Anti-rheumatic drugs and cartilage

Ghosh, Peter

doi:10.1016/s0950-3579(88)80017-7

Cited by 24 publications

(10 citation statements)

References 108 publications

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“…Aliquots (20 l 3 COOH, 10% ethanol) for 1 h and destained for 20 min in destain solution. The strips were further destained twice each for 1 h using fresh destain solution and dried overnight between paper towels.…”

Section: Alcian Blue Staining On Cellulose Acetate Stripsmentioning

confidence: 99%

“…The test solvent (1 ml) was added to the washed precipitate, the tubes were vortexed for 1 min and centrifuged for 20 min, at 9000 rpm and room temperature. The amount of 3 H radioactivity in the supernatant was determined by scintillation counting and expressed as the percentage of total radioactivity in [ 3 H]-PG precipitated with Alcian blue.…”

Section: Determination Of Solubility Of Alcian Blue-pg Complexmentioning

confidence: 99%

“…(1) for review). PG of articular cartilage is synthesized by articular chondrocytes (AC) and the rate of this synthesis has been found to be affected by several physiological factors (2) and therapeutic agents (3). Cultures of AC provide appropriate model system for the investigation of PG biosynthesis and its regulation by endogenous and exogenous biological factors and drugs.…”

mentioning

confidence: 99%

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Differential Use of Alcian Blue and Toluidine Blue Dyes for the Quantification and Isolation of Anionic Glycoconjugates from Cell Cultures: Application to Proteoglycans and a High-Molecular-Weight Glycoprotein Synthesized by Articular Chondrocytes

Terry

Chopra

Ovenden

et al. 2000

Analytical Biochemistry

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Section: Alcian Blue Staining On Cellulose Acetate Stripsmentioning

confidence: 99%

Section: Determination Of Solubility Of Alcian Blue-pg Complexmentioning

confidence: 99%

mentioning

confidence: 99%

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Differential Use of Alcian Blue and Toluidine Blue Dyes for the Quantification and Isolation of Anionic Glycoconjugates from Cell Cultures: Application to Proteoglycans and a High-Molecular-Weight Glycoprotein Synthesized by Articular Chondrocytes

Terry

Chopra

Ovenden

et al. 2000

Analytical Biochemistry

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“…Studies with animal cartilage have shown that some NSAIDs have catabolic influences [5] and others have anabolic influences [6]. This indicates that the effects of NSAIDs are independent of the inhibition of the prostaglandin pro- duction by chondrocytes [4,7]. Thus it is of great importance to know which NSAIDs have an anabolic action on cartilage.…”

Section: Introductionmentioning

confidence: 99%

In vitro influence of ketoprofen on the proteoglycan metabolism of human normal and osteoarthritis cartilage

Wilbrink¹,

Veen²,

Huber³

et al. 1991

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We investigated the influences of ketoprofen on the proteoglycan (PG) turnover of human articular cartilage explants in three groups: normal young with a high basal PG synthesis, normal adult and osteoarthritis cartilage, both with a low basal PG synthesis. Ketoprofen had no influence on the mean PG synthesis rate of normal adult and OA cartilage after 4 days of culture nor on the cartilage PG content after 8 days of culture. There was no relation between the histological grade of OA and effects of ketoprofen. In normal young cartilage ketoprofen induced an increase of the PG synthesis rate when added to the culture in a concentration of 10(-4) M. No correlation existed between the effect of ketoprofen and the basal PG synthesis of normal cartilage.

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“…Some laboratory and clinical evidence9 [11][12][13][14] suggests that several of the older NSAIDs may suppress the biosynthesis of proteoglycans in cartilage, thereby accelerating its failure rate. On the other hand, other data indicate that the effect of certain NSAIDs on cartilage is innocuous at therapeutically used concentrations, '5 16 or that these drugs may even provide a protective role by supporting macromolecule biosynthesis within joint tissues.…”

mentioning

confidence: 99%

Effects of tiaprofenic acid (Surgam) on cartilage proteoglycans in the rabbit joint immobilisation model.

Meyer-Carrive

Ghosh²

1992

Annals of the Rheumatic Diseases

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A well established model of arthritis induced in rabbit knee joints by immobilisation in full extension for 30 days was used to evaluate the in vivo effects of 2-5, 5-0, and 10-0 mg/kg body weight of tiaprofenic acid on articular cartilage proteoglycans. The drug was given subcutaneously every 24 hours during the entire immobilisation period. Immobilised animals not treated with drugs and normal animals were used as controls. In the non-drug treated immobilised animals articular cartilage showed evidence of surface damage accompanied by synovial hypertrophy and effusion. Proteoglycan concentrations were reduced in cartilages of these joints and the incorporation of 35SO24 into macromolecular proteoglycans was higher than in cartilages of non-immobilised controls. Gel filtration chromatographic studies of both resident and 35S labelled proteoglycans isolated from immobilised joint cartilage showed reduced aggregation and the presence of degraded proteoglycan subunit species. Whereas the administration of 10,0 mg/kg tiaprofenic acid every 24 hours to immobilised animals exacerbated the degradation and loss of proteoglycans from joint cartilages, 5-0 mg/kg tiaprofenic acid provided some protection of these macromolecules, as shown by the concentrations and extractability of proteoglycans from cartilages, which were comparable with those from non-immobilised controls. A high incorporation of 35S into proteoglycans was demonstrated, together with reduced catabolism of proteoglycans, indicating preservation of chondrocyte anabolic activity. At a tiaprofenic acid dose of 2.5 mg/kg, however, no beneficial effects on cartilage proteoglycans could be shown.

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Anti-rheumatic drugs and cartilage

Cited by 24 publications

References 108 publications

Differential Use of Alcian Blue and Toluidine Blue Dyes for the Quantification and Isolation of Anionic Glycoconjugates from Cell Cultures: Application to Proteoglycans and a High-Molecular-Weight Glycoprotein Synthesized by Articular Chondrocytes

Differential Use of Alcian Blue and Toluidine Blue Dyes for the Quantification and Isolation of Anionic Glycoconjugates from Cell Cultures: Application to Proteoglycans and a High-Molecular-Weight Glycoprotein Synthesized by Articular Chondrocytes

In vitro influence of ketoprofen on the proteoglycan metabolism of human normal and osteoarthritis cartilage

Effects of tiaprofenic acid (Surgam) on cartilage proteoglycans in the rabbit joint immobilisation model.

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