Anti-snake venom effect of secodolastane diterpenes isolated from Brazilian marine brown alga Canistrocarpus cervicornis against Lachesis muta venom

Domingos, Thaisa Francielle Souza; Vallim, Magui Aparecida; Sanchez, Eládio Flores; Teixeira, Valéria Laneuville; Fuly, André Lopes

doi:10.1590/s0102-695x2011005000048

Cited by 12 publications

(10 citation statements)

References 21 publications

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“…The diterpenes (87 µg/g) did not inhibit edematogenic activity of B. jararaca venom (at 2 µg/g, data not shown); and thus, would not prevent inflammation induced by snake bites. On the other hand, in our previous work, this mixture of diterpenes inhibited hemolysis and edema induced by a PLA 2 isolated from L. muta [21]. Despite the similarity in composition between the B. jararaca and L. muta venoms, the presence of specific components in each venom may explain the different inhibitory profiles of the diterpenes.…”

Section: Resultsmentioning

confidence: 76%

“…In Brazil, the brown marine algae are the most widely studied, with more than 300 diterpenes isolated from at least 35 species all over the world [19]. Although some biological activities have been studied [21,25,26,27], the potential of brown seaweeds to neutralize the toxic effects of snake venoms has not been widely investigated.…”

Section: Resultsmentioning

confidence: 99%

“…A variety of terpenes have been isolated from Dictyotacea species marine brown algae [19], and some pharmacological and ecological activities of dolastane and secodolastane diterpenes isolated from C. cervicornis (formerly Dictyota cervicornis ) have been investigated [16,20]. In our previous work, we showed that dolastane and two secodolastane diterpenes (linearol/isolinearol) isolated from the marine brown alga, C. cervicornis , displayed antivenom effects against some toxic activities of Lacheis muta [16,21]. In the present work, we evaluated the effect of these two secodolastane diterpenes isolated from the same alga to inhibit some in vitro (hemolysis, proteolysis and clotting) and in vivo (hemorrhage, edema and lethal) activities of B. jararaca venom.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Diterpenes Isolated of the Marine Alga Canistrocarpus cervicornis against Some Toxic Effects of the Venom of the Bothrops jararaca Snake

et al. 2015

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Snake venoms are composed of a complex mixture of active proteins and peptides which induce a wide range of toxic effects. Envenomation by Bothrops jararaca venom results in hemorrhage, edema, pain, tissue necrosis and hemolysis. In this work, the effect of a mixture of two secodolastane diterpenes (linearol/isolinearol), previously isolated from the Brazilian marine brown alga, Canistrocarpus cervicornis, was evaluated against some of the toxic effects induced by B. jararaca venom. The mixture of diterpenes was dissolved in dimethylsulfoxide and incubated with venom for 30 min at room temperature, and then several in vivo (hemorrhage, edema and lethality) and in vitro (hemolysis, plasma clotting and proteolysis) assays were performed. The diterpenes inhibited hemolysis, proteolysis and hemorrhage, but failed to inhibit clotting and edema induced by B. jararaca venom. Moreover, diterpenes partially protected mice from lethality caused by B. jararaca venom. The search for natural inhibitors of B. jararaca venom in C. cervicornis algae is a relevant subject, since seaweeds are a rich and powerful source of active molecules which are as yet but poorly explored. Our results suggest that OPEN ACCESSMolecules 2015, 20 3516 these diterpenes have the potential to be used against Bothropic envenomation accidents or to improve traditional treatments for snake bites.

