Anti-Toxoplasma activities of the hydroalcoholic extract of some brassicaceae species

Montazeri, Mahbobeh; Mirzaee, Fatemeh; Daryani, Ahmad; Naeimayi, Raheleh; Karimabad, Shohre Moradi; Arjmandi, Hadiseh Khalilzadeh; Esmaealzadeh, Niusha; Shahani, Somayeh

doi:10.4103/abr.abr_206_19

Cited by 12 publications

(10 citation statements)

References 27 publications

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“…Indeed, we can observe that Dichloromethane: Methanol extract and Dichloromethane extract of D. kotschyi had the highest Selectivity Indices, illustrating better selectivity, with low effect for host cell: 30.197 and 15.667, respectively. The data are consistent with the results of Montazerri et al [7] who also showed that Pyrimethamine was toxic to the host cell in comparison to Hydroalcoholic Extract of some Brassicaceae Species. This toxicity of the reference drugs is probably linked to their reduced efficacy reported in several studies, which requires the use of high concentrations that are, therefore, toxic for the cells.…”

Section: Discussionsupporting

confidence: 92%

“…Indeed, at the end of thein vitro investigations, it was observed that that Sulfadiazine (reference drugs and positive control) was the most toxic treatment for the host cells (SeIectivity Index = 1.552). Sulfadiazine is one of the molecules used in the treatment of toxoplasmosis [6,7]. It is, therefore, highly toxic to host cells.…”

Section: Discussionmentioning

confidence: 99%

“…This is the first report of the anti-Toxoplasma activity of D. kotschyi, but several data confirm the high activity and low toxicity of natural substances from medicinal plants against Toxoplasma. In a study dedicated to anti-Toxoplasma activity of some Brassicaceae, extracts of L. sativum, L. perfoliatum, N. officinale, A. homolocarpum, C. bursa-pastoris presented 50% inhibitory concentrations of 5.1, 14.67, 32.49, 37.31, 71.35 and selectivity indices of 8.06, 2.59, 0.74, 0.78, respectively, in comparison to pyrimethamine (IC 50 = 63 μg/ml) [7].…”

Section: Discussionmentioning

confidence: 99%

“…The therapeutic management of toxoplasmosis involves antiparasitic drugs, such as Sulfadiazine and Pyrimethamine, but problems of ineffectiveness, parasite resistance, toxicity, and side effects have been reported [6,7]. Among the possibilities to be explored or alternatives to antiparasitic drugs, natural substances from medicinal plants occupy a prominent place.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, several medicinal plants have been reported in the traditional treatment of toxoplasmosis. Also, several pharmacological studies demonstrated the ability of medicinal plant extracts to inhibit in vitro and in vivo the growth of T. gondii [1,[7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro and In Vivo Anti-Toxoplasma Activities of Dracocephalum kotschyi Extract in Experimental Models of Acute Toxoplasmosis

et al. 2021

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Background The problem of resistance to antiparasitic drugs, associated with their side effects, suggest exploring other alternatives, including medicinal plants. Dracocephalum kotschyi (D. kotschyi), for example, from Lamiaceae family, is a plant widely used in Iran and in many countries, and to which interesting pharmacological properties have been attributed. This study aimed to investigate in vitro and in vivo anti-Toxoplasma activities of D. kotschyi extract in experimental models of acute toxoplasmosis. Methods Anti-Toxoplasma activity of the extracts in vitro was performed on Vero Cell, using the MTT test. Vero cell were infected with (3 × 10 5 tachyzoites/well) following treatment with Dichloromethane (F1), dichloromethane: methanol (F2), methanol (F3), methanol: water (F4), and deionized water (F5) extracts of D. kotschyi, and pyrimethamine and sulfadiazine (positive control). MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was used to measure cell viability. Effects of extracts on tachyzoites viability were evaluated by trypan blue exclusion method, followed by light microscopy. For in vivo test, 18 groups of 8-10-week-old Inbred Balb/c mice weighing 18-20 g, were intraperitoneally infected with 2 × 10 3 tachyzoites and then treated with sterile PBS (negative control), pyrimethamine (25 mg/kg) and sulfadiazine (500 mg/kg) as positive controls, and F1 to F5 extracts (at doses 50, 100 and 200 mg/kg).

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro and In Vivo Anti-Toxoplasma Activities of Dracocephalum kotschyi Extract in Experimental Models of Acute Toxoplasmosis

et al. 2021

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Evaluation of the therapeutic effect of Descurainia sophia (L.) Webb ex Prantl seed extract on hyperthyroidism: A double‐blind placebo‐controlled pilot clinical trial

Farzameh,

Azadbakht,

Kashi

et al. 2023

Food Science & Nutrition

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The seeds of Descurainia sophia (L.) Webb ex Prantl contain goitrogenic glucosinolates, such as gluconapin (3‐butenyl glucosinolate). Because of the important role of iodine in the synthesis of thyroid hormones and the inhibitory activity of D. sophia on iodine uptake by the thyroid gland, this study aimed to determine the effects of D. sophia syrup on clinical and biochemical variables of thyrotoxicosis in hyperthyroid patients. In this randomized, double‐blind trial, 10 newly diagnosed hyperthyroid female patients were randomly assigned to treatment with (1) methimazole (MMI) plus D. sophia capsules (350 mg/d) or (2) MMI plus placebo capsules. The primary outcomes were clinical and biochemical manifestations of hyperthyroidism after 60 days. Serum levels of FT3 and FT4 significantly decreased (5.9 ± 2.5 vs. 9.4 ± 5.9 and 29.1 ± 3.1 vs. 31.77 ± 3.7, respectively) and the serum thyroid‐stimulating hormone (TSH) concentration significantly increased in the D. sophia group contrasted to the placebo group (4.7 ± 0.1 vs. 0.05 ± 0.02). Significant improvement in the thyrotoxicosis clinical symptoms was reported in the D. sophia group contrasted to the placebo group (p < .05). D. sophia can suppress plasma levels of FT3 and FT4 and may be useful as adjunctive therapy for hyperthyroidism.

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Antiprotozoal Activity of Secondary Metabolites

Gulsen,

Touray,

Cimen

et al. 2024

Reference Series in Phytochemistry

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Anti-Toxoplasma activities of the hydroalcoholic extract of some brassicaceae species

Cited by 12 publications

References 27 publications

In Vitro and In Vivo Anti-Toxoplasma Activities of Dracocephalum kotschyi Extract in Experimental Models of Acute Toxoplasmosis

In Vitro and In Vivo Anti-Toxoplasma Activities of Dracocephalum kotschyi Extract in Experimental Models of Acute Toxoplasmosis

Evaluation of the therapeutic effect of Descurainia sophia (L.) Webb ex Prantl seed extract on hyperthyroidism: A double‐blind placebo‐controlled pilot clinical trial

Antiprotozoal Activity of Secondary Metabolites

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