Anti-Vibrio parahaemolyticus compounds from Streptomyces parvus based on Pan-genome and subtractive proteomics

Liu, Wenbin; Ou, Peiyu; Tian, Fang; Liao, James C.; Ma, Yue; Wang, Jie; Jin, Xiaobao

doi:10.3389/fmicb.2023.1218176

Cited by 3 publications

(3 citation statements)

References 58 publications

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“…The Gram-negative bacterium V. parahemolyticus is a leading sea-food-borne pathogen worldwide, and common clinical symptoms include headache, nausea, vomiting, and diarrhea. Severe infections caused by V. parahemolyticus can develop into sepsis or even death [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Ingredients and Modes of the Methanol-Phase Extract from the Fruit of Amomum villosum Lour.

Zhang,

Cao,

Zhao

et al. 2024

Plants

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Epidemics of infectious diseases threaten human health and society stability. Pharmacophagous plants are rich in bioactive compounds that constitute a safe drug library for antimicrobial agents. In this study, we have deciphered for the first time antibacterial ingredients and modes of the methanol-phase extract (MPE) from the fruit of Amomum villosum Lour. The results have revealed that the antibacterial rate of the MPE was 63.64%, targeting 22 species of common pathogenic bacteria. The MPE was further purified by high performance liquid chromatography (Prep-HPLC), and three different constituents (Fractions 1–3) were obtained. Of these, the Fraction 2 treatment significantly increased the cell membrane fluidity and permeability, reduced the cell surface hydrophobicity, and damaged the integrity of the cell structure, leading to the leakage of cellular macromolecules of Gram-positive and Gram-negative pathogens (p < 0.05). Eighty-nine compounds in Fraction 2 were identified by ultra HPLC-mass spectrometry (UHPLC-MS) analysis, among which 4-hydroxyphenylacetylglutamic acid accounted for the highest 30.89%, followed by lubiprostone (11.86%), miltirone (10.68%), and oleic acid (10.58%). Comparative transcriptomics analysis revealed significantly altered metabolic pathways in the representative pathogens treated by Fraction 2 (p < 0.05), indicating multiple antibacterial modes. Overall, this study first demonstrates the antibacterial activity of the MPE from the fruit of A. villosum Lour., and should be useful for its application in the medicinal and food preservative industries against common pathogens.

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Section: Resultsmentioning

confidence: 99%

Antibacterial Ingredients and Modes of the Methanol-Phase Extract from the Fruit of Amomum villosum Lour.

Zhang,

Cao,

Zhao

et al. 2024

Plants

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“…is a group of filamentous Gram-positive mycelial Actinobacteria that are well-established candidates with antimicrobial, antioxidant, anticancer and immunosuppressant effects [157][158][159][160][161][162][163][164] . Their ability to produce a myriad of compounds piques the interest of researchers to identify valuable compounds that could hinder the growth of Vibrios [143,[165][166][167] . In the cross-streak assay, Streptomyces MUM 178J demonstrated antagonistic effects against MDR V. parahaemolyticus, RP0132.…”

Section: Discussionmentioning

confidence: 99%

“…An anti-V. parahaemolyticus compound, Actinomycin D was discovered in Streptomyces parvus during molecular docking studies done by Liu et al [165] . Actinomycin D was proposed to bind strongly to the flagellar motor switch protein FliN of V. parahaemolyticus, thereby impairing its motility and preventing biofilm formation [165] . You et al [166] isolated several Streptomyces sp.…”

Section: Discussionmentioning

confidence: 99%

Detection of multidrug resistant Vibrio parahaemolyticus and anti-Vibrio Streptomyces sp. MUM 178J

