

Jahid

2014

Comp Rev Food Sci Food Safe

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“…Crude extracts of many plant parts were shown to possess anti-QS activity using C. violaceum as a model bacterium [19], [20], [21]. Crude plant extracts are often found to be more effective than isolated constituents at an equivalent dose perhaps owing to positive interactions between components of whole plant extracts.…”

Section: Introductionmentioning

confidence: 99%

Guava Leaf Extract Inhibits Quorum-Sensing and Chromobacterium violaceum Induced Lysis of Human Hepatoma Cells: Whole Transcriptome Analysis Reveals Differential Gene Expression

et al. 2014

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Quorum sensing (QS) is a process mediated via small molecules termed autoinducers (AI) that allow bacteria to respond and adjust according to the cell population density by altering the expression of multitudinous genes. Since QS governs numerous bioprocesses in bacteria, including virulence, its inhibition promises to be an ideal target for the development of novel therapeutics. We found that the aqueous leaf extract of Psidium guajava (GLE) exhibited anti-QS properties as evidenced by inhibition of violacein production in Chromobacterium violaceum and swarming motility of Pseudomonas aeruginosa. The gram-negative bacterium, C. violaceum is a rare pathogen with high mortality rate. In this study, perhaps for the first time, we identified the target genes of GLE in C. violaceum MTCC 2656 by whole transcriptome analysis on Ion Torrent. Our data revealed that GLE significantly down-regulated 816 genes at least three fold, with p value≤0.01, which comprises 19% of the C. violaceum MTCC 2656 genome. These genes were distributed throughout the genome and were associated with virulence, motility and other cellular processes, many of which have been described as quorum regulated in C. violaceum and other gram negative bacteria. Interestingly, GLE did not affect the growth of the bacteria. However, consistent with the gene expression pattern, GLE treated C. violaceum cells were restrained from causing lysis of human hepatoma cell line, HepG2, indicating a positive relationship between the QS-regulated genes and pathogenicity. Overall, our study proposes GLE as a QS inhibitor (QSI) with the ability to attenuate virulence without affecting growth. To the best of our knowledge, this is the first report which provides with a plausible set of candidate genes regulated by the QS system in the neglected pathogen C. violaceum.

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“…25 There are several reports in literature that propose different mechanisms of quorum sensing inhibition by natural products, either by inhibition of the signal molecule biosynthesis, 26 preventing the binding of the AHL molecules to its receptors 6 or enzymatic inactivation and biodegradation of the quorum sensing signalling molecules. 5 Study from Zahin et al 13 Chong et al 28 and Priya et al 29 also revealed quorum sensing property of Mangifera indica, Punica granatum, Myristica cinnamomea and Phyllanthus amarus, which showed efficacy in regulating violacein production of C. violaceum and inhibiting motility of P. aeruginosa. The result in our study have revealed that the ethanol extract of T. catappa leaves and A. alitilis flower significantly inhibit the quorum sensing mechanism of P. aeruginosa PAO1 as indicated by a reduction in pyocyanin production, inhibition of P. aeruginosa motility, reduction in LasA protease production and inhibition of P. aeruginosa biofilm formation which is an important trait for its pathogenicity.…”

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confidence: 98%

Attenuation of Pseudomonas aeruginosa Virulence by Some Indonesian Medicinal Plants Ethanolic Extract

Pratiwi¹,

Handayani²

2018

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Context:One strategy to address the emergence of infectious diseases supported by the increasing cases of microbial antibiotic resistance is the development of anti-pathogenic drugs, a quorum quenching compound(s) capable of inhibiting microbial communication (quorum sensing inhibitor). Pseudomonas aeruginosa is one of the most common source of chronic opportunistic infections, which demonstrates the most consistent resistance to antimicrobial agents. Quorum sensing (QS) controls secretion of virulence factors in P. aeruginosa. Aim: This study aims to discover quorum sensing inhibitors (QSI) from some Indonesian medicinal plants ethanol extract to analyze their inhibitory activities against QS-mediated virulence factors in P. aeruginosa. Settings and Design: In-vitro experimental study-laboratory setting Materials and Methods: Indonesian medicinal plant ethanolic extracts were tested for their capability to inhibit P. aeruginosa motility, biofilm formation using microtiter plate method, pyocyanin and LasA production using LasA staphylolytic assay. Statistical analysis used: Statistical significance of the data were determined using one way ANOVA, followed by Dunnett's test. Differences were considered significant with P values of 0.05 or less. Results: Ethanolic extract of T. catappa leaves and A. alitilis flower capable to inhibit P. aeruginosa motility as well as pyocyanin production and biofilm formation. Both extracts also showed capability in reducing LasA protease production. Conclusion: T. catappa and A. alitilis are an interesting sources of innovative plant derived quorum quenching compound(s), thus can be used in the development of new antipathogenic drug.

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Anti-Quorum Sensing Activity of the Traditional Chinese Herb, Phyllanthus amarus

Cited by 34 publications

References 39 publications

The Paradox of Mixed‐Species Biofilms in the Context of Food Safety

The Paradox of Mixed‐Species Biofilms in the Context of Food Safety

Guava Leaf Extract Inhibits Quorum-Sensing and Chromobacterium violaceum Induced Lysis of Human Hepatoma Cells: Whole Transcriptome Analysis Reveals Differential Gene Expression

Attenuation of Pseudomonas aeruginosa Virulence by Some Indonesian Medicinal Plants Ethanolic Extract

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