Anti-rheumatic activity of topical nanoemulsion containing bee venom in rats

Yousefpoor, Yaser; Amani, Amir; Divsalar, Adeleh; Mousavi, Sayed Mostafa Modarres; Shakeri, Abbas; Sabzevari, Javad Torkamannejad

doi:10.1016/j.ejpb.2022.02.005

Cited by 19 publications

(14 citation statements)

References 51 publications

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“…Our previous studies found a signi cant effect of NEs containing BV on improving arthritis in an animal model [13,14]. We did not know how much BV could have adverse effects after entering the body nor did we know its bene ts.…”

Section: Discussionmentioning

confidence: 95%

Topical bee venom nanoemulsion in collagen-induced arthritis model: effects on biochemical and hematological parameters

Yousefpoor

Amani

Hashemi

et al. 2023

Preprint

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Background: Traditionally, Bee venom (BV) is used through stinging or injection to treat rheumatoid arthritis (RA). This study aimed to assess the side effects of bee venom nanoemulsions (BV-NEs) in the collagen-induced arthritis (CIA) model by examining biochemical and hematological parameters. Methods: The BV-NEs were prepared, and the CIA model was induced in rats. After the seventh day, the groups were topically treated for two weeks as the following: blank (free treatment), negative control (NE-0), positive control (hydrocortisone acetate ointment 1%, 50 mg/day), BV control (37.5 µg/ml/day), and BV-NEs receiving 75, 37.5, 18.75, and 9.37 µg/ml/day. Three steps of blood sampling were done on days 0, 7, and 21 (healthy rats, before and at the end of treatment, respectively). Results: The results revealed that blood levels of glucose, cholesterol, urea, aspartate aminotransferase (AST), alkaline phosphatase (ALP), white blood cell (WBC), and %neutrophil significantly increased before the treatment. Nevertheless, most parameters declined at the end of the treatment compared to the blank and negative control groups about BV-NEs dose-dependently. The drastic changes in biochemical parameters in the CIA model indicated the effect of the immune system function on the metabolic system. Also, NE's impact of BV passed through the skin on these items. Conclusions: BV-NEs can reduce inflammation caused by arthritis without acute adverse effects on the routine biochemical and hematological parameters.

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Section: Discussionmentioning

confidence: 95%

Topical bee venom nanoemulsion in collagen-induced arthritis model: effects on biochemical and hematological parameters

Yousefpoor

Amani

Hashemi

et al. 2023

Preprint

Self Cite

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“…For example, cytolytic peptides increase penetration of BV into the deeper layers of skin after biting; these actions can differ depending on their amount [ 14 ]. For example, melittin shows an anti-inflammatory effect in minimal doses; however, it possesses an inflammatory effect in higher doses [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Subcutaneous Injection of Bee Venom in Wistar Rats: effects on blood cells and biochemical parameters

et al. 2022

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Objectives Bee venom (BV) therapy is performed by a bee sting or subcutaneous injection of BV. However, there is not much information on the effect of BV on blood parameters after entering the body. This project aimed to assess the side effects of subcutaneous BV injections in healthy rats by measuring the hematological and biochemical parameters. Methods Various amounts of BV, including 100, 200, and 500 (µg/day), were subcutaneously injected into rats for 30 days. The results showed that BV affected the metabolism of the liver, kidney, and glands. Results An increase in blood sugar and a decrease in other biochemical parameters, including cholesterol, triglyceride, urea, creatinine AST, ALT, ALP, and phosphorous, were observed. Results also showed increased counts of white blood cells, neutrophils (%), and platelets and decreased levels of red cells, hemoglobin, and hematocrit. Conclusion This study demonstrates that BV therapy in medical clinics requires routine care and testing to prevent eventual metabolic and anemia side effects.

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“…Nowadays, it is generally accepted that formulating the EOs in nanoemulsion and nanogel dosage forms improves their stability and efficacy [15,16]. Here, for the first time, the larvicidal effects of a nanogel (containing A. dracunculus EO) were investigated against A. stephensi and compared to its nanoemulsion.…”

Section: Introductionmentioning

confidence: 99%

Nanoemulsion and nanogel containing Artemisia dracunculus essential oil; larvicidal effect and antibacterial activity

et al. 2022

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Objective Microbial infections and mosquito-borne diseases such as malaria, with 627 k deaths in 2020, are still major public health challenges. Results This study prepared nanoemulsion and nanogel containing Artemisia dracunculus essential oil. ATR-FTIR analysis (Attenuated Total Reflection-Fourier Transform InfraRed) confirmed the successful loading of the essential oil in nanoemulsion and nanogel. LC50 values (Lethal Concentration 50%) of nanogel and nanoemulsion against Anopheles stephensi larvae were obtained as 6.68 (2–19 µg/mL) and 13.53 (7–25 µg/mL). Besides, the growth of Staphylococcus aureus after treatment with 5000 μg/mL nanogel and nanoemulsion was reduced by ~ 70%. However, about 20% growth of Pseudomonas aeruginosa was reduced at this dose. Considering the proper efficacy of the nanogel as a larvicide and proper antibacterial effect against S. aureus, it could be considered for further investigations against other mosquitoes’ larvae and gram-positive bacteria.

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Anti-rheumatic activity of topical nanoemulsion containing bee venom in rats

Cited by 19 publications

References 51 publications

Topical bee venom nanoemulsion in collagen-induced arthritis model: effects on biochemical and hematological parameters

Topical bee venom nanoemulsion in collagen-induced arthritis model: effects on biochemical and hematological parameters

Subcutaneous Injection of Bee Venom in Wistar Rats: effects on blood cells and biochemical parameters

Nanoemulsion and nanogel containing Artemisia dracunculus essential oil; larvicidal effect and antibacterial activity

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