Anti-vibrio potentials of acetone and aqueous leaf extracts of &lt;i&gt;Ocimum gratissimum&lt;/i&gt; (Linn)

Igbinosa, Etinosa O.; Idemudia, Omoruyi G.

doi:10.4314/tjpr.v15i4.11

Cited by 9 publications

(8 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous report has depicted the anti-Vibrio activity of the plant Ocimum gratissimum Linn. (23). Present study has yielded a positive result for antibacterial activity of RDRC extract against V. vulnificus, as the zone of inhibition was found to be about 10 mm.…”

Section: Resultsmentioning

confidence: 49%

Anti-vibrio effects of the precious Tibetan pill, Rinchen Drangjor Rilnag Chenmo (RDRC)

et al. 2021

View full text Add to dashboard Cite

Tibetan precious pills are an integral part of TTM (Traditional Tibetan Medicine). Among them, Rinchen Drangjor Rilnag Chenmo (RDRC) has been named “King of Precious Pills” due to its efficacy in treating a multitude of human disorders. RDRC has a complex formulation with about 140 ingredients, mostly from medicinal plants and a few precious stones and metals. Not many studies have been done on the experimental validation of antimicrobial properties of this important pill. The current study investigated the antimicrobial activity of the extracts of RDRC. Both aqueous and chloroform extracts were evaluated for their antibacterial potential against a total of seven different bacterial species, which are pathogenic, including three species of Vibrio, viz. V. vulnificus, V. parahaemolyticus and V. harveyi using the well-diffusion method and also by assessing MIC and MBC values. Its antifungal potential was also studied against two fungal strains Aspergillus niger and Talaromyces islandicus. It was found that the chloroform extract of RDRC exerted a positive antibacterial effect on all the Vibrio species tested, and the least MIC of 3.33 mg/ml was observed for V. parahaemolyticus. This is the first study of its kind on the anti-Vibrio effect of the Tibetan precious pill, Rinchen Drangjor Rilnag Chenmo.

show abstract

Section: Resultsmentioning

confidence: 49%

Anti-vibrio effects of the precious Tibetan pill, Rinchen Drangjor Rilnag Chenmo (RDRC)

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Five millilitre (5 mL) of spore suspension from the Streptomyces isolate was introduced into 100 mL of yeast malt extract broth in 250 mL Erlenmeyer flask and incubated in a shaker sustained for 7 d at 150 rpm as previously described by Singh et al [16] with modification. The antimicrobial activity was elucidated against the test bacteria via agar well diffusion method [29] . Likewise, the influence of pH on the antimicrobial agent produced was studied at dissimilar pH (5, 6, 7, 8 and 9) using yeast malt extract broth incubated for 7 d at 28°C.…”

Section: Effect Of Temperature Ph and Incubation Period On Growth Anmentioning

confidence: 99%

“…Multidrug resistance has continuously been a challenge that has led to researches on new improved drugs from various sources [2] . Antimicrobial resistance has posed a major challenge to medicine; it has cost the pharmaceutical industries a lot of finance in research and development [29] . Great contributions of new drugs from natural sources have come from countries like Japan, United States, India, China and Germany; with little report from Africa.…”

Section: Preliminary Screening Of Tentative Isolates Of Streptomyces mentioning

confidence: 99%

“…Staphylococcus aureus ATCC 12598 + n/a n/a n/a n/a n/a Bacillus cereus ATCC 14579 + n/a n/a n/a n/a n/a Escherichia coli ATCC 29214 -n/a n/a n/a n/a n/a Proteus vulgaris ATCC 15315 -2.5 >10 n/a n/a n/a Listeria monocytogenes ATCC 7644 + n/a n/a n/a n/a n/a Listeria monocytogenes Scott A + n/a n/a n/a n/a n/a Salmonella enterica serovar Typhimurium ATCC 14028 -n/a n/a n/a n/a n/a Shigella flexneri ATCC 120222 -n/a n/a >10 n/a >10 Legend: n/a -Not Applicable Time-kill kinetics has been applied to elucidate activities of diverse antibacterial metabolites. The technique is frequently applied as the basis for in vitro research into pharmacodynamics of drug activities [29] . It yields expressive (qualitative) data on the pharmacodynamics of antibacterial peptides [10] .…”

Section: Minimum Inhibitory Concentration (Mic) Of the Streptomyces Smentioning

confidence: 99%

See 1 more Smart Citation

Isolation and Characterization of Antibacterial Metabolites Produced by Streptomyces species from Escravos River, Delta State, Nigeria