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Section: Resultsmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Diterpenes Isolated of the Marine Alga Canistrocarpus cervicornis against Some Toxic Effects of the Venom of the Bothrops jararaca Snake

et al. 2015

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“…A growing number of studies have been focused on the search for inhibitors of snake venoms from a variety of sources, be they natural or synthetic [31, 32]. Suramin [33–35] and benzoyl phenyl benzoate [36] are synthetic molecules able to inhibit myotoxicity, clotting, and phospholipase A 2 and hyaluronidase activities of snake venoms from different families.…”

Section: Resultsmentioning

confidence: 99%

Antivenom Effects of 1,2,3-Triazoles againstBothrops jararacaandLachesis mutaSnakes

Domingos

Moura

Campos

et al. 2013

BioMed Research International

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Snake venoms are complex mixtures of proteins of both enzymes and nonenzymes, which are responsible for producing several biological effects. Human envenomation by snake bites particularly those of the viperid family induces a complex pathophysiological picture characterized by spectacular changes in hemostasis and frequently hemorrhage is also seen. The present work reports the ability of six of a series of 1,2,3-triazole derivatives to inhibit some pharmacological effects caused by the venoms of Bothrops jararaca and Lachesis muta. In vitro assays showed that these compounds were impaired in a concentration-dependent manner, the fibrinogen or plasma clotting, hemolysis, and proteolysis produced by both venoms. Moreover, these compounds inhibited biological effects in vivo as well. Mice treated with these compounds were fully protected from hemorrhagic lesions caused by such venoms. But, only the B. jararaca edema-inducing activity was neutralized by the triazoles. So the inhibitory effect of triazoles derivatives against some in vitro and in vivo biological assays of snake venoms points to promising aspects that may indicate them as molecular models to improve the production of effective antivenom or to complement antivenom neutralization, especially the local pathological effects, which are partially neutralized by antivenoms.

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“…Previous studies showed the ability of dolastane and secodolastane diterpenes isolated from C. cervicornis marine brown alga to inhibit hemolytic, proteolytic, hemorrhagic and clotting activities of L. muta venom (Moura et al, 2010;Domingos et al, 2011). We have now evaluated the ability of S. schröederi algal extracts prepared with solvents of increasing polarities (n-Hexane, dichloromethane, ethyl acetate) to neutralize the hemolytic, proteolytic, hemorrhagic and clotting activities of crude L. muta venom.…”

Section: Resultsmentioning

confidence: 99%

Inhibitory effect of a Brazilian marine brown alga Spatoglossum schröederi on biological activities of Lachesis muta snake venom

Domingos¹,

Ortiz-Ramírez²,

Villaça³

et al. 2012

Rev. bras. farmacogn.

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Abstract:The ability of crude extracts of the brown seaweed Spatoglossum schröederi to counteract some of the biological activities of Lachesis muta snake venom was evaluated. In vitro assays showed that only the extract of S. schröederi prepared in ethyl acetate was able to inhibit the clotting of fibrinogen induced by L. muta venom. On the other hand, all extracts were able to inhibit partially the hemolysis caused by venom and those prepared in dichloromethane or ethyl acetate fully neutralized the proteolysis and hemorrhage produced by the venom. Moreover, the dichloromethane or ethyl acetate extracts inhibited the hemolysis induced by an isolated phospholipase A 2 from L. muta venom, called LM-PLA 2 -I. In contrast, the hexane extract failed to protect mice from hemorrhage or to inhibit proteolysis and clotting. These results show that the polarity of the solvent used to prepare the extracts of S. schröederi algae influenced the potency of the inhibitory effect of the biological activities induced by L. muta venom. Thus, the seaweed S. schröederi may be a promising source of natural inhibitors of the enzymes involved in biological activities of L. muta venom.

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Anti-snake venom effect of secodolastane diterpenes isolated from Brazilian marine brown alga Canistrocarpus cervicornis against Lachesis muta venom

Cited by 12 publications

References 21 publications

Effect of Diterpenes Isolated of the Marine Alga Canistrocarpus cervicornis against Some Toxic Effects of the Venom of the Bothrops jararaca Snake

Effect of Diterpenes Isolated of the Marine Alga Canistrocarpus cervicornis against Some Toxic Effects of the Venom of the Bothrops jararaca Snake

Antivenom Effects of 1,2,3-Triazoles againstBothrops jararacaandLachesis mutaSnakes

Inhibitory effect of a Brazilian marine brown alga Spatoglossum schröederi on biological activities of Lachesis muta snake venom

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