Antiallergic Properties of Biflavonoids Isolated from the Flowers of Mesua ferrea Linn.

Manse, Yoshiaki; Sakamoto, Yusuke; Miyachi, Taiki; Nire, Mitsuyo; Hashimoto, Yoshinori; Chaipech, Saowanee; Pongpiriyadacha, Yutana; Morikawa, Toshio

doi:10.3390/separations9050127

Cited by 5 publications

(5 citation statements)

References 63 publications

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“…Several phytochemical studies have shown that the chemical components of M. ferrea L. flower are phenolic acid [13], flavonoid [13], coumarin [14] and xanthone [13,15]. A previous study reported the anti-AD action of phenolic acids which prevented the development of AD pathology by inhibiting the Aβ aggregation pathway [26].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the crude extract of M. ferrea L. flower exhibits activities related to the pathogenesis of AD, which includes antioxidant [12] and antiinflammation [11]. Several phytochemical studies have shown that the major chemical components of M. ferrea L. are phenolic compounds [13], flavonoids [13], coumarins [14], and xanthones [15]. From the literature review, these components are naturally occurring phytochemicals with heterocyclic structures that display a wide range of biological activities related to AD including anti-acetylcholinesterase, anti-Aβ aggregation, antioxidant, and anti-inflammation.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Ethanol Extract from Mesua ferrea Linn Flower on Alzheimer’s Disease and Its Underlying Mechanism

Plekratoke

Boonyarat

Monthakantirat

et al. 2023

CIMB

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The effects of Mesua ferrea Linn flower (MFE) extract on the pathogenic cascade of Alzheimer’s disease (AD) were determined by an in vitro and cell culture model in the search for a potential candidate for the treatment of AD. The 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay exhibited that the MFE extract had antioxidant activities. According to the Ellman and the thioflavin T method’s result, the extracts could inhibit acetylcholinesterase and β-amyloid (Aβ) aggregation. Studies on neuroprotection in cell culture found that the MFE extract could reduce the death of human neuroblastoma cells (SH-SY5Y) caused by H2O2 and Aβ. Western blot analysis exhibited that the MFE extract alleviated H2O2-induced neuronal cell damage by downregulating the pro-apoptotic proteins, including cleaved caspase-3, Bax, and by enhancing the expression of anti-apoptotic markers including MCl1, BClxl, and survivin. Moreover, MFE extract inhibited the expression of APP, presenilin 1, and BACE, and increased the expression of neprilysin. In addition, the MFE extract could enhance scopolamine-induced memory deficit in mice. Overall, results showed that the MFE extract had several modes of action related to the AD pathogenesis cascade, including antioxidants, anti-acetylcholinesterase, anti-Aβ aggregation, and neuroprotection against oxidative stress and Aβ. Therefore, the M. ferrea L. flower might be a possibility for further development as a medication for AD.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effect of Ethanol Extract from Mesua ferrea Linn Flower on Alzheimer’s Disease and Its Underlying Mechanism

Plekratoke

Boonyarat

Monthakantirat

et al. 2023

CIMB

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“…Flavonoid is a secondary metabolite of plant polyphenol, most of which exist in the form of glycosides in plants, and a few of which exist in the form of free aglycones (oxyglycosides and carbon glycosides). Flavonoids are natural products with signifcant biological activities, including antiviral [14], antiallergic [15], lipid-lowering [16], antibacterial [17], and anti-infammatory efects [18] as well as prevention of cardiovascular and cerebrovascular diseases [19]. At present, there is no systematic study on the biological activity of favonoids from S. vaninii fruit bodies, so this study evaluates the biological activity of favonoids from S. vaninii fruit bodies in vitro.…”

Section: Introductionmentioning

confidence: 99%

Antiradical Activity Studies of Ultrasound-Assisted Extraction of Total Flavonoids from Sanghuangporus vaninii Fruit Bodies

Lin

Dou

Gao

et al. 2023

Journal of Food Biochemistry

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Flavonoids are a class of secondary metabolites present in many plant species. Flavonoids are important active components in Sanghuangporus vaninii. It has attracted widely attention because of its unique antitumor, antioxidation, immunomodulatory, bacteriostatic, and other biological activities. In this study, the Sanghuangporus vaninii fruit bodies are used as raw materials to study the optimal extraction technology of the Sanghuangporus flavonoids. In addition, the antioxidant and bacteriostatic abilities of Sanghuangporus vaninii are analyzed through antioxidant and bacteriostatic experiments. Ultrasound-assisted ethanol extraction is used to extract Sanghuangporus flavonoids. The optimum extraction process was determined by single factor test and response surface analysis, namely, ratio of material to liquid 1 : 60, ethanol concentration 80%, ultrasonic temperature 70°C, and ultrasonic time 40 min. Under these conditions, the flavonoids content is 90.24 ± 0.89 mg/g (on dry weight basis). Using VC as the reference substance, the total flavonoid concentration was tested for its ability to scavenge free radicals such as DPPH, ABTS+, OH, and O2-. The reducing power of total crude flavonoids, the scavenging ability of NO2- , and the antibacterial and anti-inflammatory abilities are also used as evaluation indicators for this experiment. The results showed that the best scavenging rates of Sanghuangporus vaninii flavonoids for DPPH, ABTS+,· OH, and O2-.were, respectively, 92.17 ± 1.33%, 92.49 ± 1.55%, 94.85 ± 1.39%, and 86.01 ± 1.21%. In terms of reducibility, the absorbance of Sanghuangporus vaninii flavonoids at 0.008 mg/ml was 2.65 ± 0.04, which was higher than that of positive control VC at 0.90 ± 0.05. Sanghuangporus vaninii flavonoids at 200 μg/mL could effectively inhibit the NO2- content in vitro to 9.38 ± 0.23 pg/ml. The inhibitory rates of Sanghuangporus vaninii flavonoids on Escherichia coli, Staphylococcus aureus, and Bacillus subtilis were 79.48 ± 1.57%, 85.64 ± 1.22%, and 80.29 ± 0.04%. When the concentration of Sanghuangporus vaninii flavonoids was 200 μg/mL, the concentration of NO was 9.38 ± 0.23 pg/ml.

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“…Several studies revealed that the extract of M. ferrea L. flower showed biological activities related to AD such as anti-inflammation (14), antioxidant (15), anti-Aβ aggregation, and AChE inhibitory action (16). A review of the literature found that kaempferol-3-O-rhamnoside, and quercetin-3-O-rhamnoside are the major compounds isolated from the flower of M. ferrea L. (17). Both kaempferol-3-O-rhamnoside, and quercetin-3-O-rhamnoside have various biological activities, e.g., anti-cancer (18), anti-diabetic (19), and antiviral (20).…”

Section: Introductionmentioning

confidence: 99%

Multi-Target Actions of Flavonoid Derivatives from Mesua ferrea Linn Flower against Alzheimer’s disease Pathogenesis

Plekratoke,

Waiwut,

Yenjai

et al. 2023

BSCM

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OBJECTIVE Kaempferol-3-O-rhamnoside (compound 1) and quercetin- 3-O-rhamnoside (compound 2), two flavonoids isolated from Mesua ferrea L. flowers, were examined for their activities related Alzheimer’s disease (AD) pathogenesis including antioxidant, acetylcholinesterase (AChE) inhibition, anti-beta amyloid (Ab) aggregation and neuroprotection. METHODS The two flavonoids were isolated from M. ferrea L. flowers using the column chromatography technique. Both compounds were evaluated for their effects on AD pathogenesis, including antioxidant action by ABTS assay, AChE inhibition by Ellman’s method, and anti-Ab aggregation by thioflavin T (ThT) assay and neuroprotection by cell base assay. To explain the mechanism of AChE inhibition and anti-Ab aggregation, binding interactions between the test compounds and AChE and Ab were studied in-silico. RESULTS Compounds 1 and 2 showed an ability to scavenge ABTS radicals, with IC50 values of 424.57±2.97 and 308.67±9.90 µM, respectively, and to inhibit AChE function with IC50 values of 769.23±6.23 and 520.64±5.94, respectively. ThT assay indicated that both compounds inhibited Ab aggregation with IC50 values of 406.43±9.95 and 300.69 ±1.18 µM, respectively. The neuroprotection study revealed that the two flavonoids could reduce human neuroblastoma (SH-SY5Y) cell death induced by H2O2. The in-silico study showed that both compounds bound AChE at catalytic anionic and peripheral anionic sites. In addition, the test compounds prevented Ab aggregation by interacting at the central hydrophobic core, the C-terminal hydrophobic region, and the important residues of Ile41. CONCLUSIONS Together, the results showed that kaempferol-3-O- rhamnoside and quercetin-3-O- rhamnoside exhibit multiple mechanisms of action that are involved in the pathogenesis of AD including antioxidant, AChE inhibition, anti-Ab aggregation, and neuroprotection. KEYWORDS flavonoid rhamnosides, Alzheimer’s disease, oxidation, beta amyloid, acetylcholinesterase, molecular docking

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Antiallergic Properties of Biflavonoids Isolated from the Flowers of Mesua ferrea Linn.

Cited by 5 publications

References 63 publications

The Effect of Ethanol Extract from Mesua ferrea Linn Flower on Alzheimer’s Disease and Its Underlying Mechanism

The Effect of Ethanol Extract from Mesua ferrea Linn Flower on Alzheimer’s Disease and Its Underlying Mechanism

Antiradical Activity Studies of Ultrasound-Assisted Extraction of Total Flavonoids from Sanghuangporus vaninii Fruit Bodies

Multi-Target Actions of Flavonoid Derivatives from Mesua ferrea Linn Flower against Alzheimer’s disease Pathogenesis

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