Anti-snake venom activities of ethanolic extract of fruits of Piper longum L. (Piperaceae) against Russell's viper venom: Characterization of piperine as active principle

Shenoy, Priyank; Nipate, Sonali S.; Sonpetkar, J.M.; Salvi, Nitin; Waghmare, A.B.; Chaudhari, Pravin D.

doi:10.1016/j.jep.2013.03.022

Cited by 31 publications

(22 citation statements)

References 47 publications

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“…(Mors et al, 2000). The effective venom neutralizing effect of Pioer longum fruit against Russell 's viper venom was attributed to the presence of piperine (Shenoy et al, 2013). The high flavonoids and alkaloids contents of the extract may partly contribute to the anti-venom activity of the extract, since they have been reported to possess protein binding and enzyme inhibiting properties, and also inhibit snake venom phospholipase A2 (PLA2) activity (Santosh et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Anti-snake Venom Activity of Leaf Extract of Sansevieria liberica ger.& labr (Agavaceae.) in Mice

Akah¹,

Nwagu²,

Oforkansi³

2019

ijSciences

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The prevalence of snake bite and death from snake bite envenomation is becoming a serious public health problem globally. The cost, non availability and the adverse reactions associated with antisnake venom serum has favoured the use of medicinal herbs by traditional healers in the treatment of snakebites. In this study we evaluated the antisnake venom activity of the leaves of Sansevieria liberica against Naja naja nigricollis venom in mice. S. liberica is very popular among the traditional healers in south east Nigeria for the treatment of snake bites. The ground fresh leaves were extracted with ethanol and a portion of the ethanol extract was fractionated with n-hexane, ethyl acetate and butanol to afford the respective solvent fractions. The extract was subjected to acute toxicity (LD 50) and phytochemical testing. The LD 50 of the venom was similarly determined, The antisnake venom activity of the extract/fraction was determined against the LD 50 (353.5 ug/kg) and double the LD 50 (707 ug/kg) of the venom. The effect of the extract on bleeding and clotting time of the venom-intoxicated mice was investigated. Also studied was the effect of the extract on acetylcholine-induced contraction of the isolated frog rectus abdominus. The extract/fractions significantly protected the mice from Naja naja nigricollis venom-induced mortality in mice. The bleeding and clotting time of the venom-intoxicated rats were significantly (p < 0.05) decreased by the extract/fractions. The acetylcholine-induced contraction of frog rectus abdominus was significantly inhibited by the extract. These results suggest that the leaves of S. liberica exhibit antisnake venom properties that could be harnessed in treating patients with snakebite envenomation.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Anti-snake Venom Activity of Leaf Extract of Sansevieria liberica ger.& labr (Agavaceae.) in Mice

Akah¹,

Nwagu²,

Oforkansi³

2019

ijSciences

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“…Piperine from Piper nigrum inhibits the adhesion of neutrophils to endothelial monolayers. Also it possesses inhibitory activities on prostaglandin and leukotrienes and thus possesses anti-inflammatory activity [ 149 – 151 ]. Quercetin is a potent inhibitor of lipoxygenase, and free quercetin and its glycosides rutin are present in Allium cepa skins [ 152 ].…”

Section: Discussionmentioning

confidence: 99%

Ethnopharmacological Survey of Medicinal Plants Used by Traditional Healers and Indigenous People in Chittagong Hill Tracts, Bangladesh, for the Treatment of Snakebite

Kadir

Karmoker

Alam

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Snakebites are common in tropical countries like Bangladesh where most snakebite victims dwell in rural areas. Among the management options after snakebite in Bangladesh, snake charmers (Ozha in Bengali language) are the first contact following a snakebite for more than 80% of the victims and they are treated mostly with the help of some medicinal plants. Our aim of the study is to compile plants used for the treatment of snakebite occurrence in Bangladesh. The field survey was carried out in a period of almost 3 years. Fieldwork was undertaken in Chittagong Hill Tracts, Bangladesh, including Chittagong, Rangamati, Bandarban, and Khagrachari. Open-ended and semistructured questionnaire was used to interview a total of 110 people including traditional healers and local people. A total of 116 plant species of 48 families were listed. Leaves were the most cited plant part used against snake venom. Most of the reported species were herb in nature and paste mostly used externally is the mode of preparation. The survey represents the preliminary information of certain medicinal plants having neutralizing effects against snake venoms, though further phytochemical investigation, validation, and clinical trials should be conducted before using these plants as an alternative to popular antivenom.

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“…This is interesting since it is often discussed in the literature that several antiophidic plants did not neutralize snake venoms per se, but could have antiophidic use once they could relieve some of the symptoms of snake envenoming, especially the local effects. It is related that the presence of tranquilizing, antioxidant, immunostimulating, and/or anti-inflammatory activities in certain plants could be of great interest in the alleviation of snake envenoming symptoms [ 103 , 104 ]. For example, some studies have shown that anti-inflammatory drugs could inhibit the edematogenic and other snake venom effects related to inflammation, such as necrosis and myotoxicity, induced by Bothrops venoms [ 105 , 106 ].…”

Section: Antivenom Activities Of Extracts Of Medicinal Plants Agaimentioning

confidence: 99%

Medicinal Plants for the Treatment of Local Tissue Damage Induced by Snake Venoms: An Overview from Traditional Use to Pharmacological Evidence

Félix-Silva

Silva-Júnior

Zucolotto

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Snakebites are a serious problem in public health due to their high morbimortality. Most of snake venoms produce intense local tissue damage, which could lead to temporary or permanent disability in victims. The available specific treatment is the antivenom serum therapy, whose effectiveness is reduced against these effects. Thus, the search for complementary alternatives for snakebite treatment is relevant. There are several reports of the popular use of medicinal plants against snakebites worldwide. In recent years, many studies have been published giving pharmacological evidence of benefits of several vegetal species against local effects induced by a broad range of snake venoms, including inhibitory potential against hyaluronidase, phospholipase, proteolytic, hemorrhagic, myotoxic, and edematogenic activities. In this context, this review aimed to provide an updated overview of medicinal plants used popularly as antiophidic agents and discuss the main species with pharmacological studies supporting the uses, with emphasis on plants inhibiting local effects of snake envenomation. The present review provides an updated scenario and insights into future research aiming at validation of medicinal plants as antiophidic agents and strengthens the potentiality of ethnopharmacology as a tool for design of potent inhibitors and/or development of herbal medicines against venom toxins, especially local tissue damage.

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Anti-snake venom activities of ethanolic extract of fruits of Piper longum L. (Piperaceae) against Russell's viper venom: Characterization of piperine as active principle

Cited by 31 publications

References 47 publications

Evaluation of the Anti-snake Venom Activity of Leaf Extract of Sansevieria liberica ger.& labr (Agavaceae.) in Mice

Evaluation of the Anti-snake Venom Activity of Leaf Extract of Sansevieria liberica ger.& labr (Agavaceae.) in Mice

Ethnopharmacological Survey of Medicinal Plants Used by Traditional Healers and Indigenous People in Chittagong Hill Tracts, Bangladesh, for the Treatment of Snakebite

Medicinal Plants for the Treatment of Local Tissue Damage Induced by Snake Venoms: An Overview from Traditional Use to Pharmacological Evidence

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