Anti-sperm antibodies and seminal characteristics after testicular biopsy or epididymal aspiration in dogs

Attia, Khaled Ahmed; Zaki, Abdel Kader A.; Eilts, B.E.; Paccamonti, Dale L.; Hosgood, Giselle; Dietrich, Mariola A.; Horohov, David W.; Blouin, D.

doi:10.1016/s0093-691x(00)00278-8

Cited by 29 publications

(18 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The testicular weight was estimated from the testis dimensions, once for this work samples were taken by testicular biopsy. Testicular biopsy is the elective procedure to study reproductive morphophysiology in wild animals (Azevedo et al, 2006;Guião-Leite et al, 2006;Sarti, 2006), as it is a conservative method that maintains the reproductive capacity, showing no harmful effects on sperm production in the long term (Lopate et al, 1989;Threlfall & Lopate, 1993;Attia et al, 2000). Unilateral testicular biopsy in dogs of undetermined breed (mongrel dog) showed no changes in the cell population, the intrinsic efficiency of the spermatogenic process and the Sertoli cell index, either in the biopsied testis or in the contralateral testis, after a period of 60 days (Mascarenhas et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Morphometry of testis and seminiferous tubules of the adult crab-eating fox (Cerdocyon thous, Linnaeus, 1766)

et al. 2010

View full text Add to dashboard Cite

RESUMO Morfometria testicular e de túbulos seminíferos do cachorro-do-mato (Cerdocyon thous, Linnaeus, 1766) adultoOs parâmetros de biometria corporal e testicular são de grande importância no estabelecimento dos padrões reprodutivos e, consequentemente, no desenvolvimento de protocolos para a reprodução assistida nas diferentes espécies. Uma correlação direta entre o peso do testículo e a população espermática é observada nas diferentes espécies já estudadas, uma vez que o tamanho do testículo reflete principalmente o volume total do túbulo seminífero, seu principal componente. O presente trabalho tem como objetivos definir parâmetros volumétricos testiculares e correlacionar os dados de morfometria testicular e do túbulo seminífero com a massa corporal em seis cachorros-domato adultos. O peso corporal médio dos cachorros-do-mato foi de 6,53 Kg, dos quais cerca de 0,068% são alocados em massa testicular e 0,042% especificamente em túbulos seminíferos, que representam 87,5% do parênquima testicular. A albugínea representa 12,5% da massa testicular. O diâmetro médio dos túbulos seminíferos em cachorro-do-mato foi de 236 μm, e a espessura média do epitélio seminífero foi de 62,9 μm, sendo encontrados em média 18 metros de túbulo seminífero por grama de testículo. Os valores para os parâmetros tubulares indicam uma produtividade espermática próxima àquelas observadas nos carnívoros já estudados. Body and testicular biometric parameters are very important for establishing reproductive patterns and, consequently, the development of protocols for assisted reproduction in different species. A direct correlation between the testis weight and the sperm population was observed in other studied species, because the testis size primarily reflects the total volume of the seminiferous tubule, its main component. The objective of this study was to determine the testicular volume parameters and correlate data from morphometry of testis and seminiferous tubules with body mass in six adult crab-eating foxes. The mean body weight of the crab-eating foxes in this study was 6.53 kg, with approximately 0.068% allocated to the testicular mass and 0.042% specifically to seminiferous tubules, which represented 87.5% of the testicular parenchyma. The albuginea comprised 12.5% of the testicular mass. The mean diameter of seminiferous tubules was 236 μm, and the mean thickness of the seminiferous epithelium was 62.9 μm. Values of tubular parameters indicate a sperm productivity close to those observed in previously studied carnivores. Palavras

show abstract

Section: Resultsmentioning

confidence: 99%

Morphometry of testis and seminiferous tubules of the adult crab-eating fox (Cerdocyon thous, Linnaeus, 1766)

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Attia et al . () found that one of three dogs that underwent epididymal aspiration developed a transient formation of anti‐sperm IgG on spermatozoa with no negative effect on total spermatozoa output or motility. Although this study concluded that epididymal aspiration is a safe procedure, side effects of PESA require further investigations.…”

Section: Discussionmentioning

confidence: 97%

Quality of canine spermatozoa retrieved by percutaneous epididymal sperm aspiration

Varesi

Vernocchi

Faustini

et al. 2013

J of Small Animal Practice

View full text Add to dashboard Cite

In case of ejaculation failure due to pathological conditions in dogs, the collection of spermatozoa from the cauda of the epididymis could be an option for providing gametes for assisted reproductive technologies. Percutaneous epididymal sperm aspiration can be used in dogs with compromised reproductive performance, in which orchiectomy cannot be performed for medical or owner reasons. Further studies aimed to investigate whether the percutaneous epididymal sperm aspiration technique might be feasible for repeated semen collection and to accurately evaluate side effects are required.

show abstract

“…The collection techniques are mainly eletroejaculation, rectal massage and artificial vagina, and the main cryoprotectants based on glycerol, with effective results in wild species such as impalas, giraffes, and buffaloes, among others. Testicular biopsy is an elective methodology for the collection of material for physiology and reproductive pathologies study in wildlife, providing enough material for histological analysis (Lopate et al 1989, Threlfall & Lopate 1993, Attia et al 2000, Crosta et al 2003, Azevedo et al 2006, Mascarenhaset al 2006, Guião--Leite et al 2006, Bitencourt al. 2007).…”

Section: Cryopreservation Of Spermatozoidsmentioning

confidence: 99%

Maintenance of Brazilian Biodiversity by germplasm bank

et al. 2016

View full text Add to dashboard Cite

Currently the importance of using alternative strategies for biodiversity conservation is emphasized and since the establishment of germplasm bank is an alternative to the conservation of endangered species. This is a technique of great importance for the maintenance of Brazilian fauna. Since the early70'sthere was a growing concern about the need to preserve essential genetic resources for food and agriculture, mainly for conservation of genetic material from farm animals. Thus was created the Brasilia Zoo, in July 2010, the first Germplasm Bank of Wild Animals in Latin America, as an alternative strategy for the conservation of threatened or endangered species, using both gametes and somatic cells and stem cells. Then we argue to create new banks or research networks among different regions with aimed to tissue preservation.

show abstract

Anti-sperm antibodies and seminal characteristics after testicular biopsy or epididymal aspiration in dogs

Cited by 29 publications

References 20 publications

Morphometry of testis and seminiferous tubules of the adult crab-eating fox (Cerdocyon thous, Linnaeus, 1766)

Morphometry of testis and seminiferous tubules of the adult crab-eating fox (Cerdocyon thous, Linnaeus, 1766)

Quality of canine spermatozoa retrieved by percutaneous epididymal sperm aspiration

Maintenance of Brazilian Biodiversity by germplasm bank

Contact Info

Product

Resources

About