Anti-VP1 and anti-VP2 antibodies detected by immunofluorescence assays in patients with acute human parvovirus B19 infection

Pereira, Renata Freire Alves; Paula, Walquiria N.S. de; Cubel, Rita de Cassia N; Nascimento, Jussara Pereira do

doi:10.1590/s0074-02762001000400011

Cited by 7 publications

(5 citation statements)

References 33 publications

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“…The diagnosis was auto-immune hemolytic anemia due to HIV virus, and he was started on prednisone, 40mg PO qd. On January 19th a peripheral blood dot blot 8 and IgG antibody test 12 were both positive for parvovirus B19. A new bone marrow biopsy confirmed previous findings.…”

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confidence: 99%

Clinical presentation of parvovirus B19 infection in HIV-infected patients with and without AIDS

Setúbal

Jorge-Pereira

Sant'Anna

et al. 2003

Rev. Soc. Bras. Med. Trop.

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confidence: 99%

Clinical presentation of parvovirus B19 infection in HIV-infected patients with and without AIDS

Setúbal

Jorge-Pereira

Sant'Anna

et al. 2003

Rev. Soc. Bras. Med. Trop.

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“…Immunohistochemistry -Smears done with insect cells (Spodoptera frugiperda, Sf9) infected with recombinant baculovirus expressing either VP1 or VP2 erythrovirus B19 capsid proteins (Pereira et al 2001) were used to standardize the primary antibody dilution, made with Dako B0091 polyclonal anti-VP2 rabbit antibody. The best results were found with the 1:50 dilution.…”

Section: He -mentioning

confidence: 99%

“…They are frequent in bone marrow and fetal tissues infected by erythrovirus B19 (Ozawa et al 1986, Brown 1997, Koduri 1998, Krause et al 1992, Liu et al 1997. Giant pronormoblasts, the counterpart of "lantern cells" in bone marrow Wright-Giemsastained smears (Koduri 1998, Krause et al 1992 were not studied, as these smears were unavailable.Immunohistochemistry -Smears done with insect cells (Spodoptera frugiperda, Sf9) infected with recombinant baculovirus expressing either VP1 or VP2 erythrovirus B19 capsid proteins (Pereira et al 2001) were used to standardize the primary antibody dilution, made with Dako B0091 polyclonal anti-VP2 rabbit antibody. The best results were found with the 1:50 dilution.…”

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confidence: 99%

Erythrovirus B19 infection in acquired immunodeficiency syndrome: screening by histopathology, immunohistochemistry, and in situ hybridization

Setúbal

Oliveira

Pires

et al. 2006

Mem. Inst. Oswaldo Cruz

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Erythrovirus B19 infects erythrocytic progenitors, transiently interrupting erythropoiesis. In AIDS patients it causes chronic anemia amenable to treatment. We looked for evidences of B19 infection in stored bone marrow material from patients with acquired immunodeficiency syndrome. Histological sections were made from stored paraffin blocks from 33 autopsies (39 blocks) and 35 biopsies (45 blocks, 30 patients) performed from 1988 to 2002. They were examined after hematoxylin-eosin (HE) staining, immunohistochemical (IHC), and in situ hybridization. HE revealed intra-nuclear inclusion bodies ("lantern cells") suggesting B19 infection in 19 sections corresponding to 19 of 63 patients examined with this test. Seven of 78 sections subjected to immunohistochemistry were positive, corresponding to 7 of 58 patients examined with this test. Fourteen sections corresponding to 13 of the 20 HE and/or IHC positive patients were subjected to in situ hybridization, with six positives results. Among the 13 patients subjected to the three techniques, only one gave unequivocal positive results in all and was considered a true positive. The frequency of B19 infection (1/63 patients) in the material examined can be deemed low.Key words: parvovirus B19-anemia -chronic-immunodeficiency Erythrovirus B19 infects human erythrocytic progenitor cells, leading to a transient interruption of erythropoiesis. In the immunocompetent host this viral infection is cleared within two weeks as a result of the appearance of serum specific antibodies, and the hematological manifestations are restricted to a slight and asymptomatic decline in the hemoglobin levels. When the antibodies appear there may be symptoms of immune complex disease like arthralgias and exanthema. This is what normally takes place during the common childhood exanthematic disease erythema infectiosum. The course can be quite different in certain kinds of host, like those with hereditary hemolytic anemias (e.g., sickle-cell anemia), in whom an acceptable hematocrit is maintained only through an intense and uninterrupted bone marrow activity. In this context erythrovirus B19 infection usually leads to the rapid onset of a life threatening acute anemia, the so called "transient aplastic crisis". The patient is transfusion-dependent until the appearance of specific antibodies spontaneously clears the infection. A similar situation can be found in the fetus, where normal erythron volume expansion also demands a high activity of the hematopoietic fetal tissues. The sudden interruption of hematopoiesis leads to fetal heart failure and anasarca (hydrops fetalis), which may result in fetal death. In immunocompromised individuals (e.g., HIV-infected patients) erythrovirus infection is not readily cleared and its long persistence leads to chronic anemia. In these patients, intravenous standard immunoglobulin infusions usually end viremia and restore hematopoiesis (de Mayolo & Temple 1990, Mitchell et al. 1990, Gottlieb & Deutsch 1992, Noronha et al. 2005.Erythrovirus B19 was discovered by...

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“…The baculovirus expression system provides a method for the production of large quantities of biologically active and antigenic eukaryotic proteins for enzyme immunoassays and for immunoblot analysis and immunofluorescence assays (IFAs) (9,15,18,19,29). In the present study, we expressed the F protein of hMPV in Trichoplusia ni (Tn5) insect cells by a baculovirus system and demonstrated the utility of the recombinant F protein in an IFA.…”

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confidence: 95%

Immunofluorescence Assay for Detection of Human Metapneumovirus-Specific Antibodies by Use of Baculovirus-Expressed Fusion Protein

Ishiguro

Ebihara

Endo

et al. 2005

Clin Vaccine Immunol

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Anti-VP1 and anti-VP2 antibodies detected by immunofluorescence assays in patients with acute human parvovirus B19 infection

Abstract: Acute human parvovirus B19 infection is followed by an antibody response to the structural proteins of the viral capsid (VP1 and VP2

Cited by 7 publications

References 33 publications

Clinical presentation of parvovirus B19 infection in HIV-infected patients with and without AIDS

Clinical presentation of parvovirus B19 infection in HIV-infected patients with and without AIDS

Erythrovirus B19 infection in acquired immunodeficiency syndrome: screening by histopathology, immunohistochemistry, and in situ hybridization

Immunofluorescence Assay for Detection of Human Metapneumovirus-Specific Antibodies by Use of Baculovirus-Expressed Fusion Protein

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