Loo,

Tan,

Law

et al. 2023

Prog Micobes Mol Biol

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The growing global population has increased the demand for seafood, making the aquaculture industry a vital food source. However, climate change has negatively impacted the industry as natural inhabitants of aquatic environments such as Vibrio parahaemolyticus are thriving in the warming waters. These foodborne pathogens can cause disease in marine animals, resulting in lowered production rates and substantial economic losses. V. parahaemolyticus is transmitted from these marine animals in aquaculture to humans via raw or undercooked seafood, resulting in gastroenteritis outbreaks. In addition, the extensive use of antibiotics in aquaculture to treat infections within marine animals has led to the development of multidrug-resistant (MDR) V. parahaemolyticus, which threatens the effectiveness of antimicrobial treatments. Thus, it is vital to explore the alternatives of antibiotics in aquaculture to manage the spread of antibiotic resistance. This study aimed to investigate the prevalence of MDR V. parahaemolyticus to provide insight into the current antibiotic resistance patterns of this pathogen and to determine potential anti-Vibrio properties of Streptomyces MUM 178J which was previously isolated from the soils of a mangrove forest in Sarawak, Malaysia. Colony morphology and toxR-assay indicated that all samples tested positive for V. parahaemolyticus. Further identification with genomic analyses confirmed that 64% (64/100) of the isolates were V. parahaemolyticus. The multiple antibiotic resistance index (MAR) of the isolates ranged between 0.07 and 0.57, with 81% (52/64) of the isolates resistant to more than one antibiotic. The isolates were most resistant to ampicillin (98.4%), followed by ceftazidime (53.1%). In contrast, the isolates were highly susceptible to nalidixic acid (98.4%), tetracycline (93.8%), and sulfamethoxazole/trimethoprim (90.6%). They also demonstrated susceptibilities of 89.1% to levofloxacin, oxytetracycline, and gentamicin. Streptomyces sp. MUM 178J exhibited antagonistic effects on select V. parahaemolyticus strain, RP0132, with a minimum inhibitory concentration of 12.5 mg/mL and a minimum bactericidal concentration of 50 mg/mL.

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Subtractive genomics and comparative metabolic pathways profiling revealed novel drug targets in Ureaplasma urealyticum

Chen,

Zhang,

Zeng

et al. 2024

Front. Microbiol.

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IntroductionUreaplasma urealyticum is a commensal organism found in the human lower genitourinary tract, which can cause urogenital infections and complications in susceptible individuals. The emergence of antibiotic resistance, coupled with the absence of vaccines, underscores the necessity for new drug targets to effectively treat U. urealyticum infections.MethodsWe employed a subtractive genomics approach combined with comparative metabolic pathway analysis to identify novel drug targets against U. urealyticum infection. The complete proteomes of 13 Ureaplasma strains were analyzed using various subtractive genomics methods to systematically identify unique proteins. Subsequently, the shortlisted proteins were selected for further structure-based studies.ResultsOur subtractive genomics analysis successfully narrowed down the proteomes of the 13 Ureaplasma strains to two target proteins, B5ZC96 and B5ZAH8. After further in-depth analyses, the results suggested that these two proteins may serve as novel therapeutic targets against U. urealyticum infection.DiscussionThe identification of B5ZC96 and B5ZAH8 as novel drug targets marked a significant advancement toward developing new therapeutic strategies against U. urealyticum infections. These proteins could serve as foundational elements for the development of lead drug candidates aimed at inhibiting their function, thereby mitigating the risk of drug-resistant infections. The potential to target these proteins without inducing side effects, owing to their specificity to U. urealyticum, positions them as promising candidates for further research and development. This study establishes a framework for targeted therapy against U. urealyticum, which could be particularly beneficial in the context of escalating antibiotic resistance.

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Anti-Vibrio parahaemolyticus compounds from Streptomyces parvus based on Pan-genome and subtractive proteomics

Cited by 3 publications

References 58 publications

Antibacterial Ingredients and Modes of the Methanol-Phase Extract from the Fruit of Amomum villosum Lour.

Antibacterial Ingredients and Modes of the Methanol-Phase Extract from the Fruit of Amomum villosum Lour.

Detection of multidrug resistant Vibrio parahaemolyticus and anti-Vibrio Streptomyces sp. MUM 178J

Subtractive genomics and comparative metabolic pathways profiling revealed novel drug targets in Ureaplasma urealyticum

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