Igbinosa¹,

Igelige²,

Igbinosa³

et al. 2017

TJNPR

View full text Add to dashboard Cite

Introduction Actinomycetes have been described as the basis which forms the significant number of novel antibiotic drugs, with its use in recycling of organic matter and in the treatment of ailment [1-4]. Actinomycetes comprise an extensive amount of the populace of lakes, soil, marine water, fresh water and river muds [5]. Naturally, actinomycetes have been recovered from lithosphere sources. However, the principal documentation of mycelium producing actinomycetes obtained from marine sediments surfaced decades ago. Marine sediments have been described as pioneer origin for the recovery of novel actinomycetes producing new yields and are acknowledged as potential sources of new anticancer and antibiotic agents [6]. Secondary by-products with anticellular activity formed by actinomycetes harbours a broad range of biological activities [7-8]. Streptomyces are regarded as a group of Gram positive filamentous bacteria that are found in diverse natural environmental sources [9-11]. The genus Streptomyces alone accounts for a remarkable number of antibiotics. It possesses a massive biosynthetic significance that remains unrivalled without a meaningful competitor among other microbial populations [12]. Streptomyces are especially prolific, most economically valuable

show abstract

“…The anti-microbial activity of the OG extract has been tested in vitro against various human pathogens like Klebsiella pneumoniae, Staphylococcus aureus, Escherichia, Vibro spp, Enterobacter spp, Enterococcus faecalis, etc. The antifungal activity of the essential oil of OG extracts has been noted in vitro [19,21,22], and OG has been used as a mosquito appellant [23]. A recent animal study suggested the importance or protective effect of OG extracts in the prevention of blood pressure irregularities against cobalt-chloride toxicity [24] as well as attenuating cardiac abnormalities associated with liver brosis by downregulating the interleukin-6 signaling pathway [25] or by inducing antioxidant effects [26].…”

Section: Introductionmentioning

confidence: 99%

Polyphenol-rich extract of Ocimum gratissimum leaves Prevented Toxic Effects of Cyclophosphamide on the kidney Function of Wistar rats

Alabi

Akomolafe

et al. 2021

Preprint

View full text Add to dashboard Cite

Background Cyclophosphamide (CP) is one of the potent and low cost chemotherapy used in clinical setting against a variety of tumors. However, its association with nephrotoxicity limits its therapeutic use. Ocimum gratissimum leaf is a natural plant with numerous pharmacological and therapeutic efficacies, such as antioxidant, anti-inflammation, and anti-apoptotic properties.Methods The present study was designed to evaluate the protective effect of Ocimum gratissimum (OG) against CP-induced kidney dysfunction in rats. Rats were pre-treated with 400 mg/kg b.w. of polyphenol-rich Ocimum gratissimum leaves (PREOG) for 4 days and then 50 mg/kg b.w. of CP was co-administered from day 5 to day 7 along with PREOG. Markers of renal function and oxidative stress, food and water intake, electrolytes, aldosterone, leukocytes infiltration, inflammation and histopathological alteration were evaluated.Results Obvious renal inflammation and kidney injuries were observed in CP treated groups. PREOG administration prevented oxidative stress, kidney injuries, attenuated inflammation, increased aldosterone production and reduced sodium and water loss in rats. PREOG also decreased the plasma concentrations of Interleukin-(IL)-6, C-reactive protein and activity of myeloperoxidase and malondialdehyde in CP treated rats.Conclusion OG prevented kidney injury and enhanced renal function via inhibiting inflammation and oxidant-induced CP toxicity. The efficacy of OG is related to the presence of various phytochemicals in the plant.

show abstract

Anti-vibrio potentials of acetone and aqueous leaf extracts of <i>Ocimum gratissimum</i> (Linn)

Cited by 9 publications

References 20 publications

Anti-vibrio effects of the precious Tibetan pill, Rinchen Drangjor Rilnag Chenmo (RDRC)

Anti-vibrio effects of the precious Tibetan pill, Rinchen Drangjor Rilnag Chenmo (RDRC)

Isolation and Characterization of Antibacterial Metabolites Produced by Streptomyces species from Escravos River, Delta State, Nigeria

Polyphenol-rich extract of Ocimum gratissimum leaves Prevented Toxic Effects of Cyclophosphamide on the kidney Function of Wistar rats

Contact Info

Product

Resources

About