Anti-tumor effects of Abnormal Savda Munziq on the transplanted cervical cancer (U27) mouse model

Abstract

BackgroundAbnormal Savda Munziq (ASMq), a traditional uyghur medicine, has shown anti-tumour properties in vitro. it was showed that total flavonoids of ASMq could inhibit the proliferation and enhance the antioxidant ability of human cervix cancer HeLa cell. This study attempts to confirm these effects on the transplanted cervical cancer (U27) mouse model in vivo.MethodsForty eight Kunming mice were randomly divided in to six groups: normal control group (Control group), U27 tumor model group (Model group), cyclophosphamide administration group (CTX group),low-dose ASMq group (ASMq.L group), medium-dose ASMq group (ASMq.M group), and high-dose ASMq group (ASMq.H group). The five groups except normal control group transplanted with cervical cancer (U27) cells. We observed mice tumor inhibition rate and conducted the histopathological analysisUsing the western blot assay, the expression of TGF-β1 and TNF-α protein in transplanted cervical cancer U27 tumor tissue were detected.ResultsThe tumor inhibition rates of CTX group, ASMq.L group, ASMq.M group, and ASMq.H group were 72.21, 31.27, 60.53 and 51.94% respectively, has obvious antitumor effect. ASMq significantly promote the spleen tlymphocyte proliferation of transplanted cervical cancer U27 mice. Invasive growth and diffusion rate in tumor tissue were accelerate in the transplanted cervical cancer U27 model group. Tumor tissue necrosis of tumor cells are smaller in the medium, high dosage group. Compared with the U27 model group, the expression levels of TGF-β1 protein and TNF-α protein expression exhibited statistically significant decreased in the mice tumor tissues in the CTX administration group and the ASMq administration group.ConclusionsASMq has some antitumor effects on U27 model mice in vivo, The effects are achieved not only by improving the immune function of U27 model mice, but also by inhibiting the expression levels of TGF-β1 protein while promoting the expression levels of TNF-α protein.

Flavonoids: Anticancer Properties

“…ASMq groups with different dosages expressed decreased TGF-β1 protein and were increased in TNF-α proteins. Compared to CTX group, TGF-β1 protein expression of ASMq groups was decreased and protein level was increased in TNF-α [37].…”

ASMq protects against early burn wound progression in rats by alleviating oxidative stress and secondary mitochondria‑associated apoptosis via the Erk/p90RSK/Bad pathway

“…With advancements of science and technology, research into Uyghur traditional medicine has become more in-depth, with significant progress in drug extraction and component analysis (33)(34)(35). ASMq, a natural compound of traditional Uyghur Medicine, has been reported to exhibit anti-inflammatory, antioxidant and anti-apoptotic effects, and decreases oxidative damage by free radicals (10)(11)(12)(13)(14)(15)(16)(17). The production of ASMq is subject to a patent application (no.…”

“…Uygur medicine, one of the most important branches of Chinese traditional medicine, provides ways to manage emergency situations and disease ( 16 , 30 – 32 ). In the systemic theory of Uyghur medicine, the liver produces bodily fluids with different effects that can be divided into four types: Sapra, kan hiliti, phlegm and sawda ( 15 , 16 ). There is a complementary balance between these fluids, which serves a role in the maintenance of normal physiology and the occurrence and development of disease.…”

“…Early intervention before progressive changes of initial deep burn wounds can prevent further deepening and extension, decrease damage of the dermis and subcutaneous tissue and prevent the generation of secondary hypertrophic scars ( 36 ). Based on previously reported anti-inflammatory, anti-apoptotic and antioxidant effects of ASMq in the treatment of various diseases ( 4 , 15 , 23 , 37 ) and the underlying mechanism of early burn wound progression, it was hypothesized that ASMq may have a potential therapeutic effect on the progressive deepening of early burn wounds. Oxygen free radicals are considered to be important mediators of tissue damage progression following thermal injury ( 9 , 38 ).…”

“…Abnormal Savda Munziq (ASMq), a traditional herbal formula in Uyghur medicine, primarily consists of ten herbs, including Lavandula angustifolia, Foeniculum vulgare, Anchusa italica, Euphorbia humifusae, Melissa officinalis, Adiantum capillus-veneris, Glycyrrhiza uralensis, Cordia dichotoma, Ziziphus jujuba and Alhagi pseudoalhagi (10). Previous reports demonstrated that ASMq exerts antioxidative, anti-inflammatory and anti-apoptotic properties to inhibit the formation of hypertrophic scars (4) and treat diabetes, cardiovascular disease, pulmonary disorders and various types of cancer, such as cervical cancer and hepatic carcinoma (10)(11)(12)(13)(14)(15)(16)(17); to the best of our knowledge, however, few studies have investigated its potential effects on burn injury. In consideration of the critical role of reactive oxygen species (ROS)-induced ASMq protects against early burn wound progression in rats by alleviating oxidative stress and secondary mitochondria-associated apoptosis via the Erk/p90RSK/Bad pathway oxidative stress and subsequent mitochondria-associated apoptosis in the pathophysiological development of early burn wounds, it was hypothesized that ASMq may prevent early burn wound progression in rats by suppressing oxidative stress and apoptosis.…”