2023
DOI: 10.1021/acsomega.3c04273
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Anti-SpCas9 IgY Polyclonal Antibodies Production for CRISPR Research Use

Esteban León,
Valentina Ortiz,
Alexander Pérez
et al.

Abstract: The CRISPR/Cas adaptative immune system has been harnessed as an RNA-guided, programmable genome editing tool, allowing for diverse biotechnological applications. The implementation of the system relies on the ability to detect the Cas9 protein in biological samples. This task is facilitated by employing antibodies, which exhibit several advantageous features and applications in the context of tropical neglected diseases. This study reports a one-month immunization scheme with the Cas9 protein fromStreptococcu… Show more

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