Anti-spasmolytic and Anti-diarrheal Activities of Newbouldia laevis, Cola nitida and Acanthus montanus Extracts on Gastrointestinal Muscles

Ibeh, Raymond C.; Ikechukwu, Gavin C.; Ijioma, Solomon Nnah; Nwankwo, Chinedu Ifeanyi; Singh, Ashok Kumar; Samani, Majid Asadi; Onoja, Samuel O.; Aloh, G. S.

doi:10.3923/jpt.2019.1.8

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…The autonomic nervous system acts via the release of norepinephrine and adrenaline to inhibit intestinal contractions ( 22 , 23 , 24 ) by the attachment of these neurotransmitters to the adrenergic receptors, all coupled to trimeric G proteins ( 25 ). We wanted to determine if ACAE acts via α and β adrenergic receptors.…”

Section: Discussionmentioning

confidence: 99%

Myorelaxant and antispasmodic effect of an aqueous extract of <i>Artemisia campestris</i> L. via calcium channel blocking and anticholinergic pathways

Marghich

Aziz

Mekhfi

et al. 2021

J. Smooth Muscle Res.

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Intestinal spasms are violent contractions that occur in the intestine, which cause discomfort to people who have them. Medicinal plants are widely used in traditional Moroccan medicine to treat these problems, among these being Artemisia campestris L. This study aims to evaluate the relaxant and antispasmodic effects of an aqueous extract of this plant (ACAE). It was performed in vitro on isolated segments of both isolated rat and rabbit jejunum mounted in an organ bath and tension recordings made via an isotonic transducer. ACAE caused a myorelaxant effect on baseline rabbit jejunum contractions in a dose-dependent and reversible manner with an IC 50 of 1.52 ± 0.12 mg/ml. This extract would not act via adrenergic receptors pathway. On the other hand, the extract caused a dose-dependent relaxation of the jejunum tone in rat jejenum segments pre-contracted with either Carbachol (CCh; 10 −6 M) or high K + (KCl 75 mM) with an IC 50 = 0.49 ± 0.02 mg/ml and 0.36 ± 0.02 mg/ml respectively. In the presence of different doses of the extract, the maximum response to CCh and CaCl 2 was significantly reduced. This demonstrates that ACAE acts on both muscarinic receptors and voltage-dependent calcium channels. Thus, the plant extract acted on both muscarinic and nicotinic receptors and acts on the guanylate cyclase pathway, but not the nitric oxide pathway. These results indicate the mechanism by which Artemisia campestris L. acts as an effective antispasmodic agent in traditional Moroccan medicine.

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Section: Discussionmentioning

confidence: 99%

Myorelaxant and antispasmodic effect of an aqueous extract of <i>Artemisia campestris</i> L. via calcium channel blocking and anticholinergic pathways

Marghich

Aziz

Mekhfi

et al. 2021

J. Smooth Muscle Res.

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show abstract

“…One of the most common diseases in humans is gastrointestinal (GI) disorder [1][2][3][4]. A spasm is defined as an uncontrollable muscular contraction of the smooth muscles or a transitory constriction of a passage such as the intestine, bladder, lungs, or stomach that is usually accompanied by pain and can be induced by endogenous acetylcholine [5].…”

Section: Introductionmentioning

confidence: 99%

“…Antispasmodic agents, which relax and subdue muscle spasms or contractions, can counteract antagonistic muscarinic actions. They prevent stomach and intestine spasms by inhibiting cholinergic nerve impulses by blocking the action of acetylcholine neurotransmitters in the parasympathetic nervous system [4]. Antispasmodics are divided into two categories: anticholinergic (dicyclomine and hyoscyamine) and musculotropic (mebeverine).…”

Section: Introductionmentioning

confidence: 99%

Antispasmodic Effect of Alstonia boonei De Wild. and Its Constituents: Ex Vivo and In Silico Approaches

Akinmurele,

Sonibare,

Elujoba

et al. 2023

Molecules

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Background: Alstonia boonei, belonging to the family Apocynaceae, is one of the best-known medicinal plants in Africa and Asia. Stem back preparations are traditionally used as muscle relaxants. This study investigated the antispasmodic properties of Alstonia boonei Stem back and its constituents. Method: The freeze-dried aqueous Stem back extract of A. boonei, as well as dichloromethane (DCM), ethyl acetate, and aqueous fractions, were evaluated for their antispasmodic effect via the ex vivo method. Two compounds were isolated from the DCM fraction using chromatographic techniques, and their antispasmodic activity was evaluated. An in silico study was conducted by evaluating the interaction of isolated compounds with human PPARgamma-LBD and human carbonic anhydrase isozyme. Results: The Stem back crude extract, DCM, ethyl acetate, and aqueous fractions showed antispasmodic activity on high-potassium-induced (K+ 80 mM) contractions on isolated rat ileum with IC50 values of 0.03 ± 0.20, 0.02 ± 0.05, 0.03 ± 0.14, and 0.90 ± 0.06 mg/mL, respectively. The isolated compounds from the DCM fraction were β-amyrin and boonein, with only boonein exhibiting antispasmodic activity on both high-potassium-induced (IC50 = 0.09 ± 0.01 µg/mL) and spontaneous (0.29 ± 0.05 µg/mL) contractions. However, β-amyrin had a stronger interaction with the two proteins during the simulation. Conclusion: The isolated compounds boonein and β-amyrin could serve as starting materials for the development of antispasmodic drugs.

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Oral changes associated with kolanut use: a report of 2 cases

Ramil

Almazrooa²,

Binmadi³

et al. 2020

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

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Anti-spasmolytic and Anti-diarrheal Activities of Newbouldia laevis, Cola nitida and Acanthus montanus Extracts on Gastrointestinal Muscles

Cited by 3 publications

References 16 publications

Myorelaxant and antispasmodic effect of an aqueous extract of <i>Artemisia campestris</i> L. via calcium channel blocking and anticholinergic pathways

Myorelaxant and antispasmodic effect of an aqueous extract of <i>Artemisia campestris</i> L. via calcium channel blocking and anticholinergic pathways

Antispasmodic Effect of Alstonia boonei De Wild. and Its Constituents: Ex Vivo and In Silico Approaches

Oral changes associated with kolanut use: a report of 2 cases

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