Antiaging Activity of Peptide Identified from Fermented Trapa Japonica Fruit Extract in Human Dermal Fibroblasts

Abstract

We have previously shown that Trapa japonica fruit extract (TJE) as well as its fermented extract (FTJ) can be potentially used to treat alopecia. In the current study, a newly synthesized peptide (PEP) was detected in an active compound isolated from FTJ. Several biological assays were conducted to verify the antiaging effects of TJE, FTJ, and PEP on the skin. We examined the effects of TJE, FTJ, and PEP on cell viability, collagen synthesis, and inhibition of mRNA expression of matrix metalloproteinases (MMPs), induced by tumor necrosis factor alpha (TNF-α), in human dermal fibroblasts (HDFs). In addition, a wound-healing assay of the human keratinocyte cell line (HaCaT) and a clinical study of antiaging activity were conducted. The findings confirmed that PEP exerted an effect on cell proliferation in a dose-dependent manner. Treatment with TJE, FTJ, and PEP increased collagen synthesis but inhibited TNF-α-induced mRNA expression of MMPs. Compared with TJE and FTJ, PEP promoted a significant level of wound recovery in HaCaT cells and also exhibited antiaging effect, as demonstrated by a clinical study. These results suggest that PEP shows potential as a skin antiaging cosmetic product.

Antiaging Potential of Peptides from Underused Marine Bioresources

“…In a scratch-assay wounding model in HaCaT cells, 1 μg/mL of PEP, an antioxidant peptide from fermented Trapa Japonica fruit, significantly promotes wound healing: a 4.4-fold increase in the recovery of the scratched area was observed in PEP-treated cells. These studies also found that marine peptides can boost wound-recovery by increasing migration and proliferation of keratinocytes [ 53 , 54 ].…”

“…Collagen synthesis is considered a key factor in skin aging [ 53 , 70 ]. Sufficient amino acids and oligopeptides binding to the surface of fibroblasts are both important for collagen synthesis [ 71 ].…”

Anti-aging skin and antioxidant assays of protein hydrolysates obtained from salted shrimp fermented with Salinivibrio cibaria BAO-01

“…Several studies have reported that fermentation increases the anti-aging activity of products [34,35]. Jang, Kim et al [34] reported greater values for fermented Trapa japonica fruit than for protein extracted from fresh fruit.…”

“…Several studies have reported that fermentation increases the anti-aging activity of products [34,35]. Jang, Kim et al [34] reported greater values for fermented Trapa japonica fruit than for protein extracted from fresh fruit. The results correspond to earlier findings of Fan, Zhuang et al [36] and Chlapanidas, Faragò et al [37] for protein extracts of marine organisms, both in vitro and in vivo.…”

Topical and nutricosmetic products for healthy hair and dermal antiaging using “dual‐acting” (2 for 1) plant‐based peptides, hormones, and cannabinoids

“…However, the current review is focused on plant‐based ingredients because the cosmetics industry has recognized that consumer preference has shifted toward plant‐based alternatives 30 . Fortunately, in the past few decades research has started to indicate that plant‐derived peptides can also confer positive effects 31 consistent with the endogenous cytokines, such as GHK‐Cu, but with better pharmacokinetics.…”