Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection

Azevedo, Paulo Hernane Rabelo; Xavier, Marcelo Antônio Pascoal; Silva, Glenda Nicioli da; Costa, Priscilla Almeida da; Carneiro, Cláudia Martins; Filho, Geraldo Brasileiro

doi:10.1590/0037-8682-0011-2018

Cited by 5 publications

(2 citation statements)

References 28 publications

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“…Immunohistochemistry significantly improves the diagnosis of cutaneous leishmaniasis in histological sections and allows the patient's immune response to be evaluated [ 217 – 219 ]. In addition, immunohistochemistry has been useful in the diagnosis of toxoplasmosis [ 220 – 222 ], echinococcosis [ 223 , 224 ], malaria [ 225 ] and for the detection of Trypanosoma cruzi [ 226 , 227 ].…”

Section: Immunohistochemical Detection Of Protozoal Pathogensmentioning

confidence: 99%

Immunohistochemical diagnosis of human infectious diseases: a review

et al. 2022

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Background Immunohistochemistry (IHC) using monoclonal and polyclonal antibodies is a useful diagnostic method for detecting pathogen antigens in fixed tissues, complementing the direct diagnosis of infectious diseases by PCR and culture on fresh tissues. It was first implemented in a seminal publication by Albert Coons in 1941. Main body Of 14,198 publications retrieved from the PubMed, Google, Google Scholar and Science Direct databases up to December 2021, 230 were selected for a review of IHC techniques, protocols and results. The methodological evolutions of IHC and its application to the diagnosis of infectious diseases, more specifically lice-borne diseases, sexually transmitted diseases and skin infections, were critically examined. A total of 59 different pathogens have been detected once in 22 different tissues and organs; and yet non-cultured, fastidious and intracellular pathogens accounted for the vast majority of pathogens detected by IHC. Auto-IHC, incorporating patient serum as the primary antibody, applied to diseased heart valves surgically collected from blood culture-negative endocarditis patients, detected unidentified Gram-positive cocci and microorganisms which were subsequently identified as Coxiella burnetii, Bartonella quintana, Bartonella henselae and Tropheryma whipplei. The application of IHC to ancient tissues dated between the ends of the Ptolemaic period to over 70 years ago, have also contributed to paleomicrobiology diagnoses. Conclusion IHC plays an important role in diagnostic of infectious diseases in tissue samples. Paleo-auto-IHC derived from auto-IHC, is under development for detecting non-identified pathogens from ancient specimens.

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Section: Immunohistochemical Detection Of Protozoal Pathogensmentioning

confidence: 99%

Immunohistochemical diagnosis of human infectious diseases: a review

et al. 2022

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“…The T. cruzi detection in tissues is usually performed using histology [10], immunohistochemistry [11], electron microscopy [12] and PCR [13]. Compared to the traditional detection techniques, PCR demonstrates higher sensitivity and specificity.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of <em>Trypanosoma cruzi</em> from formalin fixed placentas and fetuses of Wistar rats

Costa

Pereira

Júnior

et al. 2019

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Chagas disease is a tropical disease, endemic of the Americas and caused by the protozoan Trypanosoma cruzi. The classic transmission route of T. cruzi is based on the contact with feces from Triatominae insects containing trypomastigotes forms of the parasite. T. cruzi is also spread by oral and congenital forms, besides blood donation and organ transplant transmission. The parasite has a tropism to several organs and tissues, including heart, brain, digestive tract, muscles, placenta, and fetuses. The determination of parasite burden in organs is an important parameter for scientific assays related to parasite control and epidemiology. Among the procedures to detect and quantify T. cruzi, PCR demonstrates high sensitivity and specificity. In this study, we developed a simple and fast method to detect T. cruzi from fixed placentas and fetuses of Wistar rats. T. cruzi was detected only in tissues from infected animals, with different parasite burdens. Once the histology usually demonstrates some limitations such as low sensitivity and specificity to detect T. cruzi in fetuses, our methodology will be useful for fixed fetal tissues from organ banks. The detection of T. cruzi from gestational tissues will contribute to the elucidation of mechanisms related to congenital Chagas disease.

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