Anti-tumor activity of heat-killedLactobacillus plantarumBF-LP284 on Meth-A tumor cells in BALB/c mice

Abstract

Probiotics exert numerous effects on human well-being. Here, heat-killed Lactobacillus plantarum BF-LP284 (H-Lp) was isolated as a potent immuno-modulator among 15 strains of lactobacilli in terms of TNF-α induction ability in peritoneal macrophages. In vitro TNF-α and IFN-γ induction in Peyer's patch (PP) cells was higher when incubated with H-Lp than with live L. plantarum BF-LP284 (L-Lp). Suppression of syngeneic Meth-A tumors in a murine model by oral administration of H-Lp was also greater than that of L-Lp and of controls. H-Lp stimulated IFN-γ production in spleen cells, which displayed inhibited tumor growth in Winn assays when treated with H-Lp. Moreover, H-Lp increased the ratio of CD3(+ )cells among peripheral blood mononuclear cells in Meth-A tumor-bearing mice, suggesting an H-Lp-mediated anti-tumor mechanism whereby immune cells that are activated by H-Lp in PP and acquire anti-tumor activity in the spleen migrate to tumor sites through lymphocyte homing to inhibit tumor growth.

The immunomodulatory effects of probiotics on respiratory viral infections: A hint for COVID-19 treatment?

“…Since the manifestation of probiotics impacts on several diseases from non-viral to viral ones [ 12 , [37] , [38] , [39] , [40] , [41] ], several studies have surveyed the probiotic roles in immune responses of influenza-infected animal models. It has been fully demonstrated that upon infection with influenza, many cytokines such as IL-12 (one of the mediators of Th1 immune-response), interferon (IFN)-γ (representative of Th1 cytokine), IL-4 and IL-10 (Th2 cytokines), IL-1α, IL-1β, IL-6, and tumor necrosis factor (TNF)-α (pro-inflammatory cytokines), and IFN-α and IFN-β are produced in the respiratory tract [ 36 , [42] , [43] , [44] ].…”

“…Among several genera of bacteria (and yeasts) that identified and defined as probiotics, health benefits of Lactobacillus and Bifidobacterium on the host have been proved and are generally consumed as a part of fermented foods like those in dietary supplements [ 2 ]. There are some reports about probiotics potential in promoting health benefits by regulating allergic reactions [ [3] , [4] , [5] ], protecting the hosts against bacterial and viral infection [ 1 , [6] , [7] , [8] , [9] ], and even reducing the tumor growth in some cancer models [ [10] , [11] , [12] ]. The probiotics-conferred health benefits are attributable to their effects on the immune system.…”

Modulation of Anti-Tumour Immune Responses by Probiotic Bacteria

“…As a result, tumour growth was suppressed and microvessel density was lowered in an IL-17 dependent manner in treated animals. Finally, the oral administration of heat-inactivated Lactobacillus plantarum BF-LP284 in mice injected with Meth A tumour cells culminated in tumour volume reduction and the modulation of cytokine production and lymphocyte populations [119].…”

Exopolysaccharide from Lactobacillus plantarum LRCC5310 offers protection against rotavirus-induced diarrhea and regulates inflammatory response

“…Many potential health benefits of L. plantarum have been reported, such as effects on cholesterol, diarrhea, and irritable bowel syndrome (Barreto et al, 2014). After growing in glucose or sucrose, L. plantarum strains can produce EPS (Ismail and Exopolysaccharide from Lactobacillus plantarum LRCC5310 offers protection against rotavirus-induced diarrhea and regulates inflammatory response Nampoothiri, 2010), and the reported benefits include antioxidant (Zhang et al, 2013), antitumor (Shin et al, 2016), anti-inflammatory activity (Toshimitsu et al, 2017), as well as antiviral activity against herpes simplex virus (Matsusaki et al, 2016); however, no effects against rotavirus have been reported previously.…”