Antiaging effect of inotodiol on oxidative stress in human dermal fibroblasts

Lee, Jun Young; Won, Gun-Woo; Kim, Mi-Yoon; Oh, Cheong-Hae; Park, Jong-Tae; Park, Jong-Il

doi:10.1016/j.biopha.2022.113311

Cited by 9 publications

(6 citation statements)

References 67 publications

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“…Previously, it has been reported that inotodiol has anti-oxidative and anti-allergic activities in vivo and in vitro [ 32 , 33 , 34 ]. Moreover, recent study shows that inotodiol has anti-aging effects by protecting human dermal fibroblasts by blocking MAPK-NOX5 and NF-κB activation and suppressing aging gene expression [ 16 ]. However, it is still not elucidated if inotodiol affects cytokine expression after stimulation with UV and TNF-α and how inotodiol impacts extracellular matrix production after stress stimulation.…”

Section: Discussionmentioning

confidence: 99%

“…A standard MTT assay was performed to determine cytotoxicity according to the manufacturer’s protocol (M6494, Invitrogen, Camarillo, CA, USA) [ 16 ]. HaCaT cells were seeded at a density of 1 × 10 5 cells/well and incubated for 24 h. Cells were treated with various concentrations (0.6~20 μg/mL) of pure inotodiol and inotodiol concentrate in a 96-well plate.…”

Section: Methodsmentioning

confidence: 99%

“…Inotodiol, a lanostane-type triterpenoid from the Chaga mushroom, has been used as a traditional medicine for diabetes, cardiovascular disorders, and tuberculosis [ 11 , 12 ]. Numerous studies have investigated inotodiol’s biological activities, and it has been proven to have antitumor, anti-inflammatory, antiallergic, antiviral, antiaging, and antioxidant properties [ 13 , 14 , 15 , 16 ]. Recently, there has been a movement to identify novel, safe, and effective molecules that can be used for the treatment of various diseases, including inflammatory diseases.…”

Section: Introductionmentioning

confidence: 99%

“…So far, it has been reported that inotodiol exerts anti-inflammatory activity without showing detrimental effects in in vivo and in vitro studies [ 17 ]. Also, inotodiol shows an anti-aging effect under oxidative stress by inhibiting MAPK-NOX5 and NF-κB signaling pathways [ 16 ]. However, the anti-aging and anti-inflammatory activities of inotodiol under different types of oxidative stress and the impact of inotodiol on collagen and hyaluronan synthesis have not been sufficiently studied.…”

Section: Introductionmentioning

confidence: 99%

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Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses

Park¹,

Nguyen²,

Park³

et al. 2023

IJMS

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Inotodiol, a lanostane-type triterpenoid, and many phytochemicals from Chaga mushrooms have been investigated for various allergic diseases. However, the anti-aging and anti-inflammatory activities of inotodiol under different types of oxidative stress and the impact of inotodiol on collagen and hyaluronan synthesis have not been sufficiently studied. Lanostane triterpenoids-rich concentrate, which contained 10% inotodiol as major (inotodiol concentrate), was prepared from Chaga and compared with pure inotodiol in terms of anti-inflammatory activities on a human keratinocyte cell line, HaCaT cells, under various stimulations such as stimulation with ultraviolet (UV) B or tumor necrosis factor (TNF)-α. In stimulation with TNF-α, interleukin (IL)-1β, IL-6, and IL-8 genes were significantly repressed by 0.44~4.0 μg/mL of pure inotodiol. UVB irradiation induced the overexpression of pro-inflammatory cytokines, but those genes were significantly suppressed by pure inotodiol or inotodiol concentrate. Moreover, pure inotodiol/inotodiol concentrate could also modulate the synthesis of collagen and hyaluronic acid by controlling COL1A2 and HAS2/3 expression, which implies a crucial role for pure inotodiol/inotodiol concentrate in the prevention of skin aging. These results illuminate the anti-inflammatory and anti-aging effects of pure inotodiol/inotodiol concentrate, and it is highly conceivable that pure inotodiol and inotodiol concentrate could be promising natural bioactive substances to be incorporated in therapeutic and beautifying applications.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses

Park¹,

Nguyen²,

Park³

et al. 2023

IJMS

Self Cite

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show abstract

“…Inotodiol, a lanostanetype triterpenoid from the Chaga mushroom, has been used as a traditional medicine for diabetes, cardiovascular disorders, and tuberculosis [11,12]. Numerous studies have investigated inotodiol's biological activities, and it has been proven to have antitumor, anti-inflammatory, antiallergic, antiviral, antiaging, and antioxidant properties [13][14][15][16]. Recently, there has been a movement to identify novel, safe, and effective molecules that can be used for the treatment of various diseases, including inflammatory diseases.…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes HaCaT Cell against Inflammatory and Oxidative Stresses

Park¹,

Nguyen²,

Park³

et al. 2023

Preprint

View full text Add to dashboard Cite

Inotodiol, a lanostane-type triterpenoid, and many phytochemicals from Chaga mushroom have been investigated for various allergic diseases. However, antiaging and anti-inflammatory activities of inotodiol under different types of oxidative stress and impact of inotodiol on collagen and hyaluronan synthesis are not sufficiently studied. Lanostane triterpenoids-rich concentrate, which contained 10% inotodiol as major (the inotodiol concentrate), was prepared from Chaga and compared with pure inotodiol in terms of anti-inflammatory activities on a human keratinocyte cell line, HaCaT cells, under various stimulation such as stimulation with ultraviolet (UV) B or tumor necrosis factor (TNF)-α. In stimulation with TNF-α, interleukin (IL)-1β, IL-6, and IL-8 genes were significantly repressed by 0.44~4.0 μg/mL of pure inotodiol. UVB irradiation induced overexpressions of pro-inflammatory cytokines, but those genes were significantly suppressed by pure inotodiol or inotodiol concentrate. Moreover, pure inotodiol/inotodiol concentrate could also modulate synthesis of collagen and hyaluronic acid by controlling COL1A2 and HAS2/3 expression, which implies a crucial role of pure inotodiol/inotodiol concentrate in prevention of skin aging. These results enlighten anti-inflammatory and anti-aging effect of pure inotodiol/inotodiol concentrate, and it is highly conceivable that pure inotodiol and inotodiol concentrate could be promising natural bioactive substances to be incorporated in therapeutic and beautifying applications.

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Rubia sylvatica anthocyanins protect retinal pigment epithelial cells from H2O2-induced oxidative damage

Chen,

Li,

Yang

et al. 2023

Food Bioscience

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Antiaging effect of inotodiol on oxidative stress in human dermal fibroblasts

Cited by 9 publications

References 67 publications

Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses

Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses

Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes HaCaT Cell against Inflammatory and Oxidative Stresses

Rubia sylvatica anthocyanins protect retinal pigment epithelial cells from H2O2-induced oxidative damage

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