Anti-tumor properties of orally administered glucosamine and N-acetyl-d-glucosamine oligomers in a mouse model

Masuda, Sachie; Aso, Kazuo; Kurozumi, Seizi; Kiyose, Masatoshi; Osaki, Tomohiro; Tsuka, Takeshi; Itoh, Norihiko; Imagawa, Tomohiro; Minami, Saburo; Sato, Kimihiko; Okamoto, Yoshiharu

doi:10.1016/j.carbpol.2014.04.102

Cited by 48 publications

(22 citation statements)

References 20 publications

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“…Furthermore, chitin derivatives can enhance the immune response and act as an antibiotic/probiotic in pregnant pig 52 . Various biological activities, and especially anti-cancer and anti-inflammatory action of chitin oligosaccharide and chitosan oligosaccharide, have been well studied 9 , 53 – 55 . Distinct chitooligosaccharides from chitin-containing organisms may improve immune systems or act as probiotics providing benefits for animal health.…”

Section: Discussionmentioning

confidence: 99%

Protease resistance of porcine acidic mammalian chitinase under gastrointestinal conditions implies that chitin-containing organisms can be sustainable dietary resources

Tabata

Kashimura

Wakita

et al. 2017

Sci Rep

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Chitin, a polymer of N-acetyl-D-glucosamine (GlcNAc), is a major structural component in chitin-containing organism including crustaceans, insects and fungi. Mammals express two chitinases, chitotriosidase (Chit1) and acidic mammalian chitinase (AMCase). Here, we report that pig AMCase is stable in the presence of other digestive proteases and functions as chitinolytic enzyme under the gastrointestinal conditions. Quantification of chitinases expression in pig tissues using quantitative real-time PCR showed that Chit1 mRNA was highly expressed in eyes, whereas the AMCase mRNA was predominantly expressed in stomach at even higher levels than the housekeeping genes. AMCase purified from pig stomach has highest activity at pH of around 2–4 and remains active at up to pH 7.0. It was resistant to robust proteolytic activities of pepsin at pH 2.0 and trypsin and chymotrypsin at pH 7.6. AMCase degraded polymeric chitin substrates including mealworm shells to GlcNAc dimers. Furthermore, we visualized chitin digestion of fly wings by endogenous AMCase and pepsin in stomach extract. Thus, pig AMCase can function as a protease resistant chitin digestive enzyme at broad pH range present in stomach as well as in the intestine. These results indicate that chitin-containing organisms may be a sustainable feed ingredient in pig diet.

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Section: Discussionmentioning

confidence: 99%

Protease resistance of porcine acidic mammalian chitinase under gastrointestinal conditions implies that chitin-containing organisms can be sustainable dietary resources

Tabata

Kashimura

Wakita

et al. 2017

Sci Rep

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“…or i.p., and there is not much reported on the beneficial effects of NACOS and COS following oral administration. We recently assessed the anticancer properties of orally administered NACOS and COS in a mouse model of colon cancer using the cell line, colon-26 [ 22 ]. We observed that in animals receiving either COS (2% and 4%) or NACOS (2% and 4%), tumor volumes were significantly lower than those in control group ( p < 0.05) ( Figure 1 ).…”

Section: Anti-cancer Effects Of Nacos and Cos Following Oral Adminmentioning

confidence: 99%

Anticancer and Anti-Inflammatory Properties of Chitin and Chitosan Oligosaccharides

Aso

Osaki

Minami

et al. 2015

JFB

Self Cite

263

119

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Previous reports indicate that N-acetyl-d-glucosamine oligomers (chitin oligosaccharide; NACOS) and d-glucosamine oligomers (chitosan oligosaccharide; COS) have various biological activities, especially against cancer and inflammation. In this review, we have summarized the findings of previous investigations that have focused on anticancer or anti-inflammatory properties of NACOS and COS. Moreover, we have introduced recent evaluation of NACOS and COS as functional foods against cancer and inflammatory disease.

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“…Derived from the hydrolysis of chitosan, the chitooligosaccharides have several biofunctional properties, including antiinflammatory , prebiotic , antimicrobial , antitumor , and immunopotentiating effects . Therefore, the nutraceutical and medical industries have given special attention to the production of oligosaccharides from chitosan .…”

Section: Introductionmentioning

confidence: 99%

Recovery and purification of chitosanase produced by Bacillus cereus using expanded bed adsorption and central composite design

Araújo

Pimentel

Silva

et al. 2016

J of Separation Science

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This study presents a system for expanded bed adsorption for the purification of chitosanase from broth extract in a single step. A chitosanase-producing strain was isolated and identified as Bacillus cereus C-01 and used to produce chitosanases. The expanded bed adsorption conditions for chitosanase purification were optimized statistically using STREAMLINE(TM) DEAE and a homemade column (2.6 × 30.0 cm). Dependent variables were defined by the quality criteria purification factor (P) and enzyme yield to optimize the chromatographic process. Statistical analyses showed that the optimum conditions for the maximum P were 150 cm/h load flow velocity, 6.0 cm settled bed height, and 7.36 cm distributor height. Distributor height had a strong influence on the process, considerably affecting both the P and enzyme yield. Optimizing the purification variables resulted in an approximately 3.66-fold increase in the P compared with the value under nonoptimized conditions. This system is promising for the recovery of chitosanase from B. cereus C-01 and is economically viable because it promotes the reduction steps.

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Anti-tumor properties of orally administered glucosamine and N-acetyl-d-glucosamine oligomers in a mouse model

Cited by 48 publications

References 20 publications

Protease resistance of porcine acidic mammalian chitinase under gastrointestinal conditions implies that chitin-containing organisms can be sustainable dietary resources

Protease resistance of porcine acidic mammalian chitinase under gastrointestinal conditions implies that chitin-containing organisms can be sustainable dietary resources

Anticancer and Anti-Inflammatory Properties of Chitin and Chitosan Oligosaccharides

Recovery and purification of chitosanase produced by Bacillus cereus using expanded bed adsorption and central composite design

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