Anti-sperm antibodies, detected by agglutination, immobilization, microcytotoxicity and immunobead-binding assays

Bronson, Richard; Cooper, George; Hjort, T; Ing, R. M. Y.; Jones, Warren R.; Wang, S.X.; Mathur, Subbi; Williamson, H. Oliver; Rust, Philip F.; Fudenberg, H. Hugh; Mettler, L.; Czuppon, A.B.; Sudo, Natsuru

doi:10.1016/0165-0378(85)90003-8

Cited by 65 publications

(17 citation statements)

References 14 publications

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“…Several immunoblotting assays earlier demonstrated the presence of ASA in a high proportion of fertile subjects [21,28,31]. ASA in a fertile population may therefore reflect a natural physiological phenomenon [21,28,46], and the occurrence of the antibodies to intracellular components seems to be relatively ubiquitous [7]. Several of these intracellular antigens have been microsequenced and identified as components of sperm specific structures such as the fibrous sheath and outer dense fibers (unpublished data).…”

Section: Discussionmentioning

confidence: 92%

Human Sperm Proteome: Immunodominant Sperm Surface Antigens Identified with Sera from Infertile Men and Women1

Shetty

Naaby-Hansen

Shibahara

et al. 1999

Biology of Reproduction

110

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The objective of this study was to identify those immunodominant sperm antigens recognized by antisperm antibodies (ASA) in the serum samples of infertile men and women. High-resolution two-dimensional gel electrophoresis was employed to separate human sperm proteins using isoelectric focusing or nonequilibrium pH gradient electrophoresis, followed by PAGE. Serum samples from 15 infertile male subjects and 6 infertile female subjects that contained ASA as assayed by the immunobead binding test (IBT) were analyzed by Western blotting followed by enhanced chemiluminescence (ECL). Serum samples from 10 fertile subjects (5 males and 5 females) that were ASA negative by IBT were used as controls. The ECL blots were analyzed by computer scanning to compare the immunoreactivity between serum samples from fertile and infertile subjects and to identify the antigens unique to the sera of the infertile subjects; 98 sperm auto- and iso-antigenic protein spots were recognized by sera from infertile males and females but not from fertile subjects. Based on vectorial labeling with 125I at the sperm surface, a subset of 6 auto- and iso-antigens was identified as possibly relevant to antibody-mediated infertility.

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Section: Discussionmentioning

confidence: 92%

Human Sperm Proteome: Immunodominant Sperm Surface Antigens Identified with Sera from Infertile Men and Women1

Shetty

Naaby-Hansen

Shibahara

et al. 1999

Biology of Reproduction

110

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“…Studies on anti-sperm antibodies in women have shown wide discrepancies between results from different laboratories. In most laboratories mainly low antibody titres have been recorded in female sera, and the clinical significance of such weak reactions is questionable because similar reactivities can be observed in control groups (Bronson et al, 1985). In women the interest is therefore now being focused on antibodies in cervical mucus.…”

Section: Detection and Occurrence Of Anti-sperm Antibodiesmentioning

confidence: 99%

Anti-sperm antibodies and immune subfertility

Hjort

Meinertz

1988

Human Reproduction

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A brief description of immune subfertility, caused by antibodies directed against sperm-specific glycoproteins in the sperm membrane, is given. Observations during the last few years have indicated that this immune disease is primarily caused by anti-sperm antibodies of the IgA class, and recent results from vasovasostomized men lend further support to this theory. Detection of anti-sperm antibodies should therefore include characterization of the immunoglobulin class of antibodies. In male patients this can be achieved by direct mixed anti-globulin reaction test or immunobead-binding test on the patients' spermatozoa, and in female patients cervical mucus can be tested by indirect versions of the same tests.

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“…14-26) and stored at -20°C until used. The fertile men did not reveal any antisperm antibodies, but the infertile men exhibited various titers of antisperm antibodies in their sera and/or on sperm when analyzed by the tray agglutination technique [lo], sperm immobilization technique [l 11, and immunobead technique [6]. The infertility of these men was attributed to immunologic factors and hence they are referred to as "immunoinfertile.…”

Section: Methodsmentioning

confidence: 99%

Enhanced Levels of Colony Stimulating Factor-1 (Csf-1) in Sera and Seminal Plasma of Antisperm Antibody-Positive Infertile Men

Naz

Stanley

1995

Archives of Andrology

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Anti-sperm antibodies, detected by agglutination, immobilization, microcytotoxicity and immunobead-binding assays

Cited by 65 publications

References 14 publications

Human Sperm Proteome: Immunodominant Sperm Surface Antigens Identified with Sera from Infertile Men and Women1

Human Sperm Proteome: Immunodominant Sperm Surface Antigens Identified with Sera from Infertile Men and Women1

Anti-sperm antibodies and immune subfertility

Enhanced Levels of Colony Stimulating Factor-1 (Csf-1) in Sera and Seminal Plasma of Antisperm Antibody-Positive Infertile Men

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