Anti-quorum sensing, antibiofilm, and antibacterial activities of extracts of <i>Centella asiatica</i> L. leaves, and <i>in vitro</i> derived leaves-calli through tissue culture: a potential for biofouling-prevention

Khan, Mo Ahamad; Khan, Haris M.; Ganie, Irfan Bashir; Kumar, Sachin; Shahzad, Anwar; Çeli̇k, İsmail; Shahid, Mohammad

doi:10.1080/08927014.2022.2117034

Cited by 18 publications

(16 citation statements)

References 42 publications

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“…Sub-MICs are concentrations below the MIC. was used as a control (Khan et al, 2022). Incubated plates were observed for inhibition of purple colored violacein.…”

Section: Evaluation Of the Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

Attenuation of quorum sensing regulated virulence functions and biofilm of pathogenic bacteria by medicinal plant Artemisia annua and its phytoconstituent 1, 8‐cineole

Khan,

Shahid,

Celik

et al. 2023

Microscopy Res & Technique

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The emergence of multidrug resistance (MDR) in bacterial pathogens is a serious public health concern. A significant therapeutic target for MDR infections is the quorum sensing‐regulated bacterial pathogenicity. Determining the anti‐quorum sensing abilities of certain medicinal plants against bacterial pathogens as well as the in‐silico interactions of particular bioactive phytocompounds with QS and biofilm‐associated proteins were the objectives of the present study. In this study, 6 medicinal plants were selected based on their ethnopharmacological usage, screened for Anti‐QS activity and Artemisia annua leaf extract (AALE) demonstrated pigment inhibitory activity against Chromobacterium violaceum CV12472. Further, the methanol active fraction significantly inhibited the virulence factors (pyocyanin, pyoverdine, rhamnolipid and swarming motility) of Pseudomonas aeruginosa PAO1 and Serratia marcescens MTCC 97 at respective sub‐MICs. The inhibition of biofilm was determined using a microtiter plate test and scanning electron microscopy. Biofilm formation was impaired by 70%, 72% and 74% in P. aeruginosa, C. violaceum and S. marcescens, respectively at 0.5xMIC of the extract. The phytochemical content of the extract was studied using GC–MS and 1, 8‐cineole was identified as major bioactive compound. Furthermore, 1, 8‐cineole was docked with quorum sensing (QS) proteins (LasI, LasR, CviR, and rhlR) and biofilm proteins (PilY1 and PilT). In silico docking and dynamics simulations studies suggested interactions with QS‐receptors CviR’, LasI, LasR, and biofilm proteins PilY1, PilT for anti‐QS activity. Further, 1, 8‐cineole demonstrated 66% and 51% reduction in violacein production and biofilm formation, respectively to validate the findings of computational analysis. Findings of the present investigation suggests that 1, 8‐cineole plays a crucial role in the QS and biofilm inhibitory activity demonstrated by Artemisia annua extract.Research Highlights Artemisia annua leaf extract (AALE) methanol fraction demonstrated broad‐spectrum QS and biofilm inhibition Scanning electron microscopy (SEM) confirmed biofilm inhibition Molecular docking and simulation studies suggested positive interactions of 1,8‐cineol with QS‐receptors and biofilm proteins

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“…Sub-MICs are concentrations below the MIC. was used as a control (Khan et al, 2022). Incubated plates were observed for inhibition of purple colored violacein.…”

Section: Evaluation Of the Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

Attenuation of quorum sensing regulated virulence functions and biofilm of pathogenic bacteria by medicinal plant Artemisia annua and its phytoconstituent 1, 8‐cineole

Khan,

Shahid,

Celik

et al. 2023

Microscopy Res & Technique

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“…The minimum inhibitory concentration (MIC), of a benzimidazole compound is one that prevents bacterial growth. In each test, 96‐well TARSONS plates were filled with 100 mL of sterile Muller‐Hinton Broth (MHB) [46] . Erythromycin, levofloxacin, and fluconazole that are available over the counter were diluted to concentrations of 512, 256, 128, 64, 32, 16, 8, 4, and 1 mg/mL and added to wells of benzimidazole compounds ( 3a – l ).…”

Section: Methodsmentioning

confidence: 99%

“…In each test, 96well TARSONS plates were filled with 100 mL of sterile Muller-Hinton Broth (MHB). [46] Erythromycin, levofloxacin, and fluconazole that are available over the counter were diluted to concentrations of 512, 256, 128, 64, 32, 16, 8, 4, and 1 mg/mL and added to wells of benzimidazole compounds (3a-l). Following the first ten wells in each row, a positive control containing organisms was placed in the eleventh well, and a negative control containing no organisms was placed in the twelfth well.…”

Section: Determination Of Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

Facile Synthesis of Benzimidazoles via Oxidative Cyclization of Acyclic Monoterpene Aldehyde with Diamines: Studies on Antimicrobial and in Vivo Evaluation of Zebrafish

Vaithiyalingam,

Mohan Kumar,

Kamaraj

et al. 2023

Chemistry & Biodiversity

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Citral (1a), a bioactive component of Cymbopogon citratus (lemongrass) could be isolated and semi‐synthetic analogs synthesized with improved therapeutic properties. Herein we first report describes citral (1a) as a primary material for the synthesis of benzimidazole derivatives between various o‐phenylenediamines (2a–l) in the presence of Diisopropylethylamine (DIPEA) as a commercially available environmentally benign base, ethanol as a green solvent and the yield of all benzimidazole derivatives (3a–l) was between 68–76 %; The semi‐synthetically prepared benzimidazole derivatives (3a–l) were assessed for their anti‐bacterial and anti‐fungal properties. The benzimidazole compounds (3a–b, and 3g–j) exhibit good anti‐microbial activity. In addition, in silico study was carried out to determine the specific binding affinity of the diamine halogen substituted benzimidazole derivatives to the specific target proteins. In silico analysis revealed a high correlation between docking results and experimental results. Finally, benzimidazole demonstrated significant antibacterial and antifungal activity. Zebrafish embryos were subjected to In vivo toxicological test found that all of the benzimidazole compounds (3a–l) were non‐toxic and had low embryotoxicity after 96 h, with an LC50 of 36.425 μg, which could facilitate the design of novel antimicrobial agents using a cost‐effective method.

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“…In contrast, Paudel et al [ 85 ] evaluated the antimicrobial activity of the essential oil of the aerial parts of C. asiatica and it did not show bioactivity against Bacillus cereus , Staphylococcus aureus , Escherichia coli , Pseudomonas aeruginosa and Aspergillus niger . Recently, Khan et al [ 86 ] also verified the activity of extracts of C. asiatica leaves and in-vitro leaf-calli using solvents methanol and ethyl acetate and observed that extracts showed antibacterial activity against C. violaceum 12742 and P. aeruginosa PAO1.…”

Section: Centella Asiatica Antimicrobial Activitymentioning

confidence: 99%

Centella asiatica and Its Metabolite Asiatic Acid: Wound Healing Effects and Therapeutic Potential

et al. 2023

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An intense effort has been focused on new therapeutic approaches and the development of technologies for more efficient and rapid wound healing. The research for plants used for long time in traditional medicine in the treatment of wound has become a promising strategy to obtain drugs therapeutically useful in the acute and chronic wound management. In this context, Centella asiatica (Apiaceae) has been used to treat a variety of skin diseases, such as leprosy, lupus, varicose ulcers, eczema and psoriasis, in Asiatic traditional medicine for thousands of years. Studies have shown that Centella asiatica extracts (CAE) display activity in tissue regeneration, cell migration and wound repair process by promoting fibroblast proliferation and collagen synthesis. Preliminary findings have shown that the asiatic acid is one of the main active constituents of C. asiatica, directly associated with its healing activity. Thus, this study discusses aspects of the effects of Centella asiatica and its active component, asiatic acid, in different stages of the healing process of cutaneous wounds, including phytochemical and antimicrobial aspects that contribute to its therapeutic potential.

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Anti-quorum sensing, antibiofilm, and antibacterial activities of extracts of Centella asiatica L. leaves, and in vitro derived leaves-calli through tissue culture: a potential for biofouling-prevention

Cited by 18 publications

References 42 publications

Attenuation of quorum sensing regulated virulence functions and biofilm of pathogenic bacteria by medicinal plant Artemisia annua and its phytoconstituent 1, 8‐cineole

Attenuation of quorum sensing regulated virulence functions and biofilm of pathogenic bacteria by medicinal plant Artemisia annua and its phytoconstituent 1, 8‐cineole

Facile Synthesis of Benzimidazoles via Oxidative Cyclization of Acyclic Monoterpene Aldehyde with Diamines: Studies on Antimicrobial and in Vivo Evaluation of Zebrafish

Centella asiatica and Its Metabolite Asiatic Acid: Wound Healing Effects and Therapeutic Potential

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