Anti tubercular minimum inhibitory concentration (MIC) and chemical characterization of ethnobotanical mixture used in the treatment of tuberculosis

Rastogi, Vijaylatha; Tomar, Jyoti; Patni, Tarun; Vijay, Chhavi; Sharma, Pramod Kumar

doi:10.18231/2394-5478.2019.0011

Cited by 3 publications

(6 citation statements)

References 8 publications

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“…This is of significance, as it elucidates the molecular basis of efficacy of Hawan medicinal fumes in the inhalational therapy of pulmonary tuberculosis as reported [8,33] in literature. Sig-nificant in-vitro tuberculocidal activity of this herbal mixture extract when tested against clinical Mycobacterium tuberculosis (Mtb) strains has also been reported [9] corroborating with the observations. This finding is thus of importance in the preparation of novel antitubercular compounds from Hawan ingredients and development of inhalational therapy.…”

Section: Discussionsupporting

confidence: 82%

“…GC-MS analysis of Hawan fumes and pyrolysis fumes of Hawan ingredients revealed presence of an array of bioactive compounds mainly n-Hexadecanoic acid, oleic acid, tetradecanoic acid, octadecanoic acid, 4-Methyl-2,5-dimethoxybenzaldehyde, 2-Methoxy-4-vinylphenol, coumarilic acid, eugenol, camphor, vanillin, asarone, myristic acid, thunbergol, cembrene, D-limonene, siloxanes, vinylguaicol, asarone, etc (Table 5). These are known to have potent antimicrobial [9,[39][40][41][42][43]45], anti-oxidant [41,43,44], anti-inflammatory [41,[43][44][45][46], analgesic [41,43,44], hypocholesterolemic [41,45], anti-hypertensive [47], anti-cancer [39,[43][44][45], biopesticide [48], nematicidal [48] effects of various intensities and are generally known to be safe for human use [41]. The pharmacobiological effects and therapeutic uses of these bioactives as summarised in Table 5 highlight the multifaceted beneficial role of Hawan.…”

Section: Discussionmentioning

confidence: 99%

“…Ltd., India), traditionally used as oblations in Hawan [8,14,15]. The composition of this plant mixture was as used in earlier work and reported [9]. Briefly it included -Acacia Arabica (Babool) bark;…”

Section: Hawan Ingredientsmentioning

confidence: 99%

“…Anti-(oxidant, tubercular, rheumatic, scabies, inflammatory), analgesic [9,30,31,33,40,41] 20% each n-Hexadecanoic (Palmitic) acid (a,b,c,e) Anti-oxidant [40,41,44], biopesticide [31,33,36], nematicide [33,48], hypocholesterolemic [31,33], lubricant [31] Oleic acid(a,b,c,e) Anti-(inflammatory, androgenic, cancer), hypocholesterolemic [ Table 5: GC-MS results of pyrolysed hawan ingredients and known pharmacobiological properties of identified compounds . a, compound identified on pyrolysis of W+G+S; b, compound identified on pyrolysis of ghee (G); c, compound identified on pyrolysis of wood (W); d, compound identified on pyrolysis of herbal mixture (S); e, compound identified on GC-MS of hawan smoke extract (emboldened to highlight significance); *Compound identified uniquely on pyrolysis of wood tivity.…”

Section: Thunbergol(d)mentioning

confidence: 99%

“…Recent studies have shown therapeutic potential of Hawan Medicinal Fumes in various non-communicable diseases like cancer, diabetes [6,7], as also in treatment of pulmonary tuberculosis [8,9], along with encouraging results of reduction in the bacteria and air pollutants post Hawan [10][11][12][13][14]. However, there was no study which had tested hawan thorough controlled (to overcome dilution and dispersion effects) and natural conditions for comprehensive simultaneous time-dependent effect on air quality, antibacterial activity, public health safety hindering its possible applicability in modern medicine.…”

Section: Introductionmentioning

confidence: 99%

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Quality analysis, Anti-bacterial activity and Chemical Characterization of Ethnobotanical (Hawan) Medicinal Fumes

Rastogi

Krishnanand

Panwar

et al. 2022

Interdis. J of Yagya Res.

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Background: In Vedic literature, the traditional ritual Hawan is said to purify the environment and cure diseases in an eco-friendly way. Previously studies have shown potential of herbal fume for anti-microbial activity as well as for therapeutic purposes along with reduction in the air pollutants post Hawan. However, there was no study which had tested and compared herbal fumes of hawan in control and natural environment for air-quality, anti-bacterial activity and for presence of bio-compounds present in time-dependent manner simultaneously. This work aims to evaluate air quality of hawan and simultaneously evaluate bio-compounds present in it to find its potential for integrative medicine. This work aims to investigate its efficacy in air purification and potential application in modern integrative medicine. Methods: Chambers for performing Hawan under natural and controlled experimental conditions were constructed. Hawan was performed for 30min in a chamber. Chamber air and Hawan herbal fumes were sampled before, during (30 min) and at end (24-72 hours) to find viable bacterial load of air, chemical bio-actives (phyto-constituents), and air quality markers (gases and pollutants) by standard techniques and Gas Chromatography Mass Spectroscopy (GC-MS) analysis along with analysis of pyrolysed hawan herbal fumes and Hawan ingredients. OneWay ANOVA with post hoc analysis by Tukey’s test was performed for comparison of air quality parameters during different experiments. Results: Transient sharp increase in CO, CO2, NOx levels (albeit within permissible limits) observed during Hawan was followed by decrease below baseline after 24hrs of Hawan in open-door natural conditions. Statistically significant persistent reduction (88-90\%) (p<0.0001) in the viable bacterial count of air compared to control was observed upto ≥72 hours after Hawan, when all pathogenic bacteria present in air were eliminated, leaving few \textit{Bacillus spp.} known to be beneficial for human health. GCMS analysis of Hawan medicinal fumes and pyrolysed Hawan ingredients revealed presence of an array of bioactive compounds known to have antimicrobial, anti-oxidant, air cleansing and nutritional activity and health benefits. Conclusion: Thus, through temporal and simultaneous analysis of Hawan air quality, its anti-microbial activity and phytochemical characterization, the study provided evidence-based-support to multifaceted potential of Hawan as described in ancient literature which can be put to immense use for therapeutic utility in modern time.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Hawan Ingredientsmentioning

confidence: 99%

Section: Thunbergol(d)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quality analysis, Anti-bacterial activity and Chemical Characterization of Ethnobotanical (Hawan) Medicinal Fumes

Rastogi

Krishnanand

Panwar

et al. 2022

Interdis. J of Yagya Res.

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Spectroscopic studies on the antioxidant and anti-tyrosinase activities of anthraquinone derivatives

Loganathan,

Akbar,

Ahmed

et al. 2023

Journal of King Saud University - Science

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Antimicrobial Effect of an Alcoholic Extract of an Ethnobotanical Mixture Against Clinical Isolates

Shrimali,

Kumar

2023

Interdis. J of Yagya Res.

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Infectious diseases, exacerbated by antibiotic-resistant bacteria, continue to pose a significant global burden. Traditional Medicine offers interesting possibilities for combating drug resistance, with various plants producing secondary metabolites that exhibit biological activities. This study aims to evaluate the antimicrobial effects of an alcoholic extract of an ethnobotanical mixture (Hawan Samagri) against clinical isolates. The mixture consists of six herbs commonly used in the Hawan ritual in India i.e. Giloy, Nagarmotha, Palash, Bakuchi, Bel, Kapoor kachari} and Chid. The study tested its extract against 11 clinical bacterial isolates using the microbroth dilution method. The results demonstrated that the alcoholic extract exhibited antimicrobial activity against all tested bacterial isolates, indicating potential broad-spectrum activity. The study also revealed varying levels of resistance among the isolates: Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa were most resistant, while Branhamella catarrhalis and Proteus vulgaris were relatively medium resistant, and the remaining five bacteria, i.e. Escherichia coli, Salmonella enterica, Coagulase-positive staphylococci, Citrobacter freundii and Coagulase-negative staphylococci, were relatively least resistant. Although the study provides valuable insights into the antimicrobial potential of the ethnobotanical mixture, further research is required to determine the specific concentrations, active compounds, and mechanisms of action along with their efficacy and optimal dosage. Nevertheless, these findings contribute to the use of indigenous resources for combating antimicrobial resistance and suggest the potential of incorporating such herbal mixtures into the daily practices of Hawan as a preventive measure.

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Anti tubercular minimum inhibitory concentration (MIC) and chemical characterization of ethnobotanical mixture used in the treatment of tuberculosis

Cited by 3 publications

References 8 publications

Quality analysis, Anti-bacterial activity and Chemical Characterization of Ethnobotanical (Hawan) Medicinal Fumes

Quality analysis, Anti-bacterial activity and Chemical Characterization of Ethnobotanical (Hawan) Medicinal Fumes

Spectroscopic studies on the antioxidant and anti-tyrosinase activities of anthraquinone derivatives

Antimicrobial Effect of an Alcoholic Extract of an Ethnobotanical Mixture Against Clinical Isolates

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