Anti-Vibrio Activity of Parkia javanica: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation on Four Vibrio cholarae Strains

Saha, Susmita; Mullick, Jhinuk Basu; Choudhury, Parichita Ray; Saha, Partha; Chakraborty, Debaprasad; Sil, Samir Kumar

doi:10.20546/ijcmas.2016.508.058

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…[36,37] Several studies have reported the antibacterial, anti-inflammatory, and wound healing activities of P. javanica bark. [38][39][40][41] The methanolic extract of P. javanica bark (MEPJB) has also been reported to inhibit various blood cell malignancies. [42] However, there is no scientific study on the anti-colon cancer activity of P. javanica bark.…”

Section: Discussionmentioning

confidence: 99%

Anti-colon Cancer Activity of Parkia javanica (Lamk.) Merr. Bark Extract: An In-vitro Study

Saha¹,

Saha²,

Sil³

2021

Int. J. Pharm. Sci. Drug Res.

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The Methanolic extract of Parkia javanica bark (MEPJB) was examined in vitro to see whether it has any cytotoxic or chemotherapeutic potential against the colon cancer cell lines HCT116 and SW480. Cell viability or proliferation assay and migration assay were performed, which showed significant results. The bark extract exhibited significant cytotoxic potential against both the colon cancer cell lines, and it also inhibited cell migration in a dose-dependent and time-dependent experiment. The IC₅₀ values of MEPJB on the HCT116 cell line for 24 hours, 48 hours, and 72 hours were 83.33 μg/mL, 37.5 μg/mL, and 34.37 μg/mL, respectively, whereas on the SW480 cell line, the IC₅₀ values were 87.5 μg/mL, 40 μg/mL and 31.25 μg/mL for 24 hours, 48 hours, and 72 hours respectively. However, IC₅₀ doses of MEPJB were inactive against the normal, healthy human fibroblast cell line. Cytotoxicity screening of 5-fluorouracil (5-FU), a commonly used drug in colon cancer treatment, was also done on both the colon cancer cell lines HCT116 and SW480, which showed much higher IC₅₀ than MEPJB after 24 hours, 48 hours, and 72 hours of incubation. MEPJB changed the cell morphology and induced apoptosis in colon cancer cell lines, confirmed by a morphological study of treated and untreated colon cancer cell lines and the acridine orange/ethidium bromide (AO/EB) dual staining procedure, respectively. After 48 hours of incubation with respective IC₅₀ concentrations of MEPJB, the treated colon cancer cells showed early and late apoptotic features. So this current research work indicates that the methanolic extract of P. javanica bark has chemotherapeutic potential against colon cancer cells and promotes apoptosis in cancer cells, which results in cell death.

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Section: Discussionmentioning

confidence: 99%

Anti-colon Cancer Activity of Parkia javanica (Lamk.) Merr. Bark Extract: An In-vitro Study

Saha¹,

Saha²,

Sil³

2021

Int. J. Pharm. Sci. Drug Res.

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“…This plant is widely used by tribal population of northeast region of India 11,12,13 . In our previous study, it was found that, crude methanol extract of Parkia javanica possess antibacterial activity against both standard ATCC strains and MDR strains and ROS induced DNA damage may be possible mediator of antibacterial activity of this plant 14,15,16 . Therefore, this work has been undertaken to explore the active fraction of this study plant having antibacterial properties against gram negative multi drug resistant bacterial strains with possible mode of action.…”

Section: Introductionmentioning

confidence: 96%

Chloroform Fraction of Parkia Javanica Bark Possesses Antibacterial Activity Against Multidrug Resistant Gram Negative Bacteria Predominantly Found in Skin Wound

Saha

Karmakar

Sil

2018

J. Drug Delivery Ther.

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Aim: To evaluate the antibacterial activity of Parkia javanica against gram negative MDR bacterial strains which are predominantly found in skin wound. Methods: The 5 different solvent fractions of Parkia javanica were screened for antibacterial activity against gram negative multi drug resistant bacterial strains namely Enterobacter aerugenes, Pseudomonas aeruginosa and Klebsiella pneumonia by serial dilution technique. Growth kinetics study was performed and percentage of ROS production was measured by NBT reduction assay. Results: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were obtained with a range of IC100 0.08-0.31 mg/ml in case of MDR bacterial strains. The lag phase of all extract treated bacteria is extended compared to untreated cells. The normalized % of ROS is increased in presence of Parkia javanica extract. Conclusions: This study suggests that, chloroform fraction of Parkia javanica possesses promising antimicrobial substances which are having activity against MDR bacterial strains and ROS induced bacterial cell damage could be the possible mediator of its antimicrobial activity. Keywords: Parkia javanica, antibacterial activity, MDR bacterial strains, growth curve, ROS.

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Identiﬁcation of 2,4-Di-tert-butylphenol (2,4-DTBP) as the Major Contributor of Anti-colon cancer Activity of Active Chromatographic Fraction of Parkia javanica (Lamk.) Merr. Bark Extract

Saha

Sharma²,

Dash

et al. 2023

Biomed. Pharmacol. J.

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Parkia javanica (Lamk.) Merr. is an ethnomedicinal leguminous plant species from northeastern India with a long history of medicinal use among various tribes of this region to treat cholera, dysentery, stomach aches, diarrhea and food poisoning, having antibacterial, wound-healing, anticancer and anti-inflammatory properties. Therefore, in this current study, the methanolic bark extract was carried out and fractionated by using flash chromatography, examined the cytotoxicity of the respective fractions on colon cancer cell lines, and evaluated the major phytochemical compounds present in the fractions using Gas Chromatography-Mass Spectrometry (GC-MS) chemical profiling. Chemical profiling of the fractions by GC-MS revealed in fraction-1 and -2, 2,4-Di-tert-butylphenol was the major compound (50.740% in fraction-1, 21.277% in fraction-2, and 7.859% in fraction-3) having reported anticancer activity. The gradation of the presence of this compound in the fractions was corroborated by the gradation of anti-colon cancer activity of the respective fractions on both the colon carcinoma cell lines. However, the presence of D-Allose in a substantial amount (20.870%) in only fraction-3 could not increase the anticancer activity of fraction-3 over the other two fractions. An in vitro cytotoxic assay guided evaluation of three flash chromatographic fractions (fraction-1, -2, and -3) of methanolic extract of Parkia javanica bark showed significant anticancer properties on two human colon carcinoma cell lines (HCT116 and SW480). The order of efficacy of the fractions was fraction-1> fraction-2 > fraction-3. In a time and dose-dependent experiment, fraction-1, being the most active one, showed an IC50 value of 16.25 µgml-1 (24 hrs), 9.94 µgml-1 (48 hrs), and 9.38 µgml-1 (72 hrs) on HCT116 and 35 µgml-1 (24 hrs), 20.14 µgml-1 (48 hrs), and 19.71 µgml-1 (72 hrs) on the SW480 cell line. Parkia javanica bark extract is bestowed with the potential of anti-colon cancer property and upon chemical profiling of different chromatographic fractions of the extract, 2,4-Di-tert-butylphenol has been identified as the primary anticancer component of the extract.

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Anti-Vibrio Activity of Parkia javanica: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation on Four Vibrio cholarae Strains

Cited by 3 publications

References 7 publications

Anti-colon Cancer Activity of Parkia javanica (Lamk.) Merr. Bark Extract: An In-vitro Study

Anti-colon Cancer Activity of Parkia javanica (Lamk.) Merr. Bark Extract: An In-vitro Study

Chloroform Fraction of Parkia Javanica Bark Possesses Antibacterial Activity Against Multidrug Resistant Gram Negative Bacteria Predominantly Found in Skin Wound

Identiﬁcation of 2,4-Di-tert-butylphenol (2,4-DTBP) as the Major Contributor of Anti-colon cancer Activity of Active Chromatographic Fraction of Parkia javanica (Lamk.) Merr. Bark Extract